PMID- 34787949
OWN - NLM
STAT- MEDLINE
DCOM- 20220223
LR  - 20220223
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 132
IP  - 3
DP  - 2022 Mar
TI  - Biochemical features and early adhesion of marine Candida parapsilosis strains on 
      high-density polyethylene.
PG  - 1954-1966
LID - 10.1111/jam.15369 [doi]
AB  - AIMS: Plastic debris are constantly released into oceans where, due to weathering 
      processes, they suffer fragmentation into micro- and nanoplastics. Diverse 
      microbes often colonize these persisting fragments, contributing to their 
      degradation. However, there are scarce reports regarding the biofilm formation of 
      eukaryotic decomposing microorganisms on plastics. Here, we evaluated five yeast 
      isolates from deep-sea sediment for catabolic properties and early adhesion 
      ability on high-density polyethylene (HDPE). METHODS AND RESULTS: We assessed 
      yeast catabolic features and adhesion ability on HDPE fragments subjected to 
      abiotic weathering. Adhered cells were evaluated through Crystal Violet Assay, 
      Scanning Electron Microscopy, Atomic Force Microscopy and Infrared Spectroscopy. 
      Isolates were identified as Candida parapsilosis and exhibited wide catabolic 
      capacity. Two isolates showed high adhesion ability on HDPE, consistently higher 
      than the reference C. parapsilosis strain, despite an increase in fragment 
      roughness due to weathering. Isolate Y5 displayed the most efficient 
      colonization, with production of polysaccharides and lipids after 48 h of 
      incubation. CONCLUSION: This work provides insights on catabolic metabolism and 
      initial yeast-HDPE interactions of marine C. parapsilosis strains. SIGNIFICANCE 
      AND IMPACT OF THE STUDY: Our findings represent an essential contribution to the 
      characterization of early interactions between deep-sea undescribed yeast strains 
      and plastic pollutants found in oceans.
CI  - (c) 2021 The Society for Applied Microbiology.
FAU - Oliveira, Maiara Monteiro
AU  - Oliveira MM
AUID- ORCID: 0000-0003-2574-9520
AD  - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical 
      Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
AD  - Immunology and Microbiology Laboratory, School of Health and Life Sciences, 
      Pontifical Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
FAU - Proenca, Audrey Menegaz
AU  - Proenca AM
AUID- ORCID: 0000-0002-3747-9719
AD  - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical 
      Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
AD  - Immunology and Microbiology Laboratory, School of Health and Life Sciences, 
      Pontifical Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
FAU - Moreira-Silva, Eduardo
AU  - Moreira-Silva E
AD  - Immunology and Microbiology Laboratory, School of Health and Life Sciences, 
      Pontifical Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
FAU - Dos Santos, Francine Melise
AU  - Dos Santos FM
AD  - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical 
      Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
FAU - Marconatto, Leticia
AU  - Marconatto L
AD  - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical 
      Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
FAU - de Castro, Aline Machado
AU  - de Castro AM
AD  - Biotechnology Division, Research and Development Center (CENPES), PETROBRAS, Rio 
      de Janeiro, RJ, Brazil.
FAU - Medina-Silva, Renata
AU  - Medina-Silva R
AUID- ORCID: 0000-0002-5466-4908
AD  - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical 
      Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
AD  - Immunology and Microbiology Laboratory, School of Health and Life Sciences, 
      Pontifical Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, Brazil.
LA  - eng
GR  - master s scholarship/Conselho Nacional de Desenvolvimento Cientifico e 
      Tecnologico/
GR  - master s scholarship (code 001)/Coordenacao de Aperfeicoamento de Pessoal de 
      Nivel Superior/
GR  - 0050.0096017.15.9/PETROBRAS/
PT  - Journal Article
DEP - 20211130
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Biofilms
MH  - *Candida parapsilosis/genetics/metabolism
MH  - Microscopy, Electron, Scanning
MH  - *Polyethylene/chemistry
OTO - NOTNLM
OT  - biofilms
OT  - degradation
OT  - diversity
OT  - metabolism
OT  - yeasts
EDAT- 2021/11/18 06:00
MHDA- 2022/02/24 06:00
CRDT- 2021/11/17 12:38
PHST- 2021/09/29 00:00 [revised]
PHST- 2021/05/08 00:00 [received]
PHST- 2021/10/14 00:00 [accepted]
PHST- 2021/11/18 06:00 [pubmed]
PHST- 2022/02/24 06:00 [medline]
PHST- 2021/11/17 12:38 [entrez]
AID - 10.1111/jam.15369 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2022 Mar;132(3):1954-1966. doi: 10.1111/jam.15369. Epub 2021 
      Nov 30.