PMID- 34793174
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220127
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 37
IP  - 48
DP  - 2021 Dec 7
TI  - A SiO(2) Microcarrier with an Opal-like Structure for Cross-Linked Enzyme 
      Immobilization.
PG  - 14147-14156
LID - 10.1021/acs.langmuir.1c02389 [doi]
AB  - The opal-like SiO(2) microcarriers with different pore diameters named 
      opal-SiO(2)I and opal-SiO(2)II were synthesized and utilized as microcarriers to 
      immobilize Rhizopus oryzae lipase (ROL) and Aspergillus oryzae alpha-amylases (AOA). 
      ROL and AOA can be more stably immobilized on the cross-linked SiO(2) opals by 
      neopentyl glycol diglycidyl ether (NGDE), which is the first attempt to use it as 
      a cross-linking agent compared with glutaraldehyde. According to the morphology 
      analysis, multiple layers of SiO(2) monodisperse microspheres were regularly 
      packed and formed an opal-like structure, and enzymes were well scattered and 
      immobilized throughout the SiO(2) opals. The results showed that the performance 
      of enzymes immobilized on opal-SiO(2)II with a larger specific surface area was 
      much better than that of opal-SiO(2)I. The enzyme activity of ROL@opal-SiO(2)II 
      and AOA@opal-SiO(2)II cross-linked with 1% NGDE increased 5.32 and 9.32 times 
      compared with their free counterpart, respectively. Furthermore, pH and thermal 
      stability and reusability of ROL/AOA@opal-SiO(2)II were significantly improved 
      and higher than those of ROL/AOA@opal-SiO(2)I and free enzymes. This study 
      provides an easily obtained microcarrier opal-SiO(2)II, which shows potential for 
      efficient different enzyme immobilizations and further industrial applications.
FAU - Luo, Yixian
AU  - Luo Y
AD  - Engineering Research Center of Industrial Microbiology of Ministry of Education, 
      College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
FAU - Jin, Dou
AU  - Jin D
AD  - Engineering Research Center of Industrial Microbiology of Ministry of Education, 
      College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
FAU - He, Wenjin
AU  - He W
AD  - Center of Engineering Technology Research for Microalgae Germplasm Improvement of 
      Fujian, Southern Institute of Oceanography, College of Life Sciences, Fujian 
      Normal University, Fuzhou, Fujian 350117, China.
FAU - Huang, Jianzhong
AU  - Huang J
AD  - Engineering Research Center of Industrial Microbiology of Ministry of Education, 
      College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
FAU - Chen, Aicheng
AU  - Chen A
AD  - Fujian Province University Engineering Research Center of Industrial 
      Biocatalysis, Fujian Provincial Key Laboratory of Advanced Materials Oriented 
      Chemical Engineering, College of Chemistry and Materials Science, Fujian Normal 
      University, Fuzhou, Fujian 350117, China.
FAU - Qi, Feng
AU  - Qi F
AUID- ORCID: 0000-0001-6349-179X
AD  - Engineering Research Center of Industrial Microbiology of Ministry of Education, 
      College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211118
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Enzymes, Immobilized)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Enzyme Stability
MH  - *Enzymes, Immobilized/metabolism
MH  - Glutaral
MH  - Hydrogen-Ion Concentration
MH  - Microspheres
MH  - *Silicon Dioxide
MH  - Temperature
EDAT- 2021/11/19 06:00
MHDA- 2022/01/28 06:00
CRDT- 2021/11/18 17:12
PHST- 2021/11/19 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2021/11/18 17:12 [entrez]
AID - 10.1021/acs.langmuir.1c02389 [doi]
PST - ppublish
SO  - Langmuir. 2021 Dec 7;37(48):14147-14156. doi: 10.1021/acs.langmuir.1c02389. Epub 
      2021 Nov 18.