PMID- 34828325
OWN - NLM
STAT- MEDLINE
DCOM- 20220216
LR  - 20220216
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 12
IP  - 11
DP  - 2021 Oct 28
TI  - Analysis of SI-Related BoGAPDH Family Genes and Response of BoGAPC to SI Signal 
      in Brassica oleracea L.
LID - 10.3390/genes12111719 [doi]
LID - 1719
AB  - Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is not only involved in 
      carbohydrate metabolism, but also plays an important role in stress resistance. 
      However, it has not been reported in Brassica oleracea. In this study, we 
      performed a genome-wide identification of BoGAPDH in B. oleracea and performed 
      cloning and expression analysis of one of the differentially expressed genes, 
      BoGAPC. A total of 16 members of the BoGAPDH family were identified in B. 
      oleracea, which were conserved, distributed unevenly on chromosomes and had 
      tandem repeat genes. Most of the genes were down-regulated during 
      self-pollination, and the highest expression was found in stigmas and sepals. 
      Different transcriptome data showed that BoGAPDH genes were differentially 
      expressed under stress, which was consistent with the results of qRT-PCR. We 
      cloned and analyzed the differentially expressed gene BoGAPC and found that it 
      was in the down-regulated mode 1 h after self-pollination, and the expression was 
      the highest in the stigma, which was consistent with the result of GUS staining. 
      The promoter region of the gene not only has stress response elements and plant 
      hormone response elements, but also has a variety of specific elements for 
      regulating floral organ development. Subcellular localization indicates that the 
      BoGAPC protein is located in the cytoplasm and belongs to the active protein in 
      the cytoplasm. The results of prokaryotic expression showed that the size of the 
      BoGAPC protein was about 37 kDa, which was consistent with the expected results, 
      indicating that the protein was induced in prokaryotic cells. The results of 
      yeast two-hybrid and GST pull-down showed that the SRK kinase domain interacted 
      with the BoGAPC protein. The above results suggest that the BoGAPDH family of B. 
      oleracea plays an important role in the process of plant stress resistance, and 
      the BoGAPC gene may be involved in the process of self-incompatibility in B. 
      oleracea, which may respond to SI by encoding proteins directly interacting with 
      SRK.
FAU - Xie, Qinqin
AU  - Xie Q
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Zhang, Hecui
AU  - Zhang H
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Hu, Dengke
AU  - Hu D
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Liu, Qianying
AU  - Liu Q
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Zuo, Tonghong
AU  - Zuo T
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Zhang, Yizhong
AU  - Zhang Y
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Liu, Yimei
AU  - Liu Y
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Zhou, Siru
AU  - Zhou S
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
FAU - Zhu, Liquan
AU  - Zhu L
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211028
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (Plant Proteins)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Brassica/genetics/*growth & development/metabolism
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Plant/genetics
MH  - Cloning, Molecular/*methods
MH  - Conserved Sequence
MH  - Down-Regulation
MH  - Evolution, Molecular
MH  - Flowers/genetics/growth & development
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation, Plant
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*genetics/*metabolism
MH  - Molecular Weight
MH  - Multigene Family
MH  - Plant Proteins/genetics/metabolism
MH  - Pollination
MH  - Stress, Physiological
PMC - PMC8618600
OTO - NOTNLM
OT  - B. oleracea L.
OT  - GAPC
OT  - GAPDH
OT  - cloning and expression analysis
OT  - family expansion
OT  - self-incompatibility
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2021/11/28 06:00
MHDA- 2022/02/17 06:00
PMCR- 2021/10/28
CRDT- 2021/11/27 01:05
PHST- 2021/09/12 00:00 [received]
PHST- 2021/10/21 00:00 [revised]
PHST- 2021/10/27 00:00 [accepted]
PHST- 2021/11/27 01:05 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2022/02/17 06:00 [medline]
PHST- 2021/10/28 00:00 [pmc-release]
AID - genes12111719 [pii]
AID - genes-12-01719 [pii]
AID - 10.3390/genes12111719 [doi]
PST - epublish
SO  - Genes (Basel). 2021 Oct 28;12(11):1719. doi: 10.3390/genes12111719.