PMID- 34826339
OWN - NLM
STAT- MEDLINE
DCOM- 20220118
LR  - 20220118
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 57
IP  - 1
DP  - 2022 Jan
TI  - Exosomes from dental pulp cells attenuate bone loss in mouse experimental 
      periodontitis.
PG  - 162-172
LID - 10.1111/jre.12949 [doi]
AB  - BACKGROUND AND OBJECTIVE: Exosomes are small vesicles secreted from many cell 
      types. Their biological effects largely depend on their cellular origin and the 
      physiological state of the originating cells. Exosomes secreted by mesenchymal 
      stem cells exert therapeutic effects against multiple diseases and may serve as 
      potential alternatives to stem cell therapies. We previously established and 
      characterized human leukocyte antigen (HLA) haplotype homo (HHH) dental pulp cell 
      (DPC) lines from human wisdom teeth. In this study, we aimed to investigate the 
      effect of local administration of HHH-DPC exosomes in a mouse model of 
      periodontitis. METHODS: Exosomes purified from HHH-DPCs were subjected to 
      particle size analysis, and expression of exosome markers was confirmed by 
      western blotting. We also confirmed the effect of exosomes on the migration of 
      both HHH-DPCs and mouse osteoblastic MC3T3-E1 cells. A mouse experimental 
      periodontitis model was used to evaluate the effect of exosomes in vivo. The 
      morphology of alveolar bone was assessed by micro-computed tomography (muCT) and 
      histological analysis. The effect of exosomes on osteoclastogenesis was evaluated 
      using a co-culture system. RESULTS: The exosomes purified from HHH-DPCs were 
      homogeneous and had a spherical membrane structure. HHH-DPC exosomes promoted the 
      migration of both human DPCs and mouse osteoblastic cells. The MTT assay showed a 
      positive effect on the proliferation of human DPCs, but not on mouse osteoblastic 
      cells. Treatment with HHH-DPC exosomes did not alter the differentiation of 
      osteoblastic cells. Imaging with microCT revealed that the exosomes suppressed 
      alveolar bone resorption in the mouse model of periodontitis. Although no change 
      was apparent in the dominance of TRAP-positive osteoclast-like cells in 
      decalcified tissue sections upon exosome treatment, HHH-DPC exosomes 
      significantly suppressed osteoclast formation in vitro. CONCLUSIONS: HHH-DPC 
      exosomes stimulated the migration of human DPCs and mouse osteoblastic cells and 
      effectively attenuated bone loss due to periodontitis.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Shimizu, Yuta
AU  - Shimizu Y
AUID- ORCID: 0000-0002-5341-9521
AD  - Division of Oral Infections and Health Sciences, Department of Periodontology, 
      Asahi University School of Dentistry, Gifu, Japan.
FAU - Takeda-Kawaguchi, Tomoko
AU  - Takeda-Kawaguchi T
AD  - Department of Oral Maxillofacial Surgery, Gifu University Graduate School of 
      Medicine, Gifu, Japan.
FAU - Kuroda, Izumi
AU  - Kuroda I
AD  - Department of Stem Cell and Regenerative Medicine, Gifu University Graduate 
      School of Medicine, Gifu, Japan.
FAU - Hotta, Yasuaki
AU  - Hotta Y
AD  - Central Research Institute of Oral Science, Asahi University School of Dentistry, 
      Gifu, Japan.
FAU - Kawasaki, Hideya
AU  - Kawasaki H
AD  - Institute for NanoSuit Research, Preeminent Medical Photonics Education & 
      Research Center, Hamamatsu University School of Medicine, Shizuoka, Japan.
FAU - Hariyama, Takahiko
AU  - Hariyama T
AD  - Institute for NanoSuit Research, Preeminent Medical Photonics Education & 
      Research Center, Hamamatsu University School of Medicine, Shizuoka, Japan.
FAU - Shibata, Toshiyuki
AU  - Shibata T
AD  - Department of Oral Maxillofacial Surgery, Gifu University Graduate School of 
      Medicine, Gifu, Japan.
FAU - Akao, Yukihiro
AU  - Akao Y
AD  - United Graduate School of Drug Discovery and Medical Information Sciences, Gifu 
      University, Gifu, Japan.
FAU - Kunisada, Takahiro
AU  - Kunisada T
AD  - Department of Stem Cell and Regenerative Medicine, Gifu University Graduate 
      School of Medicine, Gifu, Japan.
FAU - Tatsumi, Junichi
AU  - Tatsumi J
AD  - Division of Oral Infections and Health Sciences, Department of Periodontology, 
      Asahi University School of Dentistry, Gifu, Japan.
FAU - Tezuka, Ken-Ichi
AU  - Tezuka KI
AD  - Department of Stem Cell and Regenerative Medicine, Gifu University Graduate 
      School of Medicine, Gifu, Japan.
AD  - Center for Highly Advanced Integration of Nano and Life Sciences, Gifu University 
      (G-CHAIN), Gifu, Japan.
LA  - eng
GR  - JP17K11830/Japan Society for the Promotion of Science/
GR  - JP26293426/Japan Society for the Promotion of Science/
GR  - JP19K10263/Japan Society for the Promotion of Science/
GR  - JP20K10158/Japan Society for the Promotion of Science/
GR  - Ministry of Education, Culture, Sports, Science and Technology of Japan/
PT  - Journal Article
DEP - 20211126
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
SB  - IM
MH  - *Alveolar Bone Loss/diagnostic imaging/therapy
MH  - Animals
MH  - Cell Differentiation
MH  - Dental Pulp
MH  - *Exosomes
MH  - Mice
MH  - *Periodontitis/therapy
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - dental pulp cells
OT  - exosomes
OT  - ligature-induced mouse model
OT  - periodontal disease
EDAT- 2021/11/27 06:00
MHDA- 2022/01/19 06:00
CRDT- 2021/11/26 17:08
PHST- 2021/09/07 00:00 [received]
PHST- 2021/10/12 00:00 [accepted]
PHST- 2021/11/27 06:00 [pubmed]
PHST- 2022/01/19 06:00 [medline]
PHST- 2021/11/26 17:08 [entrez]
AID - 10.1111/jre.12949 [doi]
PST - ppublish
SO  - J Periodontal Res. 2022 Jan;57(1):162-172. doi: 10.1111/jre.12949. Epub 2021 Nov 
      26.