PMID- 34798782
OWN - NLM
STAT- MEDLINE
DCOM- 20220204
LR  - 20220204
IS  - 1526-2359 (Electronic)
IS  - 1073-2748 (Print)
IS  - 1073-2748 (Linking)
VI  - 28
DP  - 2021 Jan-Dec
TI  - Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma.
PG  - 10732748211055681
LID - 10.1177/10732748211055681 [doi]
LID - 10732748211055681
AB  - BACKGROUND: As a new class of non-coding RNAs, circRNAs have been recently 
      reported to be involved in the tumorigenesis and progression of human cancers. In 
      the current study, we attempted to explore the potential function of a novel 
      circRNA (hsa_circ_0013290) in hepatocellular carcinoma (HCC). METHODS: Relative 
      hsa_circ_0013290 expression was analyzed by quantitative reverse 
      transcription-polymerase chain reaction (qRT-PCR). The subcellular location of 
      hsa_circ_0013290 was performed by RNA subcellular isolation and fluorescence in 
      situ hybridization (FISH) assays. The effect of hsa_circ_0013290 on proliferation 
      was detected by Cell Counting Kit-8 (CCK-8) assays. The effect of 
      hsa_circ_0013290 on cell cycle distribution and apoptosis was detected by flow 
      cytometry. The invasion and migration abilities of hsa_circ_0013290 were detected 
      by transwell assays. RESULTS: Hsa_circ_0013290 is significantly upregulated in 
      HCC cell lines and mainly located in cytoplasm of HCC cells. Hsa_circ_0013290 
      overexpression promotes cell invasion and migration and inhibits cell apoptosis. 
      In contrast, hsa_circ_0013290 knockdown impedes cell invasion and migration and 
      accelerates cell apoptosis. However, hsa_circ_0013290 did not affect cell 
      proliferation. CONCLUSIONS: Hsa_circ_0013290 is overexpressed in HCC cell lines 
      and is mainly located in the cytoplasm of HCC cells. Hsa_circ_0013290 promotes 
      cell invasion and migration, and inhibits cell apoptosis.
FAU - Luo, Xi
AU  - Luo X
AUID- ORCID: 0000-0002-7288-6870
AD  - Department of Clinical Laboratory, The Third People's Hospital of Nantong, 
      Nantong, China.
FAU - Liu, Yicun
AU  - Liu Y
AD  - Medical School of Nantong University, 117814Nantong University, Nantong, China.
FAU - Li, Han
AU  - Li H
AD  - Medical School of Nantong University, 117814Nantong University, Nantong, China.
FAU - Cheng, Tiaochun
AU  - Cheng T
AD  - Medical School of Nantong University, 117814Nantong University, Nantong, China.
FAU - Wu, Jianjun
AU  - Wu J
AD  - Department of Hepatobiliary Surgery, The Third People's Hospital of Nantong, 
      Nantong, China.
FAU - Chen, Lin
AU  - Chen L
AD  - Nantong Institute of Liver Disease, The Third People's Hospital of Nantong, 
      Nantong, China.
FAU - Ju, Linling
AU  - Ju L
AD  - Nantong Institute of Liver Disease, The Third People's Hospital of Nantong, 
      Nantong, China.
FAU - Cai, Weihua
AU  - Cai W
AD  - Department of Hepatobiliary Surgery, The Third People's Hospital of Nantong, 
      Nantong, China.
FAU - Bian, Zhaolian
AU  - Bian Z
AUID- ORCID: 0000-0002-0802-6497
AD  - Department of Gastroenterology, The Third People's Hospital of Nantong, Nantong, 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Control
JT  - Cancer control : journal of the Moffitt Cancer Center
JID - 9438457
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Apoptosis/genetics
MH  - Carcinogenesis/*genetics
MH  - Carcinoma, Hepatocellular/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Neoplasms/*genetics
MH  - RNA, Circular/*metabolism
MH  - Up-Regulation/genetics
PMC - PMC8606922
OTO - NOTNLM
OT  - apoptosis
OT  - cell proliferation
OT  - hepatocellular carcinoma
OT  - hsa_circ_0013290
OT  - metastasis
COIS- Declaration of conflicting interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2021/11/21 06:00
MHDA- 2022/02/05 06:00
PMCR- 2021/11/19
CRDT- 2021/11/20 05:19
PHST- 2021/11/20 05:19 [entrez]
PHST- 2021/11/21 06:00 [pubmed]
PHST- 2022/02/05 06:00 [medline]
PHST- 2021/11/19 00:00 [pmc-release]
AID - 10.1177_10732748211055681 [pii]
AID - 10.1177/10732748211055681 [doi]
PST - ppublish
SO  - Cancer Control. 2021 Jan-Dec;28:10732748211055681. doi: 
      10.1177/10732748211055681.