PMID- 34808452
OWN - NLM
STAT- MEDLINE
DCOM- 20220218
LR  - 20220218
IS  - 2300-732X (Electronic)
IS  - 1642-431X (Linking)
VI  - 21
IP  - 4
DP  - 2021 Dec
TI  - Upregulated pigment epithelium-derived factor (PEDF) promotes trophoblast 
      apoptosis and inhibits invasion in preeclampsia.
PG  - 100576
LID - S1642-431X(21)00097-8 [pii]
LID - 10.1016/j.repbio.2021.100576 [doi]
AB  - Preeclampsia (PE) is a severe pregnancy-specific disorder. Previous findings 
      indicated that pigment epithelium-derived factor (PEDF) was upregulated in 
      placentas of women with PE. Here, we investigated the role of PEDF in trophoblast 
      function, especially under hypoxia. The effects of hypoxia on the morphology of 
      extravillous trophoblast (EVT)-derived HTR-8Svneo cells were observed under 
      inverted microscope. Transfections with Lipofectamine LTX were performed 
      according to the manufacturer's protocol. The expression of PEDF protein and mRNA 
      were confirmed by immunofluorescence (IF) and quantitative real-time PCR (qPCR). 
      Apoptosis was detected by transferase-mediated dUTP nick end labeling (TUNEL) 
      assay, and proliferation of trophoblast was detected by CCK-8 method. The 
      invasion capacity of trophoblast was assessed by Transwell assay. PEDF was 
      expressed in HTR-8/SVneo under both normoxia and hypoxic stress. However, cells 
      of hypoxia groups had higher expression level of PEDF, increased apoptosis and 
      decreased invasion capability, as compared with normoxia group. Moreover, after 
      transfection with plasmid expressing PEDF gene, overexpression of PEDF modulated 
      trophoblast activities. In addition, PEDF expression was negatively associated 
      with invasion while positively correlated with apoptosis.Our data suggest that 
      PEDF is an important factor to maintain the biological function of trophoblast 
      cells, thus representing a rational therapeutic target in PE.
CI  - Copyright (c) 2021 Society for Biology of Reproduction & the Institute of Animal 
      Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. 
      Published by Elsevier B.V. All rights reserved.
FAU - Wu, Ying
AU  - Wu Y
AD  - Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital 
      Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, 
      China.
FAU - Chen, Xuexin
AU  - Chen X
AD  - Department of Biochemistry and Molecular Biology, GMU-GIBH Joint School of Life 
      Sciences, Guangzhou Medical University, Guangzhou, China.
FAU - Jiao, Simeng
AU  - Jiao S
AD  - Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital 
      Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, 
      China.
FAU - Wang, Yiqian
AU  - Wang Y
AD  - Department of Biochemistry and Molecular Biology, GMU-GIBH Joint School of Life 
      Sciences, Guangzhou Medical University, Guangzhou, China. Electronic address: 
      you52329@163.com.
LA  - eng
PT  - Journal Article
DEP - 20211119
PL  - Poland
TA  - Reprod Biol
JT  - Reproductive biology
JID - 101160559
RN  - 0 (Eye Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Serpins)
RN  - 0 (pigment epithelium-derived factor)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Apoptosis/*drug effects/physiology
MH  - Cell Line
MH  - Cell Movement/physiology
MH  - Eye Proteins/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Oxygen/*pharmacology
MH  - Pre-Eclampsia/*metabolism
MH  - Pregnancy
MH  - Serpins/genetics/*metabolism
MH  - Trophoblasts/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Hypoxia
OT  - Invasion
OT  - PEDF
OT  - Proliferation
OT  - Trophoblast
COIS- Declaration of Competing Interest The authors do not have conflicts of interest 
      to declare.
EDAT- 2021/11/23 06:00
MHDA- 2022/02/19 06:00
CRDT- 2021/11/22 20:20
PHST- 2021/09/17 00:00 [received]
PHST- 2021/11/03 00:00 [revised]
PHST- 2021/11/10 00:00 [accepted]
PHST- 2021/11/23 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2021/11/22 20:20 [entrez]
AID - S1642-431X(21)00097-8 [pii]
AID - 10.1016/j.repbio.2021.100576 [doi]
PST - ppublish
SO  - Reprod Biol. 2021 Dec;21(4):100576. doi: 10.1016/j.repbio.2021.100576. Epub 2021 
      Nov 19.