PMID- 34790726
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220428
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 9
IP  - 20
DP  - 2021 Oct
TI  - MiR-34a promotes fibrosis of hepatic stellate cells via the TGF-beta pathway.
PG  - 1520
LID - 10.21037/atm-21-5005 [doi]
LID - 1520
AB  - BACKGROUND: Previous studies have confirmed that MicroRNA (miRNA) is a key 
      regulator exhibiting different effects in human liver fibrosis. However, the 
      function of miR-34a in liver fibrosis has not been reported. Hence, this study 
      aimed to investigate the regulatory mechanism of miR-34a in liver fibrosis. 
      METHODS: The expression of miR-34a was measured in fibrosis tissues via the 
      quantitative real-time PCR (qRT-PCR) assay. Subsequently, 30 male C57BL/6J mice 
      were divided into control and treatment groups and used to establish animal 
      models of liver fibrosis to explore the expression level of miR-34a. Moreover, 
      Cell Counting Kit 8 (CCK-8) and transwell assays were preformed to identify the 
      regulatory mechanism of miR-34a in cells. The effect of miR-34a on the activity 
      of transforming growth factor-beta (TGF-beta) pathway was observed by western blot. 
      RESULTS: Up-regulation of miR-34a was detected in fibrosis cells. Moreover, the 
      cellular phenotype was suppressed by miR-34a down-regulation in a primary culture 
      of hepatic stellate cells (HSCs). Besides, it was found that increased miR-34a 
      could significantly promote the invasion and migration of HSCs. Moreover, miR-34a 
      activates HSCs through transforming TGF-beta, alpha-smooth muscle actin (alpha-SMA), and 
      Monocyte chemoattractant protein-1 (MCP-1), which further affects liver fibrosis. 
      CONCLUSIONS: MiR-34a promotes the fibrosis of HSCs as well as cell proliferation, 
      migration, and invasion.
CI  - 2021 Annals of Translational Medicine. All rights reserved.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Department of Nutrition, Shandong Provincial Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan, China.
AD  - Department of Nutrition, Shandong Provincial Hospital Affiliated to Shandong 
      First Medical University, Jinan, China.
FAU - Wang, Haixia
AU  - Wang H
AD  - Healthcare Department, The Third Hospital of Jinan, Jinan, China.
FAU - Yao, Linlin
AU  - Yao L
AD  - Department of Cardiology, Shandong Provincial Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan, China.
AD  - Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong 
      First Medical University, Jinan, China.
FAU - Zhao, Peng
AU  - Zhao P
AD  - Department of Cardiology, Shandong Provincial Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan, China.
AD  - Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong 
      First Medical University, Jinan, China.
FAU - Wu, Xiaoyan
AU  - Wu X
AD  - Department of Cardiology, Shandong Provincial Hospital, Cheeloo College of 
      Medicine, Shandong University, Jinan, China.
AD  - Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong 
      First Medical University, Jinan, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8576652
OTO - NOTNLM
OT  - Bifico
OT  - MiR-34a
OT  - hepatic stellate cells (HSCs)
OT  - liver fibrosis
OT  - transforming growth factor-beta (TGF-beta)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at https://dx.doi.org/10.21037/atm-21-5005). The authors have no 
      conflicts of interest to declare.
EDAT- 2021/11/19 06:00
MHDA- 2021/11/19 06:01
PMCR- 2021/10/01
CRDT- 2021/11/18 07:02
PHST- 2021/07/07 00:00 [received]
PHST- 2021/10/19 00:00 [accepted]
PHST- 2021/11/18 07:02 [entrez]
PHST- 2021/11/19 06:00 [pubmed]
PHST- 2021/11/19 06:01 [medline]
PHST- 2021/10/01 00:00 [pmc-release]
AID - atm-09-20-1520 [pii]
AID - 10.21037/atm-21-5005 [doi]
PST - ppublish
SO  - Ann Transl Med. 2021 Oct;9(20):1520. doi: 10.21037/atm-21-5005.