PMID- 34800143
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20220429
IS  - 1863-2661 (Electronic)
IS  - 1863-2653 (Print)
IS  - 1863-2653 (Linking)
VI  - 227
IP  - 3
DP  - 2022 Apr
TI  - Does a third intermediate model for the vomeronasal processing of information 
      exist? Insights from the macropodid neuroanatomy.
PG  - 881-899
LID - 10.1007/s00429-021-02425-2 [doi]
AB  - The study of the alpha-subunit of Gi2 and Go proteins in the accessory olfactory bulb 
      (AOB) was crucial for the identification of the two main families of vomeronasal 
      receptors, V1R and V2R. Both families are expressed in the rodent and lagomorph 
      AOBs, according to a segregated model characterized by topographical 
      anteroposterior zonation. Many mammal species have suffered from the 
      deterioration of the Galphao pathway and are categorized as belonging to the uniform 
      model. This scenario has been complicated by characterization of the AOB in the 
      tammar wallaby, Notamacropus eugenii, which appears to follow a third model of 
      vomeronasal organization featuring exclusive Galphao protein expression, referred to 
      as the intermediate model, which has not yet been replicated in any other 
      species. Our morphofunctional study of the vomeronasal system (VNS) in Bennett's 
      wallaby, Notamacropus rufogriseus, provides further information regarding this 
      third model of vomeronasal transduction. A comprehensive histological, lectin, 
      and immunohistochemical study of the Bennett's wallaby VNS was performed. 
      Anti-Galphao and anti-Galphai2 antibodies were particularly useful because they labeled 
      the transduction cascade of V2R and V1R receptors, respectively. Both G proteins 
      showed canonical immunohistochemical labeling in the vomeronasal organ and the 
      AOB, consistent with the anterior-posterior zonation of the segregated model. The 
      lectin Ulex europaeus agglutinin selectively labeled the anterior AOB, providing 
      additional evidence for the segregation of vomeronasal information in the 
      wallaby. Overall, the VNS of the Bennett's wallaby shows a degree of 
      differentiation and histochemical and neurochemical diversity comparable to 
      species with greater VNS development. The existence of the third intermediate 
      type in vomeronasal information processing reported in Notamacropus eugenii is 
      not supported by our lectin-histochemical and immunohistochemical findings in 
      Notamacropus rufogriseus.
CI  - (c) 2021. The Author(s).
FAU - Torres, Mateo V
AU  - Torres MV
AUID- ORCID: 0000-0002-7671-3379
AD  - Department of Anatomy, Animal Production and Clinical Veterinary Sciences, 
      Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero 
      s/n, 27002, Lugo, Spain.
FAU - Ortiz-Leal, Irene
AU  - Ortiz-Leal I
AUID- ORCID: 0000-0002-8468-3659
AD  - Department of Anatomy, Animal Production and Clinical Veterinary Sciences, 
      Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero 
      s/n, 27002, Lugo, Spain.
FAU - Villamayor, Paula R
AU  - Villamayor PR
AUID- ORCID: 0000-0002-7361-7795
AD  - Department of Anatomy, Animal Production and Clinical Veterinary Sciences, 
      Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero 
      s/n, 27002, Lugo, Spain.
AD  - Department of Zoology Genetics and Physical Anthropology, Faculty of Veterinary, 
      University of Santiago de Compostela, Lugo, Spain.
FAU - Ferreiro, Andrea
AU  - Ferreiro A
AD  - Marcelle Nature Park, Outeiro de Rei, Lugo, Spain.
FAU - Rois, Jose Luis
AU  - Rois JL
AD  - Marcelle Nature Park, Outeiro de Rei, Lugo, Spain.
FAU - Sanchez-Quinteiro, Pablo
AU  - Sanchez-Quinteiro P
AUID- ORCID: 0000-0002-9891-4817
AD  - Department of Anatomy, Animal Production and Clinical Veterinary Sciences, 
      Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero 
      s/n, 27002, Lugo, Spain. pablo.sanchez@usc.es.
LA  - eng
PT  - Journal Article
DEP - 20211120
PL  - Germany
TA  - Brain Struct Funct
JT  - Brain structure & function
JID - 101282001
SB  - IM
MH  - Animals
MH  - Mammals
MH  - *Neuroanatomy
MH  - Olfactory Bulb/metabolism
MH  - Rodentia
MH  - *Vomeronasal Organ
PMC - PMC8930919
OTO - NOTNLM
OT  - Accessory olfactory bulb
OT  - G proteins
OT  - Immunohistochemistry
OT  - Marsupials
OT  - Vomeronasal
OT  - Wallaby
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/11/21 06:00
MHDA- 2022/03/22 06:00
PMCR- 2021/11/20
CRDT- 2021/11/20 12:05
PHST- 2021/09/01 00:00 [received]
PHST- 2021/11/07 00:00 [accepted]
PHST- 2021/11/21 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2021/11/20 12:05 [entrez]
PHST- 2021/11/20 00:00 [pmc-release]
AID - 10.1007/s00429-021-02425-2 [pii]
AID - 2425 [pii]
AID - 10.1007/s00429-021-02425-2 [doi]
PST - ppublish
SO  - Brain Struct Funct. 2022 Apr;227(3):881-899. doi: 10.1007/s00429-021-02425-2. 
      Epub 2021 Nov 20.