PMID- 34768855
OWN - NLM
STAT- MEDLINE
DCOM- 20211229
LR  - 20211229
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 21
DP  - 2021 Oct 22
TI  - Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled 
      Haploid.
LID - 10.3390/ijms222111424 [doi]
LID - 11424
AB  - The molecular karyotype could represent the basic genetic make-up in a cell 
      nucleus of an organism or species. A doubled haploid (DH) is a genotype formed 
      from the chromosome doubling of haploid cells. In the present study, molecular 
      karyotype analysis of the poplar hybrid Populus simonii x P. nigra (P. xiaohei) 
      and the derived doubled haploids was carried out with labeled telomeres, rDNA, 
      and two newly repetitive sequences as probes by fluorescence in situ 
      hybridization (FISH). The tandem repeats, pPC349_XHY and pPD284_XHY, with 
      high-sequence homology were used, and the results showed that they presented the 
      colocalized distribution signal in chromosomes. For P. xiaohei, pPD284_XHY 
      produced hybridizations in chromosomes 1, 5, 8, and 9 in the hybrid. The 
      combination of pPD284_XHY, 45S rDNA, and 5S rDNA distinctly distinguished six 
      pairs of chromosomes, and the three pairs of chromosomes showed a significant 
      difference in the hybridization between homologous chromosomes. The repeat probes 
      used produced similar FISH hybridizations in the DH; nevertheless, pPD284_XHY 
      generated an additional hybridization site in the telomere region of chromosome 
      14. Moreover, two pairs of chromosomes showed differential hybridization 
      distributions between homologous chromosomes. Comparisons of the distinguished 
      chromosomes between hybrid and DH poplar showed that three pairs of chromosomes 
      in the DH presented hybridization patterns that varied from those of the hybrid. 
      The No. 8 chromosome in DH and one of the homologous chromosomes in P. xiaohei 
      shared highly similar FISH patterns, which suggested the possibility of intact or 
      mostly partial transfer of the chromosome between the hybrid and DH. Our study 
      will contribute to understanding the genetic mechanism of chromosomal variation 
      in P. xiaohei and derived DH plants.
FAU - Liu, Bo
AU  - Liu B
AUID- ORCID: 0000-0002-9414-3461
AD  - Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute 
      of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.
FAU - Wang, Sui
AU  - Wang S
AD  - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry 
      University, Harbin 150040, China.
AD  - Key Laboratory of Soybean Biology of Ministry of Education China, Northeast 
      Agricultural University, Harbin 150030, China.
FAU - Tao, Xiaoyan
AU  - Tao X
AD  - Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute 
      of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.
AD  - College of Life Sciences, University of Chinese Academy of Sciences, Beijing 
      100049, China.
FAU - Liu, Caixia
AU  - Liu C
AD  - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry 
      University, Harbin 150040, China.
FAU - Qu, Guanzheng
AU  - Qu G
AD  - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry 
      University, Harbin 150040, China.
FAU - Dou, Quanwen
AU  - Dou Q
AD  - Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute 
      of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.
AD  - Key Laboratory of Crop Molecular Breeding Qinghai Province, Northwest Institute 
      of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.
LA  - eng
GR  - K2019102/open-end funds of State Key Laboratory of Tree Genetics and Breeding, 
      Northeast Forestry University/
GR  - 2572020DY24/Fundamental Research Funds for the Central Universities/
PT  - Journal Article
DEP - 20211022
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Chimera/*genetics
MH  - Chromosomes, Plant/genetics
MH  - Genome, Plant/*genetics
MH  - Genotype
MH  - Karyotype
MH  - Karyotyping
MH  - Populus/classification/*genetics
MH  - Tandem Repeat Sequences/*genetics
PMC - PMC8584087
OTO - NOTNLM
OT  - FISH
OT  - Populus
OT  - Populus simonii x P. nigra
OT  - doubled haploid
OT  - karyotype
OT  - repetitive sequence
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/14 06:00
MHDA- 2021/12/30 06:00
PMCR- 2021/10/22
CRDT- 2021/11/13 01:03
PHST- 2021/09/03 00:00 [received]
PHST- 2021/10/19 00:00 [revised]
PHST- 2021/10/21 00:00 [accepted]
PHST- 2021/11/13 01:03 [entrez]
PHST- 2021/11/14 06:00 [pubmed]
PHST- 2021/12/30 06:00 [medline]
PHST- 2021/10/22 00:00 [pmc-release]
AID - ijms222111424 [pii]
AID - ijms-22-11424 [pii]
AID - 10.3390/ijms222111424 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Oct 22;22(21):11424. doi: 10.3390/ijms222111424.