PMID- 34822812
OWN - NLM
STAT- MEDLINE
DCOM- 20211227
LR  - 20211227
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 409
IP  - 2
DP  - 2021 Dec 15
TI  - Human umbilical cord mesenchymal stem cells regulate CD54 and CD105 in vascular 
      endothelial cells and suppress inflammation in Kawasaki disease.
PG  - 112941
LID - S0014-4827(21)00497-3 [pii]
LID - 10.1016/j.yexcr.2021.112941 [doi]
AB  - OBJECTIVE: The objective was to evaluate the expression levels of CD31(+)CD54(+) 
      and CD31(+)CD105(+) endothelial microparticles (EMPs) before and after 
      intravenous immunoglobulin (IVIG) treatment of Kawasaki disease (KD). To explore 
      the role of human umbilical cord mesenchymal stem cells (hucMSCs) in inhibiting 
      endothelial inflammation in KD, the effects of hucMSCs on the expression of CD54 
      and CD105 in endothelial cells in KD were analyzed in vivo and in vitro. METHODS: 
      The concentrations of IL-1beta and VEGF in the peripheral blood of KD or healthy 
      children were detected, and the distributions of CD31(+)CD54(+) and 
      CD31(+)CD105(+) EMPs in platelet-poor plasma (PPP) were analyzed by flow 
      cytometry. Human umbilical vein endothelial cells (HUVECs) were first cocultured 
      with the patients' peripheral blood mononuclear cells (PBMCs). Next, HUVECs were 
      cocultured with hucMSCs after stimulation with inactivated serum from patients. 
      Cell proliferation and migration activities were assessed, and the expression of 
      CD54, CD105 and IL-1beta was analyzed. In an in vivo study, hucMSCs were 
      transplanted into KD mice. The locations and expression levels of CD54, CD105 and 
      IL-1beta in the heart tissues of mice were analyzed. RESULTS: The levels of IL-1beta 
      and CD31(+)CD54(+) EMPs were significantly higher before IVIG treatment and 2 
      weeks after treatment in KD patients (P < 0.01). However, the levels of VEGF and 
      CD31(+)CD105(+) EMPs increased significantly in KD only after IVIG treatment 
      (P < 0.01). KD-inactivated serum stimulation combined with cocultivation of PBMCs 
      can activate inflammation in HUVECs, leading to reduced cell proliferation and 
      migration activities. Cocultivation also increased the expression of CD54 and 
      decreased the expression of CD105 (P < 0.001). Cocultivation with hucMSCs can 
      reverse these changes. Additionally, hucMSC transplantation downregulated the 
      expression of IL-1beta and CD54 and significantly upregulated the expression of 
      CD105 in KD mice. CONCLUSION: The expression levels of CD31(+)CD54(+) and 
      CD31(+)CD105(+) EMPs showed inconsistent changes at different KD statuses, 
      providing potential markers for clinical application. HucMSCs suppress 
      inflammation and regulate the expression levels of CD54 and CD105 in vascular 
      endothelial cells in KD, possibly providing a new basis for stem cell therapy for 
      KD.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Chen, Tao
AU  - Chen T
AD  - Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 
      226001, Jiangsu Province, China; Research Institute of Comparative Medicine, 
      Nantong University, Nantong, 226001, Jiangsu Province, China.
FAU - Xu, Ting
AU  - Xu T
AD  - Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 
      226001, Jiangsu Province, China; Research Institute of Comparative Medicine, 
      Nantong University, Nantong, 226001, Jiangsu Province, China.
FAU - Cheng, Mingye
AU  - Cheng M
AD  - Department of Pediatrics, Zhongda Hospital Southeast University, Nanjing, 210009, 
      Jiangsu Province, China.
FAU - Fang, Hao
AU  - Fang H
AD  - Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 
      226001, Jiangsu Province, China.
FAU - Shen, Xianjuan
AU  - Shen X
AD  - Department of Clinical Laboratory, Affiliated Hospital of Nantong University, 
      Nantong, 226001, Jiangsu Province, China.
FAU - Tang, Zhiyuan
AU  - Tang Z
AD  - Department of Pharmacy, Affiliated Hospital of Nantong University, Nantong, 
      226001, Jiangsu, China. Electronic address: tina2951@sina.com.
FAU - Zhao, Jianmei
AU  - Zhao J
AD  - Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 
      226001, Jiangsu Province, China. Electronic address: zjmheart@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211122
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Biomarkers)
RN  - 0 (Endoglin)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Case-Control Studies
MH  - Cell Differentiation
MH  - Child, Preschool
MH  - Disease Models, Animal
MH  - Endoglin/*metabolism
MH  - Female
MH  - Human Umbilical Vein Endothelial Cells/immunology/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mucocutaneous Lymph Node Syndrome/complications/metabolism/pathology/*therapy
MH  - Prognosis
MH  - Umbilical Cord/*cytology
MH  - Vasculitis/etiology/pathology/*prevention & control
OTO - NOTNLM
OT  - Endothelial microparticles
OT  - Human umbilical cord mesenchymal stem cells
OT  - Kawasaki disease
OT  - Stem cell therapy
EDAT- 2021/11/26 06:00
MHDA- 2021/12/28 06:00
CRDT- 2021/11/25 20:10
PHST- 2021/07/18 00:00 [received]
PHST- 2021/11/15 00:00 [revised]
PHST- 2021/11/18 00:00 [accepted]
PHST- 2021/11/26 06:00 [pubmed]
PHST- 2021/12/28 06:00 [medline]
PHST- 2021/11/25 20:10 [entrez]
AID - S0014-4827(21)00497-3 [pii]
AID - 10.1016/j.yexcr.2021.112941 [doi]
PST - ppublish
SO  - Exp Cell Res. 2021 Dec 15;409(2):112941. doi: 10.1016/j.yexcr.2021.112941. Epub 
      2021 Nov 22.