PMID- 34827254
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211129
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 10
IP  - 11
DP  - 2021 Oct 29
TI  - Antimicrobial Resistance and Molecular Epidemiological Characteristics of 
      Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of 
      Dental Patients and Staff in Northern Japan.
LID - 10.3390/antibiotics10111316 [doi]
LID - 1316
AB  - The acquisition of drug resistance and virulence by staphylococcal species 
      colonizing humans is a growing public health concern. The present study was 
      conducted to investigate the prevalence, antimicrobial resistance and genetic 
      characteristics of Staphylococcus isolates from the oral cavity and skin (hand) 
      of systemically healthy subjects with dental disease and dental staff in northern 
      Japan. Among a total of 133 subjects (91 patients and 42 staff), 87 
      coagulase-positive Staphylococcus (83 S. aureus/4 S. argenteus) and 162 
      coagulase-negative Staphylococcus (CoNS) isolates were recovered from 59 (44.4%) 
      and 95 (71.4%) subjects, respectively. Three oral isolates were 
      methicillin-resistant S. aureus (MRSA) (3.6%, 3/83) that were genotyped as 
      ST8-SCCmec-IVl, ST4775(CC1)-SCCmec-IVa and ST6562(CC8)-SCCmec-IVa. Remarkably, 
      the ST6562 isolate harbored PVL genes on PhiSa2usa and type I ACME (arginine 
      catabolic mobile element). Four methicillin-susceptible isolates were identified 
      as S. argenteus belonging to ST1223 and ST2250, which harbored enterotoxin genes 
      egc-2 and sey, respectively. Among the fourteen CoNS species identified, 
      methicillin-resistant (MR) isolates were detected in five species (11 isolates, 
      13.3% of CoNS), with S. saprophyticus and S. haemolyticus being the most common. 
      ACME was prevalent in only S. epidermidis and S. capitis. These findings 
      indicated the potential distribution of USA300 clone-like MRSA, toxigenic S. 
      argenteus and MR-CoNS in the oral cavity of dental patients.
FAU - Hirose, Mina
AU  - Hirose M
AD  - Division of Pediatric Dentistry, Department of Oral Growth and Development, 
      School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 
      061-0293, Japan.
FAU - Aung, Meiji Soe
AU  - Aung MS
AUID- ORCID: 0000-0002-8402-5349
AD  - Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 
      060-8556, Japan.
FAU - Fukuda, Atsushi
AU  - Fukuda A
AD  - Division of Pediatric Dentistry, Department of Oral Growth and Development, 
      School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 
      061-0293, Japan.
FAU - Yahata, Shoko
AU  - Yahata S
AD  - Division of Pediatric Dentistry, Department of Oral Growth and Development, 
      School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 
      061-0293, Japan.
FAU - Fujita, Yusuke
AU  - Fujita Y
AD  - Division of Pediatric Dentistry, Department of Oral Growth and Development, 
      School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 
      061-0293, Japan.
FAU - Saitoh, Masato
AU  - Saitoh M
AUID- ORCID: 0000-0002-9237-8363
AD  - Division of Pediatric Dentistry, Department of Oral Growth and Development, 
      School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 
      061-0293, Japan.
FAU - Hirose, Yukito
AU  - Hirose Y
AD  - Division of Fixed Prosthodontics and Oral Implantology, Department of Oral 
      Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 
      Ishikari-Tobetsu 061-0293, Japan.
FAU - Urushibara, Noriko
AU  - Urushibara N
AUID- ORCID: 0000-0003-0363-567X
AD  - Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 
      060-8556, Japan.
FAU - Kobayashi, Nobumichi
AU  - Kobayashi N
AUID- ORCID: 0000-0002-0146-6211
AD  - Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 
      060-8556, Japan.
LA  - eng
GR  - JP20H03933/Japan Society for the Promotion of Science/
GR  - JP21K10401/Japan Society for the Promotion of Science/
GR  - JP19K10450/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20211029
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC8615198
OTO - NOTNLM
OT  - ACME
OT  - MRSA
OT  - PVL
OT  - SCCmec
OT  - Staphylococcus argenteus
OT  - coagulase-negative Staphylococcus
OT  - enterotoxin
OT  - hand
OT  - oral cavity
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/28 06:00
MHDA- 2021/11/28 06:01
PMCR- 2021/10/29
CRDT- 2021/11/27 01:02
PHST- 2021/09/30 00:00 [received]
PHST- 2021/10/26 00:00 [revised]
PHST- 2021/10/26 00:00 [accepted]
PHST- 2021/11/27 01:02 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2021/11/28 06:01 [medline]
PHST- 2021/10/29 00:00 [pmc-release]
AID - antibiotics10111316 [pii]
AID - antibiotics-10-01316 [pii]
AID - 10.3390/antibiotics10111316 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2021 Oct 29;10(11):1316. doi: 10.3390/antibiotics10111316.