PMID- 34828284
OWN - NLM
STAT- MEDLINE
DCOM- 20220215
LR  - 20220215
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 12
IP  - 11
DP  - 2021 Oct 23
TI  - Associations of Polymorphisms Localized in the 3'UTR Regions of the KRAS, NRAS, 
      MAPK1 Genes with Laryngeal Squamous Cell Carcinoma.
LID - 10.3390/genes12111679 [doi]
LID - 1679
AB  - BACKGROUND: Genetic variations, localized in the 3' untranslated region (UTR) in 
      mitogen-activated protein kinase (MAPK) pathway-related genes, may alter the 
      transcription and impact the pathogenesis of laryngeal squamous cell carcinoma 
      (LSCC). The present study investigated the associations of single-nucleotide 
      polymorphisms (SNP), localized in the 3'UTR) of the KRAS, NRAS, and MAPK1 genes 
      with LSCC risk and clinicopathological features. METHODS: Genomic DNA and 
      clinical data were collected from 327 adult men with LSCC. The control group was 
      formed from 333 healthy men. Genotyping of the SNPs was performed using TaqMan 
      SNP genotyping assays. Five KRAS, NRAS, and MAPK1 polymorphisms were analyzed. 
      All studied genotypes were in Hardy-Weinberg equilibrium and had the same allele 
      distribution as the 1000 Genomes project Phase 3 dataset for the European 
      population. RESULTS: Significant associations of the studied SNPs with reduced 
      LSCC risk were observed between NRAS rs14804 major genotype CC. Significant 
      associations of the studied SNPs with clinicopathologic variables were also 
      observed between NRAS rs14804 minor T allele and advanced tumor stage and 
      positive lymph node status. SNP of MAPK1 rs9340 was associated with distant 
      metastasis. Moreover, haplotype analysis of two KRAS SNPs rs712 and rs7973450 
      revealed that TG haplotype was associated with positive lymph node status in LSCC 
      patients. CONCLUSIONS: According to the present study, 3'UTR SNP in the NRAS and 
      MAPK1 genes may contribute to the identifications of patients at higher risk of 
      LSCC lymph node and distant metastasis development.
FAU - Insodaite, Ruta
AU  - Insodaite R
AD  - Institute of Biology Systems and Genetic Research, Lithuanian University of 
      Health Sciences, 50103 Kaunas, Lithuania.
FAU - Smalinskiene, Alina
AU  - Smalinskiene A
AD  - Institute of Biology Systems and Genetic Research, Lithuanian University of 
      Health Sciences, 50103 Kaunas, Lithuania.
FAU - Liutkevicius, Vykintas
AU  - Liutkevicius V
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      50161 Kaunas, Lithuania.
FAU - Ulozas, Virgilijus
AU  - Ulozas V
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      50161 Kaunas, Lithuania.
FAU - Poceviciute, Roberta
AU  - Poceviciute R
AD  - Institute of Biology Systems and Genetic Research, Lithuanian University of 
      Health Sciences, 50103 Kaunas, Lithuania.
FAU - Bielevicius, Arunas
AU  - Bielevicius A
AD  - Institute of Biology Systems and Genetic Research, Lithuanian University of 
      Health Sciences, 50103 Kaunas, Lithuania.
FAU - Kucinskas, Laimutis
AU  - Kucinskas L
AD  - Institute of Biology Systems and Genetic Research, Lithuanian University of 
      Health Sciences, 50103 Kaunas, Lithuania.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211023
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (3' Untranslated Regions)
RN  - 0 (KRAS protein, human)
RN  - 0 (Membrane Proteins)
RN  - EC 2.7.11.24 (MAPK1 protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (NRAS protein, human)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
SB  - IM
MH  - 3' Untranslated Regions
MH  - Aged
MH  - Case-Control Studies
MH  - GTP Phosphohydrolases/*genetics
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Lithuania
MH  - Lymphatic Metastasis
MH  - Male
MH  - Membrane Proteins/*genetics
MH  - Middle Aged
MH  - Mitogen-Activated Protein Kinase 1/*genetics
MH  - Neoplasm Staging
MH  - *Polymorphism, Single Nucleotide
MH  - Proto-Oncogene Proteins p21(ras)/*genetics
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/*pathology
PMC - PMC8625477
OTO - NOTNLM
OT  - KRAS
OT  - MAPK1
OT  - NRAS
OT  - laryngeal squamous cell carcinoma
OT  - mitogen-activated protein kinases
OT  - single-nucleotide polymorphisms
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/28 06:00
MHDA- 2022/02/16 06:00
PMCR- 2021/10/23
CRDT- 2021/11/27 01:05
PHST- 2021/09/14 00:00 [received]
PHST- 2021/10/18 00:00 [revised]
PHST- 2021/10/21 00:00 [accepted]
PHST- 2021/11/27 01:05 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2022/02/16 06:00 [medline]
PHST- 2021/10/23 00:00 [pmc-release]
AID - genes12111679 [pii]
AID - genes-12-01679 [pii]
AID - 10.3390/genes12111679 [doi]
PST - epublish
SO  - Genes (Basel). 2021 Oct 23;12(11):1679. doi: 10.3390/genes12111679.