PMID- 34764737
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211113
IS  - 1319-562X (Print)
IS  - 2213-7106 (Electronic)
IS  - 2213-7106 (Linking)
VI  - 28
IP  - 11
DP  - 2021 Nov
TI  - Overexpression of CXCL8 gene in Saudi colon cancer patients.
PG  - 6045-6049
LID - 10.1016/j.sjbs.2021.09.031 [doi]
AB  - Colorectal cancer (CRC) is one of the leading causes of death in Saudi Arabia. 
      CRC mostly affects older age groups, but now a days it also appears frequently at 
      a young age. However, the complete genetic etiology of CRC remains unknown. To 
      identify the genetic factors responsible for this cancer type and to search for 
      biomarkers for early diagnosis and prevention, we collected sixteen CRC tumor 
      tissue samples and six normal colon tissues and extracted mRNA and synthesized 
      cDNA. We then performed microarray transcriptomic profiling of Saudi patients 
      with colon cancer. Gene expression was analyzed using Partek Genomics Suite, and 
      principal component analysis (PCA) was performed to separate the different 
      clusters of colon cancer and healthy tissues. Distinct differences in gene 
      expression profiles were observed between colon cancer and normal tissue samples. 
      Subsequently, we validated gene expression using real-time PCR. We found that the 
      C-X-C motif chemokine ligand 8 (CXCL8) gene was expressed most in CRC samples. 
      CXCL8 expressed 25.6 folds more in CRC tissues than in healthy tissues. In 
      conclusion, we found that CXCL8 is the chief biomarker gene that is expressed 
      most in CRC and plays an important role in tumor progression and metastasis.
CI  - (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University.
FAU - Rasool, Mahmood
AU  - Rasool M
AD  - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical 
      Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
AD  - Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah, Saudi Arabia.
FAU - Natesan Pushparaj, Peter
AU  - Natesan Pushparaj P
AD  - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical 
      Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
AD  - Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah, Saudi Arabia.
FAU - Karim, Sajjad
AU  - Karim S
AD  - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical 
      Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
AD  - Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20210917
PL  - Saudi Arabia
TA  - Saudi J Biol Sci
JT  - Saudi journal of biological sciences
JID - 101543796
PMC - PMC8568844
OTO - NOTNLM
OT  - Biomarker
OT  - CXCL8
OT  - CXCR1
OT  - Colorectal Cancer
OT  - Microarray
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2021/11/13 06:00
MHDA- 2021/11/13 06:01
PMCR- 2021/09/17
CRDT- 2021/11/12 06:59
PHST- 2021/08/10 00:00 [received]
PHST- 2021/09/08 00:00 [revised]
PHST- 2021/09/12 00:00 [accepted]
PHST- 2021/11/12 06:59 [entrez]
PHST- 2021/11/13 06:00 [pubmed]
PHST- 2021/11/13 06:01 [medline]
PHST- 2021/09/17 00:00 [pmc-release]
AID - S1319-562X(21)00832-9 [pii]
AID - 10.1016/j.sjbs.2021.09.031 [doi]
PST - ppublish
SO  - Saudi J Biol Sci. 2021 Nov;28(11):6045-6049. doi: 10.1016/j.sjbs.2021.09.031. 
      Epub 2021 Sep 17.