PMID- 34790754
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220428
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 9
IP  - 20
DP  - 2021 Oct
TI  - Anticancer activity of ursolic acid on retinoblastoma cells determined by 
      bioinformatics analysis and validation.
PG  - 1548
LID - 10.21037/atm-21-4617 [doi]
LID - 1548
AB  - BACKGROUND: This article aims to explore whether ursolic acid (UA) inhibits the 
      progression of retinoblastoma (Rb) by regulating stearoyl-CoA desaturase (SCD). 
      METHODS: The Gene Expression Omnibus (GEO) database was used to filter the chip, 
      then the GEO2R software was used to analyze the microarray data (GSE97508, 
      GSE24673, and GSE110811). Gene set enrichment analysis (GSEA) was used to analyze 
      the relationship between the expression level of SCD and the proliferation, 
      migration, invasion, and inflammation in Rb patients. SO-RB50 and Y79 cell 
      proliferation, migration, and invasion were assessed by the CCK-8 assay, the 
      colony formation assay, the Transwell assay, and the wound scratch test. The 
      protein expression levels of SCD were measured by western blot. The mRNA 
      expression levels of IL-8, IL-6, CXCL1, and CCL2 were measured by RT-qPCR. The 
      protein expression levels of IL-8 and IL-6 were measured by ELISA. A xenograft 
      nude mouse model was established to evaluate the effect of UA on tumor growth in 
      male BALB/c mice. RESULTS: The expression levels of SCD were related to cell 
      proliferation, migration, invasion, and inflammation. UA inhibited SO-RB50 and 
      Y79 cell proliferation, migration, and invasion. At the same time, UA suppressed 
      tumor growth in the xenograft nude mouse model. Overexpression of SCD promoted 
      SO-RB50 and Y79 cell proliferation, migration, invasion, and inflammation, while 
      SCD knockout inhibited SO-RB50 and Y79 cell proliferation, migration, invasion, 
      and inflammation. Importantly, UA inhibited the proliferation, migration, and 
      invasion of Rb cells through SCD inhibition. CONCLUSIONS: UA inhibited the 
      proliferation, migration, and invasion of Rb cells through SCD. This provides a 
      new scientific basis for targeted therapy of Rb.
CI  - 2021 Annals of Translational Medicine. All rights reserved.
FAU - Zhou, Dan
AU  - Zhou D
AD  - Department of Ophthalmology, Suzhou High Tech Zone People's Hospital, Suzhou, 
      China.
FAU - Bao, Qi
AU  - Bao Q
AD  - Department of Ophthalmology, Suzhou High Tech Zone People's Hospital, Suzhou, 
      China.
FAU - Fu, Songbin
AU  - Fu S
AD  - Department of Ophthalmology, Harbin Medical University, Harbin, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8576664
OTO - NOTNLM
OT  - Retinoblastoma (Rb)
OT  - inflammation
OT  - invasion
OT  - migration
OT  - proliferation
OT  - stearoyl-CoA desaturase (SCD)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at https://dx.doi.org/10.21037/atm-21-4617). The authors have no 
      conflicts of interest to declare.
EDAT- 2021/11/19 06:00
MHDA- 2021/11/19 06:01
PMCR- 2021/10/01
CRDT- 2021/11/18 07:02
PHST- 2021/08/17 00:00 [received]
PHST- 2021/09/28 00:00 [accepted]
PHST- 2021/11/18 07:02 [entrez]
PHST- 2021/11/19 06:00 [pubmed]
PHST- 2021/11/19 06:01 [medline]
PHST- 2021/10/01 00:00 [pmc-release]
AID - atm-09-20-1548 [pii]
AID - 10.21037/atm-21-4617 [doi]
PST - ppublish
SO  - Ann Transl Med. 2021 Oct;9(20):1548. doi: 10.21037/atm-21-4617.