PMID- 34812690
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220316
IS  - 1753-4267 (Electronic)
IS  - 1753-4259 (Print)
IS  - 1753-4259 (Linking)
VI  - 27
IP  - 7-8
DP  - 2021 Oct
TI  - Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and 
      suppressing NF-kappaB.
PG  - 543-552
LID - 10.1177/17534259211062553 [doi]
AB  - Acute lung injury (ALI) is associated with a high mortality due to inflammatory 
      cell infiltration and lung edema. The development of ALI commonly involves the 
      activation of NF-kappaB. Since bergamottin is a natural furanocoumarin showing the 
      ability to inhibit the activation of NF-kappaB, in this study we aimed to determine 
      the effect of bergamottin on ALI. RAW264.7 mouse macrophages were pre-treated 
      with bergamottin and then stimulated with LPS. Macrophage inflammatory responses 
      were examined. Bergamottin (50 mg/kg body mass) was intraperitoneally 
      administrated to mice 12 h before injection of LPS, and the effect of bergamottin 
      on LPS-induced ALI was evaluated. Our results showed that LPS exposure led to 
      increased production of TNF-alpha, IL-6, and monocyte chemoattractant protein-1 
      (MCP-1), which was impaired by bergamottin pre-treatment. In vivo studies 
      confirmed that bergamottin pre-treatment suppressed LPS-induced lung inflammation 
      and edema and reduced the levels of pro-inflammatory cytokines in lung tissues 
      and bronchoalveolar lavage fluids. Mechanistically, bergamottin blocked 
      LPS-induced activation of NF-kappaB signaling in lung tissues. Additionally, 
      bergamottin treatment reduced NF-kappaB p65 protein acetylation, which was coupled 
      with induction of SIRT1 expression. In conclusion, our results reveal the 
      anti-inflammatory property of bergamottin in preventing ALI. Induction of SIRT1 
      and inhibition of NF-kappaB underlies the anti-inflammatory activity of bergamottin.
FAU - An, Ning
AU  - An N
AD  - Institue of Anesthesia and Critical Care Medicine, Union Hospital, Tongji Medical 
      College, 12443Huazhong University of Science and Technology, Wuhan, China.
FAU - Yang, Tao
AU  - Yang T
AD  - Department of Intensive Care Unit, Wuhan Fourth Hospital, Puai Hospital, Tongji 
      Medical College, 12443Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Zhang, Xiao-Xia
AU  - Zhang XX
AD  - Department of Intensive Care Unit, Wuhan Fourth Hospital, Puai Hospital, Tongji 
      Medical College, 12443Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Xu, Mei-Xia
AU  - Xu MX
AUID- ORCID: 0000-0003-2667-1577
AD  - Department of Intensive Care Unit, Wuhan Fourth Hospital, Puai Hospital, Tongji 
      Medical College, 12443Huazhong University of Science and Technology, Wuhan, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211123
PL  - United States
TA  - Innate Immun
JT  - Innate immunity
JID - 101469670
RN  - 0 (Cytokines)
RN  - 0 (Furocoumarins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - EC 3.5.1.- (Sirt1 protein, mouse)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - JMU611YFRB (bergamottin)
SB  - IM
MH  - *Acute Lung Injury/chemically induced/drug therapy/metabolism
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid
MH  - Cytokines/metabolism
MH  - *Furocoumarins/adverse effects
MH  - Lipopolysaccharides/pharmacology
MH  - Lung
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Sirtuin 1
PMC - PMC8762093
OTO - NOTNLM
OT  - Acetylation
OT  - NF-kappaB
OT  - SIRT1
OT  - bergamottin
OT  - lung inflammation
COIS- Declaration of conflicting interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2021/11/24 06:00
MHDA- 2022/03/17 06:00
PMCR- 2021/11/23
CRDT- 2021/11/23 12:15
PHST- 2021/11/24 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
PHST- 2021/11/23 12:15 [entrez]
PHST- 2021/11/23 00:00 [pmc-release]
AID - 10.1177_17534259211062553 [pii]
AID - 10.1177/17534259211062553 [doi]
PST - ppublish
SO  - Innate Immun. 2021 Oct;27(7-8):543-552. doi: 10.1177/17534259211062553. Epub 2021 
      Nov 23.