PMID- 34786048
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211118
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 10
DP  - 2021
TI  - LncRNA SNHG4 promotes malignant biological behaviors and immune escape of 
      colorectal cancer cells by regulating the miR-144-3p/MET axis.
PG  - 11144-11161
AB  - OBJECTIVE: This study aimed to explore the underlying mechanism of long noncoding 
      RNA (lncRNA) SNHG4 regulating MET to participate in the malignant biologic 
      behaviors and immune escape of colorectal cancer (CRC) by sponging miR-144-3p. 
      METHODS: CRC tissues were collected and the expression levels of lncRNA SNHG4, 
      miR-144-3p, and MET were detected by quantitative real-time PCR (qRT-PCR). Then, 
      the localization of lncRNA SNHG4 was studied by fluorescence in situ 
      hybridization (FISH), and the regulatory relationship among lncRNA SNHG4, 
      miR-144-3p, and MET was verified by dual-luciferase reporter assay. Next, cell 
      counting kit-8 (CCK-8), Clone formation assay, and Transwell migration assay were 
      carried out to evaluate cell proliferation, colony formation, and invasion, 
      respectively. Flow cytometry was performed to evaluate cell apoptosis. Western 
      blotting was applied to semi-quantify the expression levels of MET and PD-L1 in 
      cells. RESULTS: LncRNA SNHG4 expression was upregulated in CRC tissues. Knockdown 
      of lncRNA SNHG4 suppressed the proliferation, colony formation and invasion of 
      CRC cells (all P<0.05). LncRNA SNHG4 directly regulated miR-144-3p, by which 
      either lncRNA SNHG4 knockdown or miR-144-3p overexpression can inhibit CD4+ T 
      cell apoptosis (both P<0.05) to suppress immune escape. Either overexpression of 
      lncRNA SNHG4 or knockdown of miR-144-3p activated PD-1/PD-L1 and induced CD4+ T 
      cell apoptosis (both P<0.05). LncRNA SNHG4 targeted and regulated MET through the 
      regulation of miR-144-3p, while overexpression of MET can partially reverse the 
      effect of lncRNA SNHG4 knockdown on CD4+ T cells. CONCLUSION: LncRNA SNHG4 
      sponges miR-144-3p and upregulates MET to promote the proliferation, colony 
      formation, invasion, and immune escape of CRC cells, leading to the progression 
      of CRC.
CI  - AJTR Copyright (c) 2021.
FAU - Zhou, Ning
AU  - Zhou N
AD  - Department of Pathology, Sichuan Mianyang 404 Hospital Mianyang 621000, Sichuan 
      Province, China.
FAU - Chen, Ying
AU  - Chen Y
AD  - Department of Pathology, Guiqian International General Hospital Guiyang 550000, 
      Guizhou Province, China.
FAU - Yang, Li
AU  - Yang L
AD  - Department of Pathology, The People's Hospital of Santai County Mianyang 621000, 
      Sichuan Province, China.
FAU - Xu, Tingting
AU  - Xu T
AD  - Department of Pathology, Sichuan Mianyang 404 Hospital Mianyang 621000, Sichuan 
      Province, China.
FAU - Wang, Fanrong
AU  - Wang F
AD  - Department of Pathology, Sichuan Mianyang 404 Hospital Mianyang 621000, Sichuan 
      Province, China.
FAU - Chen, Liqiao
AU  - Chen L
AD  - Department of Pathology, Sichuan Mianyang 404 Hospital Mianyang 621000, Sichuan 
      Province, China.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of General Surgery, Sichuan Mianyang 404 Hospital Mianyang 621000, 
      Sichuan Province, China.
FAU - Liu, Guangguo
AU  - Liu G
AD  - Department of Oncology, Sichuan Mianyang 404 Hospital Mianyang 621000, Sichuan 
      Province, China.
LA  - eng
PT  - Journal Article
DEP - 20211015
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8581836
OTO - NOTNLM
OT  - LncRNA SNHG4
OT  - MET
OT  - colorectal cancer
OT  - immune escape
OT  - miR-144-3p
COIS- None.
EDAT- 2021/11/18 06:00
MHDA- 2021/11/18 06:01
PMCR- 2021/10/15
CRDT- 2021/11/17 06:54
PHST- 2021/04/28 00:00 [received]
PHST- 2021/07/29 00:00 [accepted]
PHST- 2021/11/17 06:54 [entrez]
PHST- 2021/11/18 06:00 [pubmed]
PHST- 2021/11/18 06:01 [medline]
PHST- 2021/10/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 Oct 15;13(10):11144-11161. eCollection 2021.