PMID- 34813790
OWN - NLM
STAT- MEDLINE
DCOM- 20220103
LR  - 20220103
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 194
DP  - 2022 Jan 1
TI  - Isolation and immune activity of a new acidic Cordyceps militaris 
      exopolysaccharide.
PG  - 706-714
LID - S0141-8130(21)02520-4 [pii]
LID - 10.1016/j.ijbiomac.2021.11.115 [doi]
AB  - A new type of acidic exopolysaccharide (AESP-II) was extracted and separated from 
      the fermentation broth of Cordyceps militaris (C. militaris), which was further 
      purified to elucidate its structural characteristics and immunological activity. 
      AESP-II was confirmed to be an acidic pyranose with a molecular weight of 
      61.52 kDa, which consisted of mannose, glucuronic acid, rhamnose, galactose acid, 
      N-acetyl-galactosamine, glucose, galactose and arabinose with a molar ratio of 
      1.07: 5.38: 1: 3.14: 2.23: 15: 6.09: and 4.04. Animal experiment results verified 
      that AESP-II can significantly promote the proliferation of spleen T and B 
      lymphocytes in mice with immune injury caused by cyclophosphamide (CTX). In 
      particular, the promotion of B lymphocytes presented a dose-effect relationship. 
      In addition, the levels of the cytokines IL-2, IL-4, and IFN-gamma, which are mainly 
      secreted by T lymphocytes, and immunoglobulin IgG, IgM and IgA, which are mainly 
      secreted by B lymphocytes, were increased after AESP-II treatment. The above 
      results suggest that fluid immunity is involved in the immunomodulatory function 
      of AESP-II. Simultaneously, AESP-II was detected significantly to promote the 
      phosphorylation expression of p38 kinase (p38), extracellular regulated protein 
      kinases (ERK) and c-Jun N-terminal kinase (JNK) by Western blot, further 
      suggesting that the activation of the MAPK signaling pathway mediates the 
      immunoregulatory function of AESP-II.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Yu, Yue
AU  - Yu Y
AD  - Graduate School of Food Science and Engineering, Jilin Agricultural University, 
      Changchun 130118, PR China.
FAU - Wen, Qiang
AU  - Wen Q
AD  - National Institutes for Food and Drug Control, Beijing 102629, PR China.
FAU - Song, Ao
AU  - Song A
AD  - Changchun Customs Technology Center, Changchun 130033, PR China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Changchun Customs Technology Center, Changchun 130033, PR China.
FAU - Wang, Fei
AU  - Wang F
AD  - School of Life Science, Liaocheng University, Liaocheng 252059, PR China. 
      Electronic address: wangfei0801@126.com.
FAU - Jiang, Bin
AU  - Jiang B
AD  - Graduate School of Food Science and Engineering, Jilin Agricultural University, 
      Changchun 130118, PR China. Electronic address: binj@jlau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20211120
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Cordyceps/immunology/metabolism
MH  - Female
MH  - Lymphocytes/cytology/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Polysaccharides/immunology/isolation & purification
OTO - NOTNLM
OT  - Acidic exopolysaccharide
OT  - C. militaris strain
OT  - Fermentation broth
OT  - Immune activity
OT  - Polysaccharide structure
EDAT- 2021/11/24 06:00
MHDA- 2022/01/04 06:00
CRDT- 2021/11/23 20:12
PHST- 2021/01/22 00:00 [received]
PHST- 2021/10/21 00:00 [revised]
PHST- 2021/11/16 00:00 [accepted]
PHST- 2021/11/24 06:00 [pubmed]
PHST- 2022/01/04 06:00 [medline]
PHST- 2021/11/23 20:12 [entrez]
AID - S0141-8130(21)02520-4 [pii]
AID - 10.1016/j.ijbiomac.2021.11.115 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2022 Jan 1;194:706-714. doi: 10.1016/j.ijbiomac.2021.11.115. 
      Epub 2021 Nov 20.