PMID- 34800856 OWN - NLM STAT- MEDLINE DCOM- 20220302 LR - 20220302 IS - 1878-3252 (Electronic) IS - 0946-672X (Linking) VI - 69 DP - 2022 Jan TI - Selenium enhances the expression of miR-9, miR-124 and miR-29a during neural differentiation of bone marrow mesenchymal stem cells. PG - 126898 LID - S0946-672X(21)00188-7 [pii] LID - 10.1016/j.jtemb.2021.126898 [doi] AB - BACKGROUND: Selenium (Se) is a trace element that plays important role in antioxidant defense in the brain. Sodium selenite (Na(2)SeO(3)) is an inorganic salt of Se which has an antioxidant function. In the present study, we investigated the effect of Sodium selenite on the expression of important neuronal microRNAs during neural differentiation of bone marrow-derived stem cells (BMSCs). METHODS: Mesenchymal stem cells were collected from rat bone marrow and cultured in the Dulbecco's Modified Eagle Medium (DMEM) medium. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was conducted to determine the toxicity of Na(2)SeO(3). For neural induction, BMSCs were divided into control, Na(2)SeO(3) containing (10 ng/mL) and Na2SeO(3) free groups and cultured in DMEM medium supplemented with Isobutyl-l-methylxanthine (IBMX), Fibroblast growth factor 2 (FGF2), B27, Retinoic acid, and brain derived neurotrophic factor (BDNF) for 14 days. At the end of the differentiation, immunostaining against Microtubule associated protein 2 (Map-2) and Choline acetyltransferase (ChAT) proteins was performed. Also, the total RNA is extracted from control and neural differentiated cells using a special kit, and the expression of miR-9, miR-124, and miR-29a was analyzed using real-time polymerase chain reaction (RT-PCR). RESULTS: Increasing Na(2)SeO(3) concentrations had increasing toxicity; therefore, the concentration of 10 ng/mL was used as a supplement during neural differentiation. Examination of the expression of Map-2 and ChAT proteins showed that Na(2)SeO(3) increased the expression of them and consequently the neuronal differentiation of BMSCs. Na(2)SeO(3) also significantly increased the expression of miR-9, miR-124, and miR-29a in BMSCs undergoing neuronal differentiation. CONCLUSIONS: Our results suggest that the protective effect of selenium on neural differentiation of stem cells may be mediated through neuron specific microRNAs. This result further highlights the importance of selenium supplementation in preventing neuronal diseases. CI - Copyright (c) 2021 Elsevier GmbH. All rights reserved. FAU - Jalali, Hanieh AU - Jalali H AD - Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, No. 43, South Moffateh Ave., Tehran, 15719-14911, Iran. Electronic address: jalali@khu.ac.ir. FAU - Golchin, Hasti AU - Golchin H AD - Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, No. 43, South Moffateh Ave., Tehran, 15719-14911, Iran. Electronic address: hastigolchin78@gmail.com. FAU - Sadri, Zahra AU - Sadri Z AD - Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, No. 43, South Moffateh Ave., Tehran, 15719-14911, Iran. Electronic address: zahrasadri7076@gmail.com. FAU - Karimzadeh Bardei, Latifeh AU - Karimzadeh Bardei L AD - School of Biology, College of Science, University of Tehran, Engelab Ave., Tehran, 14155-6655, Iran. Electronic address: latifehkarimzadeh@gmail.com. FAU - Nabiuni, Mohammad AU - Nabiuni M AD - Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, No. 43, South Moffateh Ave., Tehran, 15719-14911, Iran. Electronic address: devbiokharazmi@gmail.com. LA - eng PT - Journal Article DEP - 20211106 PL - Germany TA - J Trace Elem Med Biol JT - Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) JID - 9508274 RN - 0 (Antioxidants) RN - 0 (MIRN124 microRNA, rat) RN - 0 (MIRN9 microRNA, rat) RN - 0 (MicroRNAs) RN - H6241UJ22B (Selenium) RN - HIW548RQ3W (Sodium Selenite) SB - IM MH - Animals MH - Antioxidants MH - Bone Marrow Cells MH - Cell Differentiation MH - Cells, Cultured MH - *Mesenchymal Stem Cells MH - *MicroRNAs/genetics MH - Rats MH - *Selenium/pharmacology MH - Sodium Selenite/pharmacology OTO - NOTNLM OT - Bone marrow stem cell OT - MicroRNA OT - Neuron OT - Selenium EDAT- 2021/11/21 06:00 MHDA- 2022/03/03 06:00 CRDT- 2021/11/20 20:22 PHST- 2021/04/15 00:00 [received] PHST- 2021/06/22 00:00 [revised] PHST- 2021/11/02 00:00 [accepted] PHST- 2021/11/21 06:00 [pubmed] PHST- 2022/03/03 06:00 [medline] PHST- 2021/11/20 20:22 [entrez] AID - S0946-672X(21)00188-7 [pii] AID - 10.1016/j.jtemb.2021.126898 [doi] PST - ppublish SO - J Trace Elem Med Biol. 2022 Jan;69:126898. doi: 10.1016/j.jtemb.2021.126898. Epub 2021 Nov 6.