PMID- 34790780 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220428 IS - 2305-5839 (Print) IS - 2305-5847 (Electronic) IS - 2305-5839 (Linking) VI - 9 IP - 20 DP - 2021 Oct TI - Changes in aortic collagen in beta-aminopropionitrile-induced acute aortic dissection. PG - 1574 LID - 10.21037/atm-21-4933 [doi] LID - 1574 AB - BACKGROUND: The precise role collagen plays in acute aortic dissection (AAD) was investigated in an animal model of beta-aminopropinitrile (BAPN)-induced AAD. METHODS: The 30 3-week-old male specific-pathogen free (SPF)-grade Sprague-Dawley (SD) rats were randomly divided into two groups: 10 in the Control group and 20 in the Model group. The Model group was treated with 0.1% BAPN for 4 weeks, while the Control group received untreated water. Histopathological staining and western blot were used to detect changes of the extracellular matrix (ECM) and collagen content in the aorta. RESULTS: At the end of the experiment, the incidence of AAD was 25%, the aortic ECM of surviving rats was severely damaged, and the arrangement was disordered. Fibroblast cells are unevenly distributed, with wide gaps, collagen fibers were also distributed unevenly in a disordered arrangement and their thickness was uneven. The elastic membrane disappeared over a large area. Compare to Control group, the Collagen types I, III and their subunits were upregulated (P<0.05), while matrix metalloproteinase (MMP) 2 and MMP9 were downregulated in the aorta of Model group (P<0.05). CONCLUSIONS: In the animal model of BAPN-induced AAD, collagen types I, III and subunits were increased, while MMP2 and MMP9 were decreased in thoracic aorta, which may lead to stiffness of the aorta and be the cause of dissection. CI - 2021 Annals of Translational Medicine. All rights reserved. FAU - Zhou, Hao AU - Zhou H AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. FAU - Ren, Yan AU - Ren Y AD - Department of Cardiac Surgery, Zunyi Medical University Affiliated Hospital, Zunyi, China. FAU - Xiao, Jun AU - Xiao J AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. FAU - He, Jian AU - He J AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. FAU - Zhang, Yuling AU - Zhang Y AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. FAU - Qiu, Zhihuang AU - Qiu Z AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. FAU - Huang, Qiuyu AU - Huang Q AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. FAU - Hu, Yunnan AU - Hu Y AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. FAU - Chen, Liangwan AU - Chen L AD - Department of Cardiac Surgery, Fujian Medical University Union Hospital, Fuzhou, China. AD - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. AD - Fujian Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fuzhou, China. LA - eng PT - Journal Article PL - China TA - Ann Transl Med JT - Annals of translational medicine JID - 101617978 PMC - PMC8576682 OTO - NOTNLM OT - Acute aortic dissection (AAD) OT - animal models OT - collagen OT - matrix metalloproteinase OT - beta-aminopropinitrile (BAPN) COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://dx.doi.org/10.21037/atm-21-4933). The authors have no conflicts of interest to declare. EDAT- 2021/11/19 06:00 MHDA- 2021/11/19 06:01 PMCR- 2021/10/01 CRDT- 2021/11/18 07:02 PHST- 2021/09/01 00:00 [received] PHST- 2021/10/16 00:00 [accepted] PHST- 2021/11/18 07:02 [entrez] PHST- 2021/11/19 06:00 [pubmed] PHST- 2021/11/19 06:01 [medline] PHST- 2021/10/01 00:00 [pmc-release] AID - atm-09-20-1574 [pii] AID - 10.21037/atm-21-4933 [doi] PST - ppublish SO - Ann Transl Med. 2021 Oct;9(20):1574. doi: 10.21037/atm-21-4933.