PMID- 34802511
OWN - NLM
STAT- MEDLINE
DCOM- 20211123
LR  - 20211123
IS  - 1095-9939 (Electronic)
IS  - 0048-3575 (Linking)
VI  - 179
DP  - 2021 Nov
TI  - 3-Bromopyruvate-induced glycolysis inhibition impacts larval growth and 
      development and carbohydrate homeostasis in fall webworm, Hyphantria cunea Drury.
PG  - 104961
LID - S0048-3575(21)00192-9 [pii]
LID - 10.1016/j.pestbp.2021.104961 [doi]
AB  - As a typical glycolytic inhibitor, 3-bromopyruvate (3-BrPA) has been extensively 
      studied in cancer therapy in recent decades. However, few studies focused on 
      3-BrPA in regulating the growth and development of insects, and the relationship 
      and regulatory mechanism between glycolysis and chitin biosynthesis remain 
      largely unknown. The Hyphantria cunea, named fall webworm, is a notorious 
      defoliator, which caused a huge economic loss to agriculture and forestry. Here, 
      we investigated the effects of 3-BrPA on the growth and development, glycolysis, 
      carbohydrate homeostasis, as well as chitin synthesis in H. cunea larvae. To 
      elucidate the action mechanism of 3-BrPA on H. cunea will provide a new insight 
      for the control of this pest. The results showed that 3-BrPA dramatically 
      restrained the growth and development of H. cunea larvae and resulted in larval 
      lethality. Meanwhile, we confirmed that 3-BrPA caused a significant decrease in 
      carbohydrate, adenosine triphosphate (ATP), pyruvic acid (PA), and triglyceride 
      (TG) levels by inhibiting glycolysis in H. cunea larvae. Further studies 
      indicated that 3-BrPA significantly affected the activities of hexokinase (HK), 
      phosphofructokinase (PFK), pyruvate kinase (PK), glucose 6-phosphate 
      dehydrogenase (G6PDH) and trehalase, as well as expressions of the genes related 
      to glycolysis, resulting in carbohydrate homeostasis disorder. Moreover, it was 
      found that 3-BrPA enhanced 20-hydroxyecdysone (20E) signaling by upregulating 
      HcCYP306A1 and HcCYP314A1, two critical genes in 20E synthesis pathway, and 
      accelerated chitin synthesis by upregulating transcriptional levels of genes in 
      the chitin synthesis pathway in H. cunea larvae. Taken together, our findings 
      provide a novel insight into the mechanism of glycolytic inhibitor in regulating 
      the growth and development of insects, and lay a foundation for the potential 
      application of glycolytic inhibitors in pest control as well.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Qiu, Qian
AU  - Qiu Q
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zou, Haifeng
AU  - Zou H
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zou, Hang
AU  - Zou H
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Jing, Tianzhong
AU  - Jing T
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Li, XingPeng
AU  - Li X
AD  - School of Forestry, Beihua University, Jilin 132013, PR China.
FAU - Yan, Gaige
AU  - Yan G
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Geng, Nannan
AU  - Geng N
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zhang, Bihan
AU  - Zhang B
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zhang, Zhidong
AU  - Zhang Z
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zhang, Shengyu
AU  - Zhang S
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Yao, Bin
AU  - Yao B
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zhang, Guocai
AU  - Zhang G
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China. 
      Electronic address: zhang640308@126.com.
FAU - Zou, Chuanshan
AU  - Zou C
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China. 
      Electronic address: zoucs1980@126.com.
LA  - eng
PT  - Journal Article
DEP - 20210830
PL  - United States
TA  - Pestic Biochem Physiol
JT  - Pesticide biochemistry and physiology
JID - 1301573
RN  - 0 (Carbohydrates)
RN  - 0 (Pyruvates)
RN  - 63JMV04GRK (bromopyruvate)
SB  - IM
MH  - Animals
MH  - *Carbohydrates
MH  - *Glycolysis
MH  - Homeostasis
MH  - Larva
MH  - Pyruvates
OTO - NOTNLM
OT  - 3-bromopyruvate
OT  - Chitin synthesis
OT  - Glycolysis inhibition
OT  - Growth and development arrest
OT  - Hyphantria cunea
EDAT- 2021/11/23 06:00
MHDA- 2021/11/24 06:00
CRDT- 2021/11/22 05:42
PHST- 2021/06/02 00:00 [received]
PHST- 2021/08/26 00:00 [revised]
PHST- 2021/08/27 00:00 [accepted]
PHST- 2021/11/22 05:42 [entrez]
PHST- 2021/11/23 06:00 [pubmed]
PHST- 2021/11/24 06:00 [medline]
AID - S0048-3575(21)00192-9 [pii]
AID - 10.1016/j.pestbp.2021.104961 [doi]
PST - ppublish
SO  - Pestic Biochem Physiol. 2021 Nov;179:104961. doi: 10.1016/j.pestbp.2021.104961. 
      Epub 2021 Aug 30.