PMID- 34782938
OWN - NLM
STAT- MEDLINE
DCOM- 20221215
LR  - 20231213
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 84
IP  - 4
DP  - 2022 Nov
TI  - Whole-Genome Resequencing of Spontaneous Oxidative Stress-Resistant Mutants 
      Reveals an Antioxidant System of Bradyrhizobium japonicum Involved in Soybean 
      Colonization.
PG  - 1133-1140
LID - 10.1007/s00248-021-01925-2 [doi]
AB  - Soybean is the most inoculant-consuming crop in the world, carrying strains 
      belonging to the extremely related species Bradyrhizobium japonicum and 
      Bradyrhizobium diazoefficiens. Currently, it is well known that B. japonicum has 
      higher efficiency of soybean colonization than B. diazoefficiens, but the 
      molecular mechanism underlying this differential symbiotic performance remains 
      unclear. In the present study, genome resequencing of four spontaneous oxidative 
      stress-resistant mutants derived from the commercial strain B. japonicum E109 
      combined with molecular and physiological studies allowed identifying an 
      antioxidant cluster (BjAC) containing a transcriptional regulator (glxA) that 
      controls the expression of a catalase (catA) and a phosphohydrolase (yfbR) 
      related to the hydrolysis of hydrogen peroxide and oxidized nucleotides, 
      respectively. Integrated synteny and phylogenetic analyses supported the fact 
      that BjAC emergence in the B. japonicum lineage occurred after its divergence 
      from the B. diazoefficiens lineage. The transformation of the model bacterium B. 
      diazoefficiens USDA110 with BjAC from E109 significantly increased its ability to 
      colonize soybean roots, experimentally recapitulating the beneficial effects of 
      the occurrence of BjAC in B. japonicum. In addition, the glxA mutation 
      significantly increased the nodulation competitiveness and plant growth-promoting 
      efficiency of E109. Finally, the potential applications of these types of 
      non-genetically modified mutant microbes in soybean production worldwide are 
      discussed.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Liebrenz, Karen
AU  - Liebrenz K
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina.
FAU - Gomez, Cristina
AU  - Gomez C
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina.
FAU - Brambilla, Silvina
AU  - Brambilla S
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina.
FAU - Frare, Romina
AU  - Frare R
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina.
FAU - Stritzler, Margarita
AU  - Stritzler M
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina.
FAU - Maguire, Vanina
AU  - Maguire V
AD  - Instituto Tecnologico Chascomus (INTECH-CONICET), Buenos Aires, Argentina.
FAU - Ruiz, Oscar
AU  - Ruiz O
AD  - Instituto Tecnologico Chascomus (INTECH-CONICET), Buenos Aires, Argentina.
FAU - Soldini, Diego
AU  - Soldini D
AD  - Estacion Experimental Agropecuaria Marcos Juarez, INTA, Cordoba, Argentina.
FAU - Pascuan, Cecilia
AU  - Pascuan C
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina.
FAU - Soto, Gabriela
AU  - Soto G
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina.
FAU - Ayub, Nicolas
AU  - Ayub N
AD  - Instituto de Agrobiotecnologia Y Biologia Molecular (INTA-CONICET), Buenos Aires, 
      Argentina. nicoayub@gmail.com.
AD  - Instituto de Genetica (IGEAF), INTA, De los Reseros S/N, Castelar C25(1712), 
      Buenos Aires, Argentina. nicoayub@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20211115
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (Antioxidants)
RN  - Bradyrhizobium japonicum
SB  - IM
MH  - *Glycine max/microbiology
MH  - Antioxidants/metabolism
MH  - Phylogeny
MH  - *Bradyrhizobium/genetics/metabolism
MH  - Symbiosis
MH  - Oxidative Stress
OTO - NOTNLM
OT  - Bradyrhizobium
OT  - Commercial inoculants
OT  - Evolution
OT  - Oxidative stress
OT  - Soybean
EDAT- 2021/11/17 06:00
MHDA- 2022/12/16 06:00
CRDT- 2021/11/16 06:53
PHST- 2021/09/17 00:00 [received]
PHST- 2021/11/09 00:00 [accepted]
PHST- 2021/11/17 06:00 [pubmed]
PHST- 2022/12/16 06:00 [medline]
PHST- 2021/11/16 06:53 [entrez]
AID - 10.1007/s00248-021-01925-2 [pii]
AID - 10.1007/s00248-021-01925-2 [doi]
PST - ppublish
SO  - Microb Ecol. 2022 Nov;84(4):1133-1140. doi: 10.1007/s00248-021-01925-2. Epub 2021 
      Nov 15.