PMID- 34796777
OWN - NLM
STAT- MEDLINE
DCOM- 20220330
LR  - 20220531
IS  - 1607-842X (Electronic)
IS  - 0891-6934 (Linking)
VI  - 55
IP  - 1
DP  - 2022 Feb
TI  - Circ-METTL15 contributes to the proliferation, metastasis, immune escape and 
      restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis.
PG  - 8-20
LID - 10.1080/08916934.2021.2001801 [doi]
AB  - BACKGROUND: Circular RNAs (circRNAs) are important regulators in the pathogenesis 
      of lung cancer. The study aims to explore the function and mechanism of circRNA 
      methyltransferase-like 15 (circ-METTL15) in lung cancer development. METHODS: The 
      expression of circ-METTL15, miR-1299 and programmed death-ligand 1 (PDL1) were 
      investigated by qRT-PCR assay. Cell viability, colony formation, cell 
      proliferation and invasion were determined by MTT, colony formation, EDU 
      incorporation and transwell assays, respectively. Cell apoptosis was attested by 
      flow cytometry and TUNEL assays. Interferon-gamma (IFN-gamma) and Tumour Necrosis 
      Factor-alpha (TNF-alpha) production were tested by enzyme-linked immunosorbent assay 
      (ELISA), and the survival rate of cancer cells was assessed by cytotoxicity 
      analysis. The protein expression was examined by western blot or 
      immunohistochemistry (IHC) assay. The interaction between miR-1299 and 
      circ-METTL15 or PDL1 was confirmed via dual-luciferase reporter assay. Xenograft 
      models were established in mice to explore the role of circ-METTL15 in tumour 
      growth in vivo. RESULTS: Circ-METTL15 was upregulated in lung cancer tissues and 
      cells. Circ-METTL15 silencing suppressed cell proliferation, colony formation, 
      invasion, immune escape and promoted cell apoptosis in lung cancer cells. 
      Circ-METTL15 was a sponge of miR-1299, and it could exert regulatory function in 
      lung cancer via miR-1299. Furthermore, PDL1 was a functional target of miR-1299, 
      and miR-1299 inhibited lung cancer cell development via decreasing PDL1 
      expression. Moreover, circ-METTL15 controlled PDL1 expression by acting as a 
      sponge of miR-1299. Besides, circ-METTL15 downregulation blocked lung cancer 
      tumour growth in vivo by regulating the miR-1299/PDL1 axis. CONCLUSION: 
      Circ-METTL15 promoted lung cancer malignant progression at least partly through 
      modulating PDL1 by sponging miR-1299.
FAU - Zhang, Rui
AU  - Zhang R
AD  - Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, 
      China.
FAU - Shang, Liang
AU  - Shang L
AD  - Department of Pharmacy, Jilin Provincial Cancer Hospital, Changchun, China.
FAU - Nan, Jinniang
AU  - Nan J
AD  - Department of Clinical Medicine, Jiangxi Health Vocational College of China, 
      Nanchang, China.
FAU - Niu, Kai
AU  - Niu K
AD  - Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, 
      China.
FAU - Dai, Jixin
AU  - Dai J
AD  - Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, 
      China.
FAU - Jin, Xintian
AU  - Jin X
AD  - Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, 
      China.
FAU - Zhang, Xianghua
AU  - Zhang X
AD  - Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20211119
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (B7-H1 Antigen)
RN  - 0 (CD274 protein, human)
RN  - 0 (MIRN1299 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - *B7-H1 Antigen/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Humans
MH  - *Lung Neoplasms/genetics
MH  - Mice
MH  - *MicroRNAs/genetics
MH  - RNA, Circular/*genetics
OTO - NOTNLM
OT  - *Lung cancer
OT  - *PDL1
OT  - *circ-METTL15
OT  - *immune escape
OT  - *miR-1299
EDAT- 2021/11/20 06:00
MHDA- 2022/03/31 06:00
CRDT- 2021/11/19 08:40
PHST- 2021/11/20 06:00 [pubmed]
PHST- 2022/03/31 06:00 [medline]
PHST- 2021/11/19 08:40 [entrez]
AID - 10.1080/08916934.2021.2001801 [doi]
PST - ppublish
SO  - Autoimmunity. 2022 Feb;55(1):8-20. doi: 10.1080/08916934.2021.2001801. Epub 2021 
      Nov 19.