PMID- 34792426
OWN - NLM
STAT- MEDLINE
DCOM- 20220310
LR  - 20220310
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 40
IP  - 12_suppl
DP  - 2021 Dec
TI  - Tanshinone IIA improves degranulation of mast cells and allergic rhinitis induced 
      by ovalbumin by inhibiting the PLCgamma1/PKC/IP3R pathway.
PG  - S702-S710
LID - 10.1177/09603271211058884 [doi]
AB  - Allergic rhinitis (AR) is a common allergic inflammatory and chronic reactive 
      disease caused by allergen-induced immunoglobulin E (IgE). Tanshinone IIA (Tan 
      IIA) is one of the active ingredients in Salvia miltiorrhiza Bunge (Danshen) and 
      plays a vital role in inhibiting inflammation. Thus, we hypothesized that Tan IIA 
      has anti-allergic effects and studied the function of Tan IIA in mast cells and 
      an AR animal model. We induced RBL-2H3 cell sensitization with monoclonal 
      anti-2,4,6-dinitrophenyl-immunoglobulin (Ig) E/human serum albumin (DNP-IgE/HSA) 
      and constructed an ovalbumin (OVA)-induced AR model in mice. The role of Tan IIA 
      in AR progression was studied using the MTT assay, ELISA, western blot, toluidine 
      blue staining, HE staining, and Alcian blue and safranin O (A&S) staining. Tan 
      IIA treatment significantly increased IgE/HSA-induced cell viability. However, 
      Tan IIA treatment markedly downregulated the expression levels of 
      beta-hexosaminidase, histamine, tumor necrosis factor (TNF-alpha), interleukin 1beta 
      (IL-1beta), IL-4, and IL-5 in IgE/HSA-induced cells. Furthermore, Tan IIA improved 
      typical symptoms in the OVA-induced AR model mice by inhibiting the phospholipase 
      Cgamma1 (PLCgamma1)/protein kinase C (PKC)/IP3R pathway. Additionally, Tan IIA 
      effectively improved the degranulation of RBL-2H3 cells and OVA-induced AR in 
      mice. Together, these results suggest that Tan IIA may be a potential drug for 
      the treatment of AR in the future.
FAU - Li, Shouye
AU  - Li S
AD  - College of Pharmacy, 117839Hangzhou Medical College, Hangzhou, China.
FAU - Li, Zheming
AU  - Li Z
AD  - College of Pharmacy, 117839Hangzhou Medical College, Hangzhou, China.
FAU - Tan, Tao
AU  - Tan T
AD  - Internal Medicine Department, Zhejiang Provincial General Team Hospital of the 
      Chinese People's Armed Police Force, Hangzhou, China.
FAU - Dai, Shijie
AU  - Dai S
AD  - College of Pharmacy, 117839Hangzhou Medical College, Hangzhou, China.
FAU - Wu, Yangsheng
AU  - Wu Y
AD  - College of Pharmacy, 117839Hangzhou Medical College, Hangzhou, China.
FAU - Xu, Faying
AU  - Xu F
AUID- ORCID: 0000-0003-0716-2312
AD  - School of Medical Imaging, 117839Hangzhou Medical College, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20211118
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Abietanes)
RN  - 03UUH3J385 (tanshinone)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.4.11 (PLCG1 protein, human)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Abietanes/*pharmacology/therapeutic use
MH  - Animals
MH  - Cell Degranulation/*drug effects
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Mast Cells/*drug effects
MH  - Mice
MH  - Ovalbumin/adverse effects
MH  - Phospholipase C gamma/*metabolism
MH  - Protein Kinase C/*metabolism
MH  - Rhinitis, Allergic/*drug therapy/metabolism
OTO - NOTNLM
OT  - Tanshinone IIA
OT  - allergic rhinitis
OT  - degranulation
OT  - mast cell
OT  - ovalbumin
EDAT- 2021/11/19 06:00
MHDA- 2022/03/11 06:00
CRDT- 2021/11/18 12:16
PHST- 2021/11/19 06:00 [pubmed]
PHST- 2022/03/11 06:00 [medline]
PHST- 2021/11/18 12:16 [entrez]
AID - 10.1177/09603271211058884 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2021 Dec;40(12_suppl):S702-S710. doi: 10.1177/09603271211058884. 
      Epub 2021 Nov 18.