PMID- 34778237
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220401
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 9
DP  - 2021
TI  - Bioluminescent Sensors for Ca(++) Flux Imaging and the Introduction of a New 
      Intensity-Based Ca(++) Sensor.
PG  - 773353
LID - 10.3389/fbioe.2021.773353 [doi]
LID - 773353
AB  - Sensitive detection of biological events is a goal for the design and 
      characterization of sensors that can be used in vitro and in vivo. One important 
      second messenger is Ca(++) which has been a focus of using genetically encoded 
      Ca(++) indicators (GECIs) within living cells or intact organisms in vivo. An 
      ideal GECI would exhibit high signal intensity, excellent signal-to-noise ratio 
      (SNR), rapid kinetics, a large dynamic range within relevant physiological 
      conditions, and red-shifted emission. Most available GECIs are based on 
      fluorescence, but bioluminescent GECIs have potential advantages in terms of 
      avoiding tissue autofluorescence, phototoxicity, photobleaching, and spectral 
      overlap, as well as enhancing SNR. Here, we summarize current progress in the 
      development of bioluminescent GECIs and introduce a new and previously 
      unpublished biosensor. Because these biosensors require a substrate, we also 
      describe the pros and cons of various substrates used with these sensors. The 
      novel GECI that is introduced here is called CalBiT, and it is a Ca(++) indicator 
      based on the functional complementation of NanoBiT which shows a high dynamic 
      change in response to Ca(++) fluxes. Here, we use CalBiT for the detection of 
      Ca(++) fluctuations in cultured cells, including its ability for real-time 
      imaging in living cells.
CI  - Copyright (c) 2021 Yang and Johnson.
FAU - Yang, Jie
AU  - Yang J
AD  - Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for 
      Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
FAU - Johnson, Carl Hirschie
AU  - Johnson CH
AD  - Department of Biological Sciences, Vanderbilt University, Nashville, TN, United 
      States.
LA  - eng
GR  - P50 HD103537/HD/NICHD NIH HHS/United States
GR  - R21 MH116150/MH/NIMH NIH HHS/United States
PT  - Journal Article
DEP - 20211027
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC8578923
OTO - NOTNLM
OT  - GECIs
OT  - bioluminescent imaging
OT  - luciferin
OT  - nanoluc
OT  - real-time imaging
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest. The handling editor VRV declared a past co-authorship with 
      one of the authors CHJ.
EDAT- 2021/11/16 06:00
MHDA- 2021/11/16 06:01
PMCR- 2021/01/01
CRDT- 2021/11/15 07:09
PHST- 2021/09/09 00:00 [received]
PHST- 2021/10/13 00:00 [accepted]
PHST- 2021/11/15 07:09 [entrez]
PHST- 2021/11/16 06:00 [pubmed]
PHST- 2021/11/16 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 773353 [pii]
AID - 10.3389/fbioe.2021.773353 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2021 Oct 27;9:773353. doi: 10.3389/fbioe.2021.773353. 
      eCollection 2021.