PMID- 34823185
OWN - NLM
STAT- MEDLINE
DCOM- 20211222
LR  - 20211222
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 101
IP  - 1
DP  - 2022 Jan
TI  - Fumonisin B(1) induces chicken heterophil extracellular traps mediated by PAD4 
      enzyme and P2 x 1 receptor.
PG  - 101550
LID - S0032-5791(21)00572-1 [pii]
LID - 10.1016/j.psj.2021.101550 [doi]
LID - 101550
AB  - Fumonisin B(1) (FB(1)) is a common mycotoxin contamination in agricultural 
      commodities being considered as a significant risk to human and livestock health, 
      while the mechanism of FB(1) immunotoxicity are less understood, especially in 
      chicken. Given that extracellular traps as a novel defense mechanism of 
      leukocytes play an important role against foreign matters, in this study we aimed 
      to investigate the effects of FB(1) on chicken heterophil extracellular traps 
      (HETs) formation. Our result showed that FB(1) induced HETs release in chicken 
      heterophils observed via immunostaining, and it was concentration-dependent 
      during 10 to 40 muM. Moreover, in 40 muM FB(1)-exposed chicken heterophils, 
      reactive oxygen species (ROS) level was increased, while catalase (CAT), 
      superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity and 
      glutathione (GSH) content were decreased. Simultaneously, FB(1) (40 muM) activated 
      ERK and p38 MAPK signaling pathways via increasing the phosphorylation level of 
      ERK and p38 proteins. However, pretreatment of SB202190, U0126, and 
      diphenyleneiodonium chloride (DPI) did not change FB(1)-triggered ROS production 
      and HETs formation, suggesting FB(1)-induced HETs was a nicotinamide adenine 
      dinucleotide phosphate (NADPH) oxidase, p38, and extracellular regulated protein 
      kinases (ERK) signaling pathways-independent process. Inhibition of peptidyl 
      arginine deiminase 4 (PAD4) enzyme and P2 x 1 receptor showed their vital role in 
      40 muM FB(1)-triggered HETs. This study reported for the first time that 40 muM 
      FB(1) induced the release of HETs in heterophils, and it was related to ROS 
      production, PAD4, and P2 x 1, but was independent of NADPH oxidase, p38 and ERK 
      signaling pathways, which might provide a whole novel perspective of perceiving 
      and understanding the role of FB(1) in immunotoxicity.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Wu, Zhikai
AU  - Wu Z
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Zhu, Xingyi
AU  - Zhu X
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Li, Peixuan
AU  - Li P
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Wang, Xia
AU  - Wang X
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Sun, Youpeng
AU  - Sun Y
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Fu, Yiwu
AU  - Fu Y
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Wang, Jingjing
AU  - Wang J
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Yang, Zhengtao
AU  - Yang Z
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China.
FAU - Zhou, Ershun
AU  - Zhou E
AD  - College of Life Sciences and Engineering, Foshan University, Foshan 528225, 
      Guangdong Province, PR China. Electronic address: zhouershun@fosu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20211020
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Fumonisins)
RN  - 3ZZM97XZ32 (fumonisin B1)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - *Extracellular Traps
MH  - Fumonisins
PMC - PMC8626696
OTO - NOTNLM
OT  - chicken
OT  - fumonisin B(1)
OT  - heterophil extracellular traps
OT  - immunotoxicity
EDAT- 2021/11/26 06:00
MHDA- 2021/12/24 06:00
PMCR- 2021/10/20
CRDT- 2021/11/25 20:22
PHST- 2021/08/30 00:00 [received]
PHST- 2021/10/10 00:00 [revised]
PHST- 2021/10/12 00:00 [accepted]
PHST- 2021/11/26 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2021/11/25 20:22 [entrez]
PHST- 2021/10/20 00:00 [pmc-release]
AID - S0032-5791(21)00572-1 [pii]
AID - 101550 [pii]
AID - 10.1016/j.psj.2021.101550 [doi]
PST - ppublish
SO  - Poult Sci. 2022 Jan;101(1):101550. doi: 10.1016/j.psj.2021.101550. Epub 2021 Oct 
      20.