PMID- 34787641
OWN - NLM
STAT- MEDLINE
DCOM- 20211210
LR  - 20240404
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 62
IP  - 14
DP  - 2021 Nov 1
TI  - Long Noncoding RNA 3632454L22Rik Contributes to Corneal Epithelial Wound Healing 
      by Sponging miR-181a-5p in Diabetic Mice.
PG  - 16
LID - 10.1167/iovs.62.14.16 [doi]
LID - 16
AB  - PURPOSE: This work explores the abnormal expression of long noncoding RNAs 
      (lncRNAs), microRNAs (miRNAs) and messenger RNAs (mRNAs) in diabetic corneal 
      epithelial cells (CECs) and constructs an associated competitive endogenous RNA 
      (ceRNA) network. Moreover, we revealed that Rik may exert advantageous effects on 
      diabetic corneal epithelial wound closure by sponging miR-181a-5p. METHODS: We 
      obtained the profiles of differentially expressed lncRNAs (DELs) of CECs of type 
      1 diabetic versus control corneas by microarray and summarized the differentially 
      expressed miRNAs (DEmiRs) and differentially expressed genes (DEGs) data by 
      published literature. Subsequently, the ceRNA network was constructed using 
      bioinformatics analyses. The levels of lncRNA ENSMUST00000153610/3632454L22Rik 
      (Rik) and miR-181a-5p were verified. The localization of Rik was identified with 
      fluorescence in situ hybridization (FISH), and dual-luciferase assays proved the 
      targeted relationship between Rik and miR-181a-5p. Furthermore, we validated the 
      functional impact of Rik in vitro. RESULTS: Overall, 111 upregulated and 117 
      downregulated DELs were detected in diabetic versus control CECs. The level of 
      Rik located in both the cytoplasm and the nucleus was clearly downregulated, 
      whereas miR-181a-5p was upregulated in vitro and in vivo in the diabetic group 
      versus the control group. Rik can act as a ceRNA to bind to miR-181a-5p, thus 
      promoting diabetic corneal epithelial wound healing in vitro. CONCLUSIONS: This 
      work investigated the expression profile of DELs and constructed ceRNA networks 
      of diabetic CECs for the first time. Furthermore, we revealed that Rik may 
      positively impact diabetic corneal epithelial wound healing by sponging 
      miR-181a-5p, providing a novel potential therapeutic target of diabetic 
      keratopathy (DK).
FAU - Chen, Xiaxue
AU  - Chen X
AD  - Department of Ophthalmology, Fujian Medical University Union Hospital, Fu Zhou, 
      China.
FAU - Hu, Jianzhang
AU  - Hu J
AD  - Department of Ophthalmology, Fujian Medical University Union Hospital, Fu Zhou, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Blood Glucose)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (mirn181 microRNA, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Cell Line
MH  - Diabetes Mellitus, Experimental/*physiopathology
MH  - Diabetes Mellitus, Type 1/physiopathology
MH  - Epithelium, Corneal/*physiology
MH  - Gene Expression Regulation/*physiology
MH  - Gene Regulatory Networks
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics
MH  - Protein Array Analysis
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - RNA, Long Noncoding/*genetics
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Transfection
MH  - Up-Regulation
MH  - Wound Healing/*physiology
PMC - PMC8606839
COIS- Disclosure: X. Chen, None; J. Hu, None
EDAT- 2021/11/18 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/11/17
CRDT- 2021/11/17 12:23
PHST- 2021/11/17 12:23 [entrez]
PHST- 2021/11/18 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/11/17 00:00 [pmc-release]
AID - 2778087 [pii]
AID - IOVS-21-33486 [pii]
AID - 10.1167/iovs.62.14.16 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2021 Nov 1;62(14):16. doi: 10.1167/iovs.62.14.16.