PMID- 34792748 OWN - NLM STAT- MEDLINE DCOM- 20220202 LR - 20220202 IS - 1559-0291 (Electronic) IS - 0273-2289 (Linking) VI - 194 IP - 1 DP - 2022 Jan TI - Therapeutic Potential of Cucumis melo (L.) Fruit Extract and Its Silver Nanopartciles Against DEN-Induced Hepatocellular Cancer in Rats. PG - 368-381 LID - 10.1007/s12010-021-03765-9 [doi] AB - Biosynthesized silver nanoparticles have a wide range of biological activities and using nanoparticles as one of the novel approaches in cancer therapy. In this present research work, the anti-cancer efficacy of Cucumis melo fruit extract and its silver nanoparticles was explored. Wistar rats were divided into six groups and hepatic cancer was induced with 0.01% DEN (diethylnitrosamine) through drinking water for 16 weeks. Cyclophosphamide was given as the standard drug at the dose of 50 mg/kg body weight. Hematological parameters showed a decrease in the levels of hemoglobin (Hb), packed cell volume (PCV), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular Hb (MCH), mean corpuscular Hb concentration (MCHC), and platelets (PLTS) levels except white blood cell (WBC) in DEN-induced cancer animals. Significant alterations in the hematological parameters were observed after treatment which indicate the protective effect of Cucumis melo fruit on the hemopoietic system. The structural integrity of the cells has been damaged in cancer-induced animals, and this results in cytoplasmic leakage of enzyme into the blood stream, leads to the elevated levels of these enzymes in blood with subsequent fall in the tissues. Hence, the levels of liver function markers such as AST ALT, ALP, LDH, GGT, and 5'NT were significantly elevated in serum and the liver of cancer-induced rats. The levels of serum tumor markers, viz., alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), elevated in rats induced with DEN, which then were reduced following Cucumis melo fruit treatment, indicating the anti-cancer activity of the drug. Histological evaluation of the liver and kidney was also performed to authenticate the present work. Treatment with crude extract and silver nanoparticles of Cucumis melo fruit indicates that Cucumis melo fruit could have exerted its protective effect. CI - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Vidya, R AU - Vidya R AD - Department of Biochemistry, Vels Institute of Science, Technology & Advanced Studies, Chennai, Tamil Nadu, 600117, India. vidyabiochem@gmail.com. FAU - Kalaivani, K AU - Kalaivani K AD - Department of Biochemistry, Kongunadu Arts and Science College (Autonomous), Coimbatore, Tamil Nadu, 641029, India. FAU - Amudha, P AU - Amudha P AD - Department of Biochemistry, Vels Institute of Science, Technology & Advanced Studies, Chennai, Tamil Nadu, 600117, India. LA - eng PT - Journal Article DEP - 20211118 PL - United States TA - Appl Biochem Biotechnol JT - Applied biochemistry and biotechnology JID - 8208561 RN - 0 (Plant Extracts) RN - 3IQ78TTX1A (Diethylnitrosamine) RN - 3M4G523W1G (Silver) SB - IM MH - Animals MH - *Carcinoma, Hepatocellular/chemically induced/drug therapy/metabolism MH - Cucumis melo/*chemistry MH - Diethylnitrosamine/*toxicity MH - Fruit/*chemistry MH - *Liver Neoplasms, Experimental/chemically induced/drug therapy/metabolism MH - Male MH - *Metal Nanoparticles/chemistry/therapeutic use MH - Plant Extracts/*chemistry MH - Rats MH - Rats, Wistar MH - *Silver/chemistry/pharmacology OTO - NOTNLM OT - Cucumis melo (L) OT - Hematological profile OT - Liver function markers OT - Silver nanoparticles OT - Tumor markers EDAT- 2021/11/19 06:00 MHDA- 2022/02/03 06:00 CRDT- 2021/11/18 12:29 PHST- 2021/07/31 00:00 [received] PHST- 2021/11/08 00:00 [accepted] PHST- 2021/11/19 06:00 [pubmed] PHST- 2022/02/03 06:00 [medline] PHST- 2021/11/18 12:29 [entrez] AID - 10.1007/s12010-021-03765-9 [pii] AID - 10.1007/s12010-021-03765-9 [doi] PST - ppublish SO - Appl Biochem Biotechnol. 2022 Jan;194(1):368-381. doi: 10.1007/s12010-021-03765-9. Epub 2021 Nov 18.