PMID- 34802194
OWN - NLM
STAT- MEDLINE
DCOM- 20220304
LR  - 20230202
IS  - 1864-0648 (Electronic)
IS  - 1864-063X (Print)
IS  - 1864-063X (Linking)
VI  - 15
IP  - 2
DP  - 2022 Feb
TI  - Inactivation of multidrug-resistant bacteria and bacterial spores and generation 
      of high-potency bacterial vaccines using ultrashort pulsed lasers.
PG  - e202100207
LID - 10.1002/jbio.202100207 [doi]
AB  - Multidrug-resistant organisms (MDROs) represent a continuing healthcare crisis 
      with no definitive solution to date. An alternative to antibiotics is the 
      development of therapies and vaccines using biocompatible physical methods such 
      as ultrashort pulsed (USP) lasers, which have previously been shown to inactivate 
      pathogens while minimizing collateral damage to human cells, blood proteins, and 
      vaccine antigens. Here we demonstrate that visible USP laser treatment results in 
      bactericidal effect (>/=3-log load reduction) against clinically significant MDROs, 
      including methicillin-resistant Staphylococcus aureus and extended spectrum 
      beta-lactamase-producing Escherichia coli. Bacillus cereus endospores, which are 
      highly resistant to conventional chemical and physical treatments, were also 
      shown to be effectively inactivated by USP laser treatment, resulting in 
      sporicidal (>/=3-log load reduction) activity. Furthermore, we demonstrate that 
      administration of USP laser-inactivated E. coli whole-cell vaccines at dosages as 
      low as 10(5)  cfu equivalents without adjuvant was able to protect 100% of mice 
      against subsequent lethal challenge. Our findings open the possibility for 
      application of USP lasers in disinfection of hospital environments, therapy of 
      drug-resistant bacterial infections in skin or bloodstream via pheresis 
      modalities, and in the production of potent bacterial vaccines.
CI  - (c) 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
FAU - Tsen, Shaw-Wei David
AU  - Tsen SD
AUID- ORCID: 0000-0002-4153-2674
AD  - Department of Radiology, Washington University School of Medicine, St Louis, 
      Missouri, USA.
FAU - Popovich, John
AU  - Popovich J
AD  - The Biodesign Institute Center for Immunotherapy, Vaccines and Virotherapy, 
      Arizona State University, Tempe, Arizona, USA.
FAU - Hodges, Megan
AU  - Hodges M
AD  - School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
FAU - Haydel, Shelley E
AU  - Haydel SE
AUID- ORCID: 0000-0001-5542-5469
AD  - The Biodesign Institute Center for Immunotherapy, Vaccines and Virotherapy, 
      Arizona State University, Tempe, Arizona, USA.
AD  - School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
FAU - Tsen, Kong-Thon
AU  - Tsen KT
AD  - Department of Physics, Arizona State University, Tempe, Arizona, USA.
AD  - Center for Biophysics, Arizona State University, Tempe, Arizona, USA.
FAU - Sudlow, Gail
AU  - Sudlow G
AD  - Department of Radiology, Washington University School of Medicine, St Louis, 
      Missouri, USA.
FAU - Mueller, Elizabeth A
AU  - Mueller EA
AD  - Department of Biology, Washington University in St Louis, St Louis, Missouri, 
      USA.
FAU - Levin, Petra Anne
AU  - Levin PA
AD  - Department of Biology, Washington University in St Louis, St Louis, Missouri, 
      USA.
FAU - Achilefu, Samuel
AU  - Achilefu S
AD  - Department of Radiology, Washington University School of Medicine, St Louis, 
      Missouri, USA.
AD  - Department of Biochemistry and Molecular Biophysics, Washington University in St 
      Louis, St Louis, Missouri, USA.
AD  - Department of Biomedical Engineering, Washington University in St Louis, St 
      Louis, Missouri, USA.
LA  - eng
GR  - R35 GM127331/GM/NIGMS NIH HHS/United States
GR  - 3R01EB021048-04S1/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20211208
PL  - Germany
TA  - J Biophotonics
JT  - Journal of biophotonics
JID - 101318567
RN  - 0 (Bacterial Vaccines)
SB  - IM
MH  - Animals
MH  - Bacterial Vaccines
MH  - *Drug Resistance, Multiple, Bacterial
MH  - Escherichia coli
MH  - Lasers
MH  - *Methicillin-Resistant Staphylococcus aureus
MH  - Mice
MH  - Spores, Bacterial
PMC - PMC8934174
MID - NIHMS1785479
OTO - NOTNLM
OT  - bacterial spores
OT  - multidrug-resistant organisms
OT  - pathogen inactivation
OT  - ultrashort pulsed laser
OT  - vaccines
COIS- CONFLICTS OF INTEREST SDT and KT hold patents on "System and method for 
      inactivating microorganisms with a femtosecond laser" (publication no. 
      US20080299636 A1).
EDAT- 2021/11/22 06:00
MHDA- 2022/03/05 06:00
PMCR- 2023/02/01
CRDT- 2021/11/21 21:06
PHST- 2021/09/20 00:00 [revised]
PHST- 2021/07/08 00:00 [received]
PHST- 2021/10/22 00:00 [accepted]
PHST- 2021/11/22 06:00 [pubmed]
PHST- 2022/03/05 06:00 [medline]
PHST- 2021/11/21 21:06 [entrez]
PHST- 2023/02/01 00:00 [pmc-release]
AID - 10.1002/jbio.202100207 [doi]
PST - ppublish
SO  - J Biophotonics. 2022 Feb;15(2):e202100207. doi: 10.1002/jbio.202100207. Epub 2021 
      Dec 8.