PMID- 34792197
OWN - NLM
STAT- MEDLINE
DCOM- 20220124
LR  - 20220124
IS  - 1745-4514 (Electronic)
IS  - 0145-8884 (Linking)
VI  - 45
IP  - 12
DP  - 2021 Dec
TI  - Crocetin attenuating Urinary tract Infection and adherence of uropathogenic E. 
      coli in NRK-52E cells via an inflammatory pathway.
PG  - e13998
LID - 10.1111/jfbc.13998 [doi]
AB  - BACKGROUND AND OBJECTIVE: Urinary tract infections (UTI) are commonly treated 
      with broad-spectrum antibiotics, but treatment has limitation due to causes of 
      nephrotoxicity in uroepithelial cells. Recently, the researcher focuses their 
      research on alternative therapy for the treatment of UTI. This study evaluated 
      the anti-infectious effect of crocetin against adherence of pathogenic [2-(14) 
      C]-acetate labeled Escherichia coli (MTCC-729) to rat proximal renal tubular 
      cells (NRK-52E cells) and explores the possible mechanism of action. MATERIALS 
      AND METHODS: In vitro cytotoxicity and radio acetate labeled tests were performed 
      on NRK-52E cells. The rats were divided into five different groups as follows: 
      normal control (NC), disease control (DC), and various doses of crocetin (1.25, 
      2.5, and 5 mg/kg) treated group rats. White blood cells in blood, urine, and 
      bacterial colony counts were estimated at regular intervals. Pro-inflammatory 
      cytokines, such as interleukin-6 (IL-6), monocyte chemoattractant protein-1 
      (MCP-1), interleukin-10 (IL-10), and interleukin-8 (IL-8), were also estimated. 
      In the current study, we estimated the mRNA expression of toll-like receptor-4 
      (TLR-4) and toll-like receptor-2 (TLR-2) in the renal and bladder tissues. 
      RESULTS: Crocetin significantly (p < .05) inhibited the adherence of E. Coli in 
      NRK-52E cells. Crocetin suppresses the lipid peroxidation (LPO) 42% in cells 
      treated with H(2) O(2) cells without crocetin. The white blood cells (WBC) count 
      in blood and urine were augmented and crocetin treatment significantly (p < .05) 
      reduced the WBC in urine and blood. The pro-inflammatory cytokines, such as IL-6, 
      MCP-1, IL-10, and IL-8, significantly (p < .05) increased in the DC group and 
      crocetin significantly (p < .05) reduced the pro-inflammatory cytokines. 
      Dose-dependent treatment of crocetin significantly reduced the mRNA expression of 
      TLR2 and TLR4 in the renal and bladder tissues. CONCLUSION: Crocetin considerably 
      reduced the bacterial adherence to NRK-52E cells, attenuated the H(2) O(2) 
      induced toxicity in NRK-52E cells and also improved the renal tubular function, 
      and reduced the inflammatory response via altering the inflammatory and 
      antioxidant markers. PRACTICAL APPLICATION: As we all know that urinary tract 
      infection is the most common disease worldwide. In this study, we scrutinized the 
      protective effect of crocetin against urinary tract infection. Crocetin treatment 
      considerably reduced the zone of inhibition and improved radioactivity. Crocetin 
      significantly reduced the levels of cytokines and inflammatory mediators. 
      Crocetin can be used as a protective drug in the treatment of urinary tract 
      infections.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Yang, Jian
AU  - Yang J
AD  - Department of Nephrology, Baoji Hospital of traditional Chinese Medicine, Baoji, 
      China.
FAU - Qiu, Xin
AU  - Qiu X
AD  - Department of Nephrology, Baoji Hospital of traditional Chinese Medicine, Baoji, 
      China.
FAU - Zhou, Meilan
AU  - Zhou M
AUID- ORCID: 0000-0002-9757-6246
AD  - Department of Nephrology, Xijing Hospital, The First Affiliated Hospital of 
      Fourth Military Medical University, Xi'an, China.
FAU - Wang, Di
AU  - Wang D
AUID- ORCID: 0000-0002-2800-9803
AD  - Department of Nephrology, Xijing Hospital, The First Affiliated Hospital of 
      Fourth Military Medical University, Xi'an, China.
LA  - eng
PT  - Journal Article
DEP - 20211118
PL  - United States
TA  - J Food Biochem
JT  - Journal of food biochemistry
JID - 7706045
RN  - 0 (trans-sodium crocetinate)
RN  - 11103-57-4 (Vitamin A)
RN  - 36-88-4 (Carotenoids)
SB  - IM
MH  - Animals
MH  - Carotenoids/pharmacology
MH  - Rats
MH  - *Urinary Tract Infections/drug therapy
MH  - *Uropathogenic Escherichia coli
MH  - Vitamin A/analogs & derivatives
OTO - NOTNLM
OT  - Escherichia coli
OT  - NRK-52E cells
OT  - crocetin
OT  - pro-inflammatory cytokines
EDAT- 2021/11/19 06:00
MHDA- 2022/01/27 06:00
CRDT- 2021/11/18 08:55
PHST- 2021/09/17 00:00 [revised]
PHST- 2021/06/12 00:00 [received]
PHST- 2021/09/19 00:00 [accepted]
PHST- 2021/11/19 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/11/18 08:55 [entrez]
AID - 10.1111/jfbc.13998 [doi]
PST - ppublish
SO  - J Food Biochem. 2021 Dec;45(12):e13998. doi: 10.1111/jfbc.13998. Epub 2021 Nov 
      18.