PMID- 34775152
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 1873-2550 (Electronic)
IS  - 0304-4017 (Linking)
VI  - 300
DP  - 2021 Dec
TI  - Hematological, immunological, and polyamines alterations in the concomitant 
      occurrence of Fasciola gigantica and hepatic leiomyoma in cattle.
PG  - 109617
LID - S0304-4017(21)00277-6 [pii]
LID - 10.1016/j.vetpar.2021.109617 [doi]
AB  - No previous studies have investigated the polyamines alterations during 
      fascioliasis due to F. gigantica in ruminants. This study was therefore carried 
      out to find out the possible relationship between the extent of liver destruction 
      and leiomyoma and some hematological and immunological parameters and polyamines 
      alterations in F. gigantica infection. Fifty cattle with liver fascioliasis and 
      fifteen healthy cattle were selected for the study. For the histopathological 
      study, liver tissue samples were stained with hematoxylin and eosin (H&E) and 
      Masson's Trichrome methods. The leiomyoma suspected specimens were 
      immunohistochemically stained for smooth muscle actin and desmin. Different 
      hematological parameters were investigated in infected and non-infected animals. 
      Furthermore, levels of putrescine, spermidine, and spermine were measured in 
      homogenized liver samples. Serum IL-4 and TNF-alpha levels were also evaluated. By 
      histological examination, the lesions were noted in all the infected specimens. 
      These lesions were varied from leiomyoma, chronic catarrhal cholangitis, 
      arteriosclerosis, telangiectasia, and fresh migratory tunnels filled with RBC and 
      eosinophils. Comparison of hemogram results between infected and non-infected 
      groups revealed a significant decrease in red blood cell counts (RBC), mean 
      corpuscular hemoglobin concentration (MCHC), and platelet count (PLT) in infected 
      animals. Also, a significant elevation in mean corpuscular volume (MCV) 
      concentration was detected in infected animals. The putrescine and spermine 
      levels of the infected animals were significantly higher than the non-infected 
      animals. Although spermidine was increased in infected livers, its elevation was 
      not significant. Based on the results, the level of IL-4 and TNF-alpha was not 
      significantly changed in infected animals. In conclusion, the concurrent 
      occurrence of leiomyoma and fascioliasis due to F. gigantica and polyamines 
      elevation (putrescine and spermine) is reported for the first time. The role of 
      polyamines in the concurrent occurrence of leiomyoma and fascioliasis is an area 
      for future research.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Shahvazi, Safieh
AU  - Shahvazi S
AD  - Department of Pathology, Faculty of Veterinary Medicine, Shahid Chamran 
      University of Ahvaz, Ahvaz, Iran.
FAU - Esmaeilzadeh, Saleh
AU  - Esmaeilzadeh S
AD  - Department of Pathology, Faculty of Veterinary Medicine, Shahid Chamran 
      University of Ahvaz, Ahvaz, Iran. Electronic address: s_esmaeilzadeh@yahoo.com.
FAU - Bahrami, Somayeh
AU  - Bahrami S
AD  - Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran 
      University of Ahvaz, Ahvaz, Iran.
FAU - Najafzadeh, Hossein
AU  - Najafzadeh H
AD  - Cellular and Molecular Biology Research Center, Health Research Institute, Babol 
      University of Medical Sciences, Babol, Iran.
LA  - eng
PT  - Journal Article
DEP - 20211105
PL  - Netherlands
TA  - Vet Parasitol
JT  - Veterinary parasitology
JID - 7602745
RN  - 0 (Polyamines)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Cattle Diseases
MH  - *Fasciola
MH  - *Fascioliasis/veterinary
MH  - *Leiomyoma/veterinary
MH  - Polyamines
OTO - NOTNLM
OT  - Cattle
OT  - Fasciola gigantica
OT  - Leiomyoma
OT  - Liver flukes
OT  - Polyamines
EDAT- 2021/11/15 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/11/14 20:56
PHST- 2021/04/18 00:00 [received]
PHST- 2021/11/02 00:00 [revised]
PHST- 2021/11/03 00:00 [accepted]
PHST- 2021/11/15 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/11/14 20:56 [entrez]
AID - S0304-4017(21)00277-6 [pii]
AID - 10.1016/j.vetpar.2021.109617 [doi]
PST - ppublish
SO  - Vet Parasitol. 2021 Dec;300:109617. doi: 10.1016/j.vetpar.2021.109617. Epub 2021 
      Nov 5.