PMID- 34773653
OWN - NLM
STAT- MEDLINE
DCOM- 20220118
LR  - 20220531
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 57
IP  - 1
DP  - 2022 Jan
TI  - Activation of GATA-binding protein 4 regulates monocyte chemoattractant protein-1 
      and chemotaxis in periodontal ligament cells.
PG  - 195-204
LID - 10.1111/jre.12953 [doi]
AB  - BACKGROUND AND OBJECTIVES: Periodontitis is a chronic inflammatory disease of 
      periodontal supporting tissues. The persistent inflammatory reaction depends on 
      the release of chemokines to continuously recruit inflammation cells. 
      GATA-binding protein 4 (GATA4) exerts effects on senescence and inflammation, 
      while its role in periodontitis is far from clear. The present study aims to 
      address the effect of GATA4 on regulating chemokines and the chemotaxis in 
      periodontitis. MATERIAL AND METHODS: Periodontitis rat models were constructed to 
      detect the expression of GATA4 and the chemokine monocyte chemoattractant 
      protein-1 (MCP-1) by immunohistochemistry. Lipopolysaccharide (LPS)-stimulated 
      human periodontal ligament (PDL) cells and GATA4-knockdown by siRNA transient 
      transfection PDL cells were used to explore the correlation between GATA4 and 
      chemokines. Transwell assay was performed to detect the role of GATA4 for the 
      recruitment effect of chemokines on macrophages. Mitogen-activated protein kinase 
      (MAPK) inhibitors were scheduled to intervene in LPS-stimulated PDL cells to 
      examine the association between MAPK signaling pathways and GATA4. The expression 
      of GATA4, chemokines, or MAPK signaling molecules was determined by quantitative 
      real-time polymerase chain reaction, western blotting, or cell 
      immunofluorescence. RESULTS: The expression of GATA4 and MCP-1 was significantly 
      increased in periodontitis rat models and in LPS-stimulated PDL cells. Knockdown 
      GATA4 inhibited the expression of GATA4 and MCP-1 as well as suppressed the 
      recruitment of macrophage in LPS-stimulated PDL cells. Inhibitors of p38 and 
      ERK1/2 signaling pathways significantly downregulated the increased expression of 
      GATA4 and MCP-1 induced by LPS in PDL cells. CONCLUSIONS: GATA-binding protein 4 
      could act as an upstream regulator of MCP-1 and as a downstream regulator of p38 
      and ERK1/2 signaling pathways to initiate inflammation response and regulate 
      chemotaxis during the progression of periodontitis.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Zhu, Ningjing
AU  - Zhu N
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Zheng, Xueqing
AU  - Zheng X
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Qiao, Weiwei
AU  - Qiao W
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Huang, Wushuang
AU  - Huang W
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Li, Ruiqi
AU  - Li R
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Song, Yaling
AU  - Song Y
AUID- ORCID: 0000-0003-0747-7381
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
LA  - eng
GR  - 81670976/National Natural Science Foundation of China/
GR  - [2014]160/Bureau of Science and Technology of Wuhan/
PT  - Journal Article
DEP - 20211113
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (GATA4 Transcription Factor)
RN  - 0 (Gata4 protein, rat)
RN  - 0 (Lipopolysaccharides)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Chemokine CCL2/genetics/metabolism
MH  - *Chemotaxis
MH  - GATA4 Transcription Factor/*metabolism
MH  - Lipopolysaccharides
MH  - *Periodontal Ligament/cytology
MH  - Rats
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
OTO - NOTNLM
OT  - GATA4
OT  - MAPK signaling pathways
OT  - MCP-1
OT  - chemotaxis
OT  - periodontitis
EDAT- 2021/11/14 06:00
MHDA- 2022/01/19 06:00
CRDT- 2021/11/13 12:12
PHST- 2021/10/14 00:00 [revised]
PHST- 2021/09/15 00:00 [received]
PHST- 2021/10/30 00:00 [accepted]
PHST- 2021/11/14 06:00 [pubmed]
PHST- 2022/01/19 06:00 [medline]
PHST- 2021/11/13 12:12 [entrez]
AID - 10.1111/jre.12953 [doi]
PST - ppublish
SO  - J Periodontal Res. 2022 Jan;57(1):195-204. doi: 10.1111/jre.12953. Epub 2021 Nov 
      13.