PMID- 34786062
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211118
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 10
DP  - 2021
TI  - Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and 
      airway smooth muscle cell proliferation in mice with allergic asthma via 
      miR-486-5p/AQP5 axis.
PG  - 11341-11352
AB  - OBJECTIVE: This study aimed to investigate the effects of Wuling San and Xiao 
      Chaihu Decoction on allergic asthma, and elucidate the potential mechanism of 
      Wuling San and Xiao Chaihu Decoction for ameliorating allergic asthma. METHODS: 
      BALB/c mice were intraperitoneally injected with ovalbumin (OVA) to establish 
      animal model of allergic asthma. Transforming growth factor beta 1 (TGF-beta1) was 
      used to induce the proliferation of airway smooth muscle cells (ASMCs) in order 
      to establish the cell model. Quantitative real-time polymerase chain reaction 
      (qRT-PCR) was applied to quantify the expression levels of miR-486-5p and 
      aquaporin-5 (AQP5) in cells and tissues. Dual-luciferase reporter assay was used 
      to verify the targeting relationship between miR-486-5p and AQP5. MTT assay and 
      flow cytometry were carried out to evaluate cell proliferation and apoptosis, 
      respectively. Enzyme-linked immunosorbent assay (ELISA) was conducted to measure 
      the levels of interleukin-4 (IL-4), IL-5 and IL-13 in the bronchoalveolar lavage 
      fluid (BALF). Hematoxylin and eosin (HE) staining and Masson staining were used 
      to detect the recruitment of eosinophils and collagen deposition. RESULTS: In 
      both in vivo and in vitro experiments, Wuling San and Xiao Chaihu Decoction 
      significantly reduced the number of eosinophils, the levels of inflammatory 
      factors in the BALF of asthmatic mice, and the deposition of collagen in lung 
      tissues, and they also significantly inhibited the proliferation of ASMCs and 
      accelerated their apoptosis (all P<0.05). Wuling San and Xiao Chaihu Decoction 
      significantly upregulated the expression of AQP5 while inhibited the expression 
      of miR-486-5p; additionally, miR-486-5p negatively regulated the expression of 
      AQP5 (all P<0.05). Overexpression of miR-486-5p or silencing AQP5 can partially 
      reverse the therapeutic effect of Wuling San and Xiao Chaihu Decoction on 
      allergic asthma in mice and the inhibitory effect on the abnormal proliferation 
      of ASMCs (all P<0.05). CONCLUSION: Wuling San and Xiao Chaihu Decoction can 
      influence the proliferation and apoptosis of ASMCs and the expression of 
      inflammatory factors in mice with allergic asthma through inhibiting the 
      expression of miR-486-5p and upregulating the expression of AQP5.
CI  - AJTR Copyright (c) 2021.
FAU - Xue, Xirui
AU  - Xue X
AD  - Northwest Minzu University Lanzhou 730000, Gansu Province, China.
FAU - Liu, Xiaoping
AU  - Liu X
AD  - Northwest Minzu University Lanzhou 730000, Gansu Province, China.
FAU - Wei, Suzhen
AU  - Wei S
AD  - Northwest Minzu University Lanzhou 730000, Gansu Province, China.
FAU - Wang, Xin
AU  - Wang X
AD  - Northwest Minzu University Lanzhou 730000, Gansu Province, China.
FAU - Yang, Yanxia
AU  - Yang Y
AD  - Northwest Minzu University Lanzhou 730000, Gansu Province, China.
LA  - eng
PT  - Journal Article
DEP - 20211015
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8581894
OTO - NOTNLM
OT  - Wuling San and Xiao Chaihu Decoction
OT  - airway smooth muscle cells
OT  - aquaporin-5
OT  - inflammatory response
OT  - miR-486-5p
COIS- None.
EDAT- 2021/11/18 06:00
MHDA- 2021/11/18 06:01
PMCR- 2021/10/15
CRDT- 2021/11/17 06:54
PHST- 2021/04/15 00:00 [received]
PHST- 2021/08/13 00:00 [accepted]
PHST- 2021/11/17 06:54 [entrez]
PHST- 2021/11/18 06:00 [pubmed]
PHST- 2021/11/18 06:01 [medline]
PHST- 2021/10/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 Oct 15;13(10):11341-11352. eCollection 2021.