PMID- 34771134
OWN - NLM
STAT- MEDLINE
DCOM- 20211203
LR  - 20211214
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 21
DP  - 2021 Nov 6
TI  - Subcellular Localization of Connexin 26 in Cardiomyocytes and in 
      Cardiomyocyte-Derived Extracellular Vesicles.
LID - 10.3390/molecules26216726 [doi]
LID - 6726
AB  - Connexins (Cxs) are a family of membrane-spanning proteins, expressed in 
      vertebrates and named according to their molecular weight. They are involved in 
      tissue homeostasis, and they function by acting at several communication levels. 
      Cardiac Cxs are responsible for regular heart function and, among them, Cx26 and 
      Cx43 are widely expressed throughout the heart. Cx26 is present in vessels, as 
      well as in cardiomyocytes, and its localization is scattered all over the cell 
      aside from at the intercalated discs as is the case for the other cardiac Cxs. 
      However, having been found in cardiomyocytes only recently, both its subcellular 
      localization and its functional characterization in cardiomyocytes remain poorly 
      understood. Therefore, in this study we aimed to obtain further data on the 
      localization of Cx26 at the subcellular level. Our TEM immunogold analyses were 
      performed on rat heart ventricles and differentiated H9c2 cardiac cell sections 
      as well as on differentiated H9c2 derived extracellular vesicles. The results 
      confirmed the absence of Cx26 at intercalated discs and showed the presence of 
      Cx26 at the level of different subcellular compartments. The peculiar 
      localization at the level of extracellular vesicles suggested a specific role for 
      cardiac Cx26 in inter-cellular communication in an independent gap junction 
      manner.
FAU - Falleni, Alessandra
AU  - Falleni A
AUID- ORCID: 0000-0001-6492-6038
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
FAU - Moscato, Stefania
AU  - Moscato S
AUID- ORCID: 0000-0001-9283-6902
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
FAU - Sabbatini, Antonietta R M
AU  - Sabbatini ARM
AUID- ORCID: 0000-0002-6375-7778
AD  - Department of Surgical, Medical, Molecular Pathology and of Emergency Medicine, 
      University of Pisa, 56126 Pisa, Italy.
FAU - Bernardeschi, Margherita
AU  - Bernardeschi M
AUID- ORCID: 0000-0003-2650-1834
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
FAU - Bianchi, Francesco
AU  - Bianchi F
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
FAU - Cecchettini, Antonella
AU  - Cecchettini A
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
AD  - Institute of Clinical Physiology (IFC), National Research Council (CNR), 56126 
      Pisa, Italy.
FAU - Mattii, Letizia
AU  - Mattii L
AUID- ORCID: 0000-0001-6835-7987
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
LA  - eng
PT  - Journal Article
DEP - 20211106
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 127120-53-0 (Connexin 26)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Connexin 26/*analysis/metabolism
MH  - Extracellular Vesicles/*chemistry/metabolism
MH  - Gap Junctions/chemistry/metabolism
MH  - Myocytes, Cardiac/*chemistry/metabolism
MH  - Rats
PMC - PMC8587102
OTO - NOTNLM
OT  - H9c2 cells
OT  - cardiomyocyte
OT  - connexin26
OT  - extracellular vesicles
OT  - immunoelectron microscopy
OT  - rat heart
OT  - ultrastructure
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/14 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/11/06
CRDT- 2021/11/13 01:13
PHST- 2021/09/14 00:00 [received]
PHST- 2021/10/26 00:00 [revised]
PHST- 2021/11/03 00:00 [accepted]
PHST- 2021/11/13 01:13 [entrez]
PHST- 2021/11/14 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/11/06 00:00 [pmc-release]
AID - molecules26216726 [pii]
AID - molecules-26-06726 [pii]
AID - 10.3390/molecules26216726 [doi]
PST - epublish
SO  - Molecules. 2021 Nov 6;26(21):6726. doi: 10.3390/molecules26216726.