PMID- 34763077
OWN - NLM
STAT- MEDLINE
DCOM- 20220117
LR  - 20220531
IS  - 1879-1107 (Electronic)
IS  - 1096-4959 (Linking)
VI  - 258
DP  - 2022 Feb-Mar
TI  - MicroRNA ssa-mir-196a-4 deceases lgr8 expression in testis development of Chinese 
      tongue sole (Cynoglossus semilaevis).
PG  - 110695
LID - S1096-4959(21)00134-2 [pii]
LID - 10.1016/j.cbpb.2021.110695 [doi]
AB  - MicroRNAs (miRNAs) contribute to gonadal development in animals. However, there 
      is little information about miRNA regulation function involved in gonadal 
      development in fish. Our group previously identified sex-related miRNAs of 
      Chinese tongue sole (Cynoglossus semilaevis) during sex determination and 
      differentiation by small RNA sequencing. In the present study, we characterized 
      ssa-mir-196a-4 and its expression in testis and verified its interaction with 
      lgr8. miRNA ssa-mir-196a-4 precursor was predicted to have a typical hairpin 
      structure and highly conserved among various fish species. Fluorescence in situ 
      hybridization (FISH) of ssa-mir-196a-4 in the testis of Chinese tongue sole 
      showed that it is mainly expressed in the cytoplasm of Sertoli cells. We 
      determined that ssa-mir-196a-4 interacted with lgr8 by bioinformatics analysis 
      using miRanda software. According to the dual-luciferase gene reporter assay, 
      lgr8 is a direct target of ssa-mir-196a-4. Overexpression of ssa-mir-196a-4 in 
      the cells of the testis cell line of Chinese tongue sole decreased the expression 
      levels of lgr8 messenger RNA (mRNA) and protein by targeting its coding sequence 
      (CDS) region. These results suggest that ssa-mir-196a-4 acts as a 
      post-transcriptional regulator of lgr8 and plays an important role in developing 
      testes of Chinese tongue sole.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Tang, Lili
AU  - Tang L
AD  - Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, 
      Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food 
      Production Processes, Pilot National Laboratory for Marine Science and Technology 
      (Qingdao), Qingdao 266237, China.
FAU - You, Wuxin
AU  - You W
AD  - Single-Cell Center CAS Key Laboratory of Biofuels and Shandong Key Laboratory of 
      Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, 
      Chinese Academy of Sciences, Qingdao, Shandong, China.
FAU - Wang, Qian
AU  - Wang Q
AD  - Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, 
      Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food 
      Production Processes, Pilot National Laboratory for Marine Science and Technology 
      (Qingdao), Qingdao 266237, China.
FAU - Huang, Fei
AU  - Huang F
AD  - Genosys, Inc., Shenzhen, China.
FAU - Shao, Changwei
AU  - Shao C
AD  - Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, 
      Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food 
      Production Processes, Pilot National Laboratory for Marine Science and Technology 
      (Qingdao), Qingdao 266237, China. Electronic address: shaocw@ysfri.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20211109
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Fish Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
MH  - Animals
MH  - China
MH  - Fish Proteins/*genetics
MH  - Fishes/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *MicroRNAs/genetics
MH  - Receptors, G-Protein-Coupled/*genetics
MH  - *Testis/growth & development
OTO - NOTNLM
OT  - Chinese tongue sole
OT  - MicroRNA ssa-mir-196a-4
OT  - Testis development
OT  - lgr8
EDAT- 2021/11/12 06:00
MHDA- 2022/01/18 06:00
CRDT- 2021/11/11 20:15
PHST- 2021/04/15 00:00 [received]
PHST- 2021/10/06 00:00 [revised]
PHST- 2021/11/03 00:00 [accepted]
PHST- 2021/11/12 06:00 [pubmed]
PHST- 2022/01/18 06:00 [medline]
PHST- 2021/11/11 20:15 [entrez]
AID - S1096-4959(21)00134-2 [pii]
AID - 10.1016/j.cbpb.2021.110695 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2022 Feb-Mar;258:110695. doi: 
      10.1016/j.cbpb.2021.110695. Epub 2021 Nov 9.