PMID- 34813127
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220401
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 85
IP  - 4
DP  - 2022 Apr
TI  - Physiological and biochemical responses of Brassica napus L. to drought-induced 
      stress by the application of biochar and Plant Growth Promoting Rhizobacteria.
PG  - 1267-1281
LID - 10.1002/jemt.23993 [doi]
AB  - Climate change induces biotic and abiotic stress conditions, which badly affect 
      the yield of crops with leading to the biochemical and physiological damages to 
      plants. Biochar and plant growth promoting rhizobacteria (PGPR) alleviate the 
      effect of drought condition therefore a field study was conducted to examine the 
      single and combine application of drought tolerant Pseudomonas sp. and 
      Staphylococcus sp. with biochar of Morus alba L. wood to mitigate the adverse 
      effects of drought stress in two genotypes of Brassica napus L. including Punjab 
      sarson and westar. Physioco-chemical analysis of biochar showed 5.4 cmol/kg 
      cation exchange capacity, 6.9 ds/m electrical conductivity, pH of 9.6, 
      0.50 g/cm(3) bulk density, and organic carbon 3.64%. Synergistic application of 
      PGPR and biochar developed the plant antioxidant enzyme including catalase (CAT) 
      and ascorbate peroxidase (APX) and also enhanced the content of photosynthetic 
      pigments like chlorophyll pigments, carotenoids content, and anthocyanin content. 
      Scanning electron microscope (SEM) study revealed that biochar and PGPR improved 
      epidermal vigor and stomatal physiology. Malondialdehyde (MDA), hydrogen peroxide 
      (H(2) O(2) ), APX, and osmolyte content including proline increased in drought 
      stress, which were then decreased by these growth promoters. These results are 
      very important as they illustrate the potential of PGPR and biochar to alleviate 
      the adverse consequences of drought stress and offer a way of increasing the 
      tolerance of B. napus L. plant grown under induced drought stress.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Lalay, Gul
AU  - Lalay G
AUID- ORCID: 0000-0002-2054-7981
AD  - Department of Botany, University of Peshawar, Peshawar, Pakistan.
FAU - Ullah, Sami
AU  - Ullah S
AD  - Department of Botany, University of Peshawar, Peshawar, Pakistan.
FAU - Ahmed, Iftikhar
AU  - Ahmed I
AUID- ORCID: 0000-0003-0969-7927
AD  - National Culture Collection of Pakistan, Bio-resources Conservation Institute 
      (BCI), National Agriculture Research Center, Islamabad, Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20211123
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (biochar)
RN  - 1406-65-1 (Chlorophyll)
RN  - 16291-96-6 (Charcoal)
SB  - IM
MH  - *Brassica napus
MH  - Charcoal
MH  - Chlorophyll/pharmacology
MH  - *Droughts
MH  - Stress, Physiological
OTO - NOTNLM
OT  - Pseudomonas sp.
OT  - Staphylococcus sp.
OT  - antioxidant enzyme
OT  - climate change
OT  - osmolyte content
EDAT- 2021/11/24 06:00
MHDA- 2022/04/01 06:00
CRDT- 2021/11/23 12:31
PHST- 2021/10/21 00:00 [revised]
PHST- 2021/07/29 00:00 [received]
PHST- 2021/11/05 00:00 [accepted]
PHST- 2021/11/24 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2021/11/23 12:31 [entrez]
AID - 10.1002/jemt.23993 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2022 Apr;85(4):1267-1281. doi: 10.1002/jemt.23993. Epub 2021 
      Nov 23.