PMID- 34817805
OWN - NLM
STAT- MEDLINE
DCOM- 20220202
LR  - 20220202
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 194
IP  - 1
DP  - 2022 Jan
TI  - Designing of a Chimeric Vaccine Using EIS (Rv2416c) Protein Against Mycobacterium 
      tuberculosis H37Rv: an Immunoinformatics Approach.
PG  - 187-214
LID - 10.1007/s12010-021-03760-0 [doi]
AB  - Mycobacterium tuberculosis (Mtb) is a respiratory pathogen that causes 
      tuberculosis (TB). There are a large number of proteins that are involved in the 
      pathogenesis of TB. Stimulating the immune response against TB is very important 
      to clear the pathogens from host. In the present study, an immunoinformatics 
      conduit is used for designing an epitope based chimeric vaccine against TB. 
      Enhanced intracellular survival (EIS) protein from Mtb is used for designing the 
      chimeric vaccine. One B cell epitope, 8 cytotoxic T lymphocyte (CTL), and 6 
      helper T lymphocyte (HTL) epitopes were predicted based on the MHC allele 
      binding, immunogenicity, antigenicity, allergenicity, toxicity and IFN epitopes. 
      The selected epitopes were used for chimeric vaccine designing. Furthermore, 3D 
      structure elucidation, structural refinement and validation of the designed 
      chimeric vaccine were carried out. The 3D structure was used for protein-protein 
      docking studies with Toll-like receptor 4 (TLR-4), followed by molecular dynamic 
      simulation (MDS) and the interaction between the chimeric vaccine and TLR-4 
      complex was verified.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Logesh, R
AU  - Logesh R
AD  - School of Life Sciences, B. S. Abdur Rahman Crescent Institute of Science & 
      Technology, Vandalur, Chennai, 600048, Tamil Nadu, India.
FAU - Lavanya, V
AU  - Lavanya V
AD  - Department of Biotechnology, Guru Nanak College, Velachery, Chennai, 600042, 
      Tamil Nadu, India.
FAU - Jamal, Shazia
AU  - Jamal S
AD  - School of Life Sciences, B. S. Abdur Rahman Crescent Institute of Science & 
      Technology, Vandalur, Chennai, 600048, Tamil Nadu, India.
FAU - Ahmed, Neesar
AU  - Ahmed N
AUID- ORCID: 0000-0002-7367-2188
AD  - School of Life Sciences, B. S. Abdur Rahman Crescent Institute of Science & 
      Technology, Vandalur, Chennai, 600048, Tamil Nadu, India. 
      neesar.sls@crescent.education.
LA  - eng
PT  - Journal Article
DEP - 20211124
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Tuberculosis Vaccines)
RN  - 0 (Vaccines, Subunit)
SB  - IM
MH  - *Epitopes, B-Lymphocyte/genetics/immunology
MH  - *Epitopes, T-Lymphocyte/genetics/immunology
MH  - Humans
MH  - *Molecular Docking Simulation
MH  - *Molecular Dynamics Simulation
MH  - *Mycobacterium tuberculosis/genetics/immunology
MH  - *Tuberculosis Vaccines/genetics/immunology
MH  - Vaccines, Subunit/genetics/immunology
OTO - NOTNLM
OT  - Chimeric vaccine
OT  - EIS
OT  - Immunoinformatics
OT  - TLR-4
OT  - Tuberculosis
EDAT- 2021/11/25 06:00
MHDA- 2022/02/03 06:00
CRDT- 2021/11/24 12:30
PHST- 2021/07/19 00:00 [received]
PHST- 2021/11/08 00:00 [accepted]
PHST- 2021/11/25 06:00 [pubmed]
PHST- 2022/02/03 06:00 [medline]
PHST- 2021/11/24 12:30 [entrez]
AID - 10.1007/s12010-021-03760-0 [pii]
AID - 10.1007/s12010-021-03760-0 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2022 Jan;194(1):187-214. doi: 
      10.1007/s12010-021-03760-0. Epub 2021 Nov 24.