PMID- 34805687
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 6
IP  - 45
DP  - 2021 Nov 16
TI  - Recyclable Target Metal-Enhanced Fluorometric Naked Eye Aptasensor for the 
      Detection of Pb(2+) and Ag(+) Ions Based on the Structural Change of 
      CaSnO(3)@PDANS-Constrained GC-Rich ssDNA.
PG  - 30580-30597
LID - 10.1021/acsomega.1c04319 [doi]
AB  - Reliable, label-free, and ultraselective detection of Pb(2+) and Ag(+) ions is of 
      paramount importance for toxicology assessment, human health, and environmental 
      protection. Herein, we present a novel recyclable fluorometric aptasensor based 
      on the Pb(2+) and Ag(+)-induced structural change of the GC-rich ssDNA (guanine 
      cytosine-rich single-strand DNA) and the differences in the fluorescence emission 
      of acridine orange (AO) from random coil to highly stable G-quadruplex for the 
      detection of Pb(2+) and Ag(+) ions. More interestingly, the construction and 
      principle of the aptasensor explore that the GC-rich ssDNA and AO can be strongly 
      adsorbed on the CaSnO(3)@PDANS surface through the pi-pi stacking, 
      hydrogen-bonding, and metal coordination interactions, which exhibit high 
      fluorescence quenching and robust holding of the GC-rich ssDNA. However, in the 
      presence of Pb(2+), the specific G-rich ssDNA segment could form a stable 
      G-quadruplex via G4-Pb(2+) coordination and capture of AO from the CaSnO(3)@PDANS 
      surface resulting in fluorescence recovery (70% enhancement). The subsequent 
      addition of Ag(+) ion induces coupled cytosine base pairs in another segment of 
      ssDNA to get folded into a duplex structure together with the G-quadruplex, which 
      highly stabilizes the G-quadruplex resulting in the maximum recovery of AO 
      emission (99% enhancement). When the Cys@Fe(3)O(4)Nps are added to the above 
      solution, the sensing probe was restored by complexation between the Cys in the 
      Cys@Fe(3)O(4)Nps and target metal ions, resulting in the fabrication of a highly 
      sensitive recyclable Pb(2+) and Ag(+) assay with detection limits of 0.4 and 0.1 
      nM, respectively. Remarkably, the Cys@Fe(3)O(4)Nps can also be reused after 
      washing with EDTA. The utility of the proposed approach has great potential for 
      detecting the Pb(2+) and Ag(+) ions in environmental samples with interfering 
      contaminants.
CI  - (c) 2021 The Authors. Published by American Chemical Society.
FAU - Amalraj, Arunjegan
AU  - Amalraj A
AD  - Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur 
      603203, Tamil Nadu, India.
FAU - Pavadai, Rajaji
AU  - Pavadai R
AD  - Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur 
      603203, Tamil Nadu, India.
FAU - Perumal, Panneerselvam
AU  - Perumal P
AUID- ORCID: 0000-0003-2647-6835
AD  - Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur 
      603203, Tamil Nadu, India.
LA  - eng
PT  - Journal Article
DEP - 20211103
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC8600652
COIS- The authors declare no competing financial interest.
EDAT- 2021/11/23 06:00
MHDA- 2021/11/23 06:01
PMCR- 2021/11/03
CRDT- 2021/11/22 06:58
PHST- 2021/08/11 00:00 [received]
PHST- 2021/09/29 00:00 [accepted]
PHST- 2021/11/22 06:58 [entrez]
PHST- 2021/11/23 06:00 [pubmed]
PHST- 2021/11/23 06:01 [medline]
PHST- 2021/11/03 00:00 [pmc-release]
AID - 10.1021/acsomega.1c04319 [doi]
PST - epublish
SO  - ACS Omega. 2021 Nov 3;6(45):30580-30597. doi: 10.1021/acsomega.1c04319. 
      eCollection 2021 Nov 16.