PMID- 34793243
OWN - NLM
STAT- MEDLINE
DCOM- 20220202
LR  - 20230801
IS  - 2470-9468 (Electronic)
IS  - 2470-9468 (Linking)
VI  - 7
IP  - 67
DP  - 2022 Jan 21
TI  - Allelic variation in class I HLA determines CD8(+) T cell repertoire shape and 
      cross-reactive memory responses to SARS-CoV-2.
PG  - eabk3070
LID - 10.1126/sciimmunol.abk3070 [doi]
LID - eabk3070
AB  - Effective presentation of antigens by human leukocyte antigen (HLA) class I 
      molecules to CD8(+) T cells is required for viral elimination and generation of 
      long-term immunological memory. In this study, we applied a single-cell, 
      multiomic technology to generate a unified ex vivo characterization of the CD8(+) 
      T cell response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 
      across four major HLA class I alleles. We found that HLA genotype conditions key 
      features of epitope specificity, TCRalpha/beta sequence diversity, and the utilization 
      of pre-existing SARS-CoV-2-reactive memory T cell pools. Single-cell 
      transcriptomics revealed functionally diverse T cell phenotypes of 
      SARS-CoV-2-reactive T cells, associated with both disease stage and epitope 
      specificity. Our results show that HLA variations notably influence the CD8(+) T 
      cell repertoire shape and utilization of immune recall upon SARS-CoV-2 infection.
FAU - Francis, Joshua M
AU  - Francis JM
AUID- ORCID: 0000-0001-5727-7498
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Leistritz-Edwards, Del
AU  - Leistritz-Edwards D
AUID- ORCID: 0000-0002-5286-6573
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Dunn, Augustine
AU  - Dunn A
AUID- ORCID: 0000-0002-8683-1459
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Tarr, Christina
AU  - Tarr C
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Lehman, Jesse
AU  - Lehman J
AUID- ORCID: 0000-0002-3729-2866
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Dempsey, Conor
AU  - Dempsey C
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Hamel, Andrew
AU  - Hamel A
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Rayon, Violeta
AU  - Rayon V
AUID- ORCID: 0000-0002-2446-2395
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Liu, Gang
AU  - Liu G
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Wang, Yuntong
AU  - Wang Y
AUID- ORCID: 0000-0001-9331-2959
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Wille, Marcos
AU  - Wille M
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Durkin, Melissa
AU  - Durkin M
AUID- ORCID: 0000-0001-6886-2203
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Hadley, Kane
AU  - Hadley K
AUID- ORCID: 0000-0002-2520-155X
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Sheena, Aswathy
AU  - Sheena A
AUID- ORCID: 0000-0002-7277-457X
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Roscoe, Benjamin
AU  - Roscoe B
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Ng, Mark
AU  - Ng M
AUID- ORCID: 0000-0002-3289-135X
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Rockwell, Graham
AU  - Rockwell G
AUID- ORCID: 0000-0003-2738-326X
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Manto, Margaret
AU  - Manto M
AUID- ORCID: 0000-0002-9393-179X
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Gienger, Elizabeth
AU  - Gienger E
AUID- ORCID: 0000-0001-7647-1229
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Nickerson, Joshua
AU  - Nickerson J
AUID- ORCID: 0000-0001-5125-229X
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
CN  - MGH COVID-19 Collection and Processing Team
AD  - Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
FAU - Moarefi, Amir
AU  - Moarefi A
AUID- ORCID: 0000-0002-3047-8262
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Noble, Michael
AU  - Noble M
AUID- ORCID: 0000-0002-6704-1793
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Malia, Thomas
AU  - Malia T
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Bardwell, Philip D
AU  - Bardwell PD
AUID- ORCID: 0000-0002-3835-255X
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Gordon, William
AU  - Gordon W
AUID- ORCID: 0000-0003-2032-2325
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Swain, Joanna
AU  - Swain J
AUID- ORCID: 0000-0003-0818-8434
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Skoberne, Mojca
AU  - Skoberne M
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Sauer, Karsten
AU  - Sauer K
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Harris, Tim
AU  - Harris T
AUID- ORCID: 0000-0002-5621-7861
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Goldrath, Ananda W
AU  - Goldrath AW
AUID- ORCID: 0000-0002-5900-508X
AD  - Division of Biological Sciences, Molecular Biology Section, University of 
      California, San Diego, San Diego, CA, USA.
FAU - Shalek, Alex K
AU  - Shalek AK
AUID- ORCID: 0000-0001-5670-8778
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
AD  - Department of Chemistry and Koch Institute for Integrative Cancer Research, 
      Institute for Medical Engineering and Science, Massachusetts Institute of 
      Technology, Cambridge, MA, USA.
AD  - Broad Institute of MIT and Harvard, Cambridge, MA, USA.
AD  - Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
FAU - Coyle, Anthony J
AU  - Coyle AJ
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
FAU - Benoist, Christophe
AU  - Benoist C
AUID- ORCID: 0000-0003-1172-6555
AD  - Department of Immunology, Harvard Medical School, Boston, MA, USA.
FAU - Pregibon, Daniel C
AU  - Pregibon DC
AUID- ORCID: 0000-0003-1084-3653
AD  - Repertoire Immune Medicines, Cambridge, MA, USA.
LA  - eng
GR  - R37 AI067545/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220121
PL  - United States
TA  - Sci Immunol
JT  - Science immunology
JID - 101688624
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - *Alleles
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - *COVID-19/genetics/immunology
MH  - Histocompatibility Antigens Class I/genetics/*immunology
MH  - Humans
MH  - Memory T Cells/*immunology
MH  - *Receptors, Antigen, T-Cell, alpha-beta/genetics/immunology
MH  - SARS-CoV-2/genetics/*immunology
PMC - PMC9017864
EDAT- 2021/11/19 06:00
MHDA- 2022/02/03 06:00
PMCR- 2021/11/18
CRDT- 2021/11/18 17:13
PHST- 2021/11/19 06:00 [pubmed]
PHST- 2022/02/03 06:00 [medline]
PHST- 2021/11/18 17:13 [entrez]
PHST- 2021/11/18 00:00 [pmc-release]
AID - 10.1126/sciimmunol.abk3070 [pii]
AID - abk3070 [pii]
AID - 10.1126/sciimmunol.abk3070 [doi]
PST - ppublish
SO  - Sci Immunol. 2022 Jan 21;7(67):eabk3070. doi: 10.1126/sciimmunol.abk3070. Epub 
      2022 Jan 21.