PMID- 34771149
OWN - NLM
STAT- MEDLINE
DCOM- 20211203
LR  - 20230518
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 21
DP  - 2021 Nov 8
TI  - Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular 
      Matrices.
LID - 10.3390/molecules26216740 [doi]
LID - 6740
AB  - Tissue microenvironments are rich in signaling molecules. However, factors in the 
      tissue matrix that can serve as tissue-specific cues for engineering pancreatic 
      tissues have not been thoroughly identified. In this study, we performed a 
      comprehensive proteomic analysis of porcine decellularized pancreatic 
      extracellular matrix (dpECM). By profiling dpECM collected from subjects of 
      different ages and genders, we showed that the detergent-free decellularization 
      method developed in this study permits the preservation of approximately 62.4% 
      more proteins than a detergent-based method. In addition, we demonstrated that 
      dpECM prepared from young pigs contained approximately 68.5% more extracellular 
      matrix proteins than those prepared from adult pigs. Furthermore, we categorized 
      dpECM proteins by biological process, molecular function, and cellular component 
      through gene ontology analysis. Our study results also suggested that the protein 
      composition of dpECM is significantly different between male and female animals 
      while a KEGG enrichment pathway analysis revealed that dpECM protein profiling 
      varies significantly depending on age. This study provides the proteome of 
      pancreatic decellularized ECM in different animal ages and genders, which will 
      help identify the bioactive molecules that are pivotal in creating 
      tissue-specific cues for engineering tissues in vitro.
FAU - Hu, Ming
AU  - Hu M
AD  - Department of Biomedical Engineering, Thomas J. Watson College of Engineering and 
      Applied Sciences, Binghamton University, Binghamton, NY 13902, USA.
FAU - Bi, Huanjing
AU  - Bi H
AD  - Department of Biomedical Engineering, Thomas J. Watson College of Engineering and 
      Applied Sciences, Binghamton University, Binghamton, NY 13902, USA.
FAU - Moffat, Deana
AU  - Moffat D
AD  - Department of Biomedical Engineering, Thomas J. Watson College of Engineering and 
      Applied Sciences, Binghamton University, Binghamton, NY 13902, USA.
FAU - Blystone, Margaret
AU  - Blystone M
AD  - Department of Biomedical Engineering, Thomas J. Watson College of Engineering and 
      Applied Sciences, Binghamton University, Binghamton, NY 13902, USA.
FAU - DeCostanza, Lillian
AU  - DeCostanza L
AD  - Department of Biomedical Engineering, Thomas J. Watson College of Engineering and 
      Applied Sciences, Binghamton University, Binghamton, NY 13902, USA.
FAU - Alayi, Tchilabalo
AU  - Alayi T
AD  - Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical 
      Sciences, Binghamton University, Binghamton, NY 13902, USA.
FAU - Ye, Kaiming
AU  - Ye K
AUID- ORCID: 0000-0001-7736-9875
AD  - Department of Biomedical Engineering, Thomas J. Watson College of Engineering and 
      Applied Sciences, Binghamton University, Binghamton, NY 13902, USA.
AD  - Center of Biomanufacturing for Regenerative Medicine, Binghamton University, 
      Binghamton, NY 13902, USA.
FAU - Hathout, Yetrib
AU  - Hathout Y
AD  - Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical 
      Sciences, Binghamton University, Binghamton, NY 13902, USA.
FAU - Jin, Sha
AU  - Jin S
AUID- ORCID: 0000-0002-8033-8110
AD  - Department of Biomedical Engineering, Thomas J. Watson College of Engineering and 
      Applied Sciences, Binghamton University, Binghamton, NY 13902, USA.
AD  - Center of Biomanufacturing for Regenerative Medicine, Binghamton University, 
      Binghamton, NY 13902, USA.
LA  - eng
GR  - CBET 1928855/National Science Foundation/
PT  - Journal Article
DEP - 20211108
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - *Computational Biology
MH  - Extracellular Matrix/*chemistry
MH  - Pancreas/*chemistry
MH  - Proteins/*analysis
MH  - *Proteomics
MH  - Swine
MH  - Tissue Engineering
PMC - PMC8588251
OTO - NOTNLM
OT  - KEGG pathway
OT  - age and gender
OT  - bioinformatics
OT  - decellularization
OT  - pancreatic extracellular matrix proteins
OT  - proteomics
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/14 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/11/08
CRDT- 2021/11/13 01:13
PHST- 2021/09/04 00:00 [received]
PHST- 2021/11/04 00:00 [revised]
PHST- 2021/11/06 00:00 [accepted]
PHST- 2021/11/13 01:13 [entrez]
PHST- 2021/11/14 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/11/08 00:00 [pmc-release]
AID - molecules26216740 [pii]
AID - molecules-26-06740 [pii]
AID - 10.3390/molecules26216740 [doi]
PST - epublish
SO  - Molecules. 2021 Nov 8;26(21):6740. doi: 10.3390/molecules26216740.