PMID- 34763485
OWN - NLM
STAT- MEDLINE
DCOM- 20211115
LR  - 20220531
IS  - 2224-5839 (Electronic)
IS  - 2224-5820 (Linking)
VI  - 10
IP  - 10
DP  - 2021 Oct
TI  - Mechanism research on the interaction regulation of Escherichia and IFN-gamma for the 
      occurrence of T2DM.
PG  - 10391-10400
LID - 10.21037/apm-21-2318 [doi]
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is a major social and public health 
      problem which may be induced by intestinal flora imbalance through inflammatory 
      response, and the specific mechanism remains unclear. In this study, we aim to 
      explore the interaction network of intestinal flora and cell inflammation in 
      T2DM. METHODS: This a case-control study. Patients with T2DM was the case group 
      and healthy people as control. The differences of cytokine expression levels 
      between patients with T2DM and healthy controls were assessed by using flow 
      cytometry. The diversity and abundance of intestinal flora were evaluated by 
      using 16S rRNA three-generation full-length sequencing technology. RESULTS: A 
      total of 29 patients with T2DM and 28 healthy controls were included for 
      analysis. Compared with the healthy control group, the expression levels of 
      plasma cytokine interleukin-2 (IL-2) (P=0.0000006), IL-6 (P=0.000193), tumor 
      necrosis factor alpha (TNF-alpha) (P=0.016), interferon-gamma (IFN-gamma) (P=0.000036) and 
      interleukin-17 (IL-17) (P=0.004) were significantly up-regulated in T2DM 
      patients, and the abundance of Megamonas_funiformis (P=0.0016) and Escherichia 
      (P=0.049) in the intestine were significantly increased. In contrast, the 
      abundance of Bacteroides_stercoris (P=0.0068), Bacteroides_uniformis (P=0.033), 
      and Phascolarctobacterium_faecium (P=0.033) were decreased in T2DM patients. 
      Further, differentially expressed Escherichia had a positive correlation with 
      IFN-gamma (r=0.73) by Pearson correlation analysis. CONCLUSIONS: The interaction 
      network between the intestinal bacteria Escherichia and the cytokine IFN-gamma may 
      drive inflammation in visceral adipose tissue (VAT), indicating insulin signal 
      transduction can be inhibited in adipocytes to induce insulin resistance.
FAU - Lv, Meijun
AU  - Lv M
AD  - Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of 
      Traditional Chinese Medicine, Chengdu, China; School of Medical Technology, 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Li, Lisha
AU  - Li L
AD  - Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of 
      Traditional Chinese Medicine, Chengdu, China.
FAU - Li, Weidong
AU  - Li W
AD  - Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of 
      Traditional Chinese Medicine, Chengdu, China; School of Medical Technology, 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Yang, Fujie
AU  - Yang F
AD  - Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of 
      Traditional Chinese Medicine, Chengdu, China; School of Medical Technology, 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Hu, Qiongying
AU  - Hu Q
AD  - Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of 
      Traditional Chinese Medicine, Chengdu, China; School of Medical Technology, 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Xiong, Daqian
AU  - Xiong D
AD  - Department of Laboratory Medicine, Affiliated Hospital of Chengdu University of 
      Traditional Chinese Medicine, Chengdu, China; School of Medical Technology, 
      Chengdu University of Traditional Chinese Medicine, Chengdu, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Palliat Med
JT  - Annals of palliative medicine
JID - 101585484
RN  - 0 (IFNG protein, human)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Case-Control Studies
MH  - *Diabetes Mellitus, Type 2/metabolism/microbiology
MH  - *Escherichia
MH  - Humans
MH  - Interferon-gamma/genetics/*metabolism
MH  - Intestines/*microbiology
MH  - RNA, Ribosomal, 16S
OTO - NOTNLM
OT  - Type 2 diabetes mellitus (T2DM)
OT  - cytokines
OT  - insulin resistancet
OT  - intestinal flora
EDAT- 2021/11/13 06:00
MHDA- 2021/11/16 06:00
CRDT- 2021/11/12 05:27
PHST- 2021/06/28 00:00 [received]
PHST- 2021/09/28 00:00 [accepted]
PHST- 2021/11/12 05:27 [entrez]
PHST- 2021/11/13 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
AID - 10.21037/apm-21-2318 [doi]
PST - ppublish
SO  - Ann Palliat Med. 2021 Oct;10(10):10391-10400. doi: 10.21037/apm-21-2318.