PMID- 34828805
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211130
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 10
IP  - 11
DP  - 2021 Oct 21
TI  - Customized Deep Eutectic Solvents as Green Extractants for Ultrasonic-Assisted 
      Enhanced Extraction of Phenolic Antioxidants from Dogbane Leaf-Tea.
LID - 10.3390/foods10112527 [doi]
LID - 2527
AB  - This study evaluates the application of eco-friendly deep eutectic solvents 
      (DESs) in the extraction of phenolic antioxidants from dogbane leaf-tea (DLT). 
      The results showed DESs with lower viscosity allowed an efficient extraction of 
      significantly higher contents of total phenolics or flavonoids. An innovative and 
      high-efficient solvent, choline chloride-levulinic acid (ChCl-LevA), was screened 
      and used in ultrasonic-assisted extraction (UAE) of phenolic compounds from DLT. 
      According to full factorial design experimental results, total phenolic content 
      (TPC), total flavonoid content (TFC), antioxidant activity, and 
      anti-alpha-glucosidase activity (alpha-GIA) of the DLT extracts were simultaneously 
      optimized by response surface methodology. Sonication temperature and water 
      content in ChCl-LevA were found to be the major factors affecting the TPC, TFC, 
      antioxidant activity, and alpha-GIA of DLT extracts. Under the optimum parameters 
      (water content in ChCl-LevA was 45%, sonication temperature was 50  degrees C, and 
      extraction time was 30 min), the measured results for all the responses were 
      obtained as follows: TPC-91.38 +/- 7.20 mg GAE/g DW, TFC-84.12 +/- 3.47 mg RE/g DW, 
      ABTS(+)-492 +/- 7.33 mmol TE/g DW, FRAP-6235 +/- 121 mumol Fe(II)/g DW and alpha-GIA-230 +/- 
      7.59 mmol AE/g DW, which were consistent with the predicted values. In addition, 
      strongly significant positive correlations were observed between TPC/TFC and 
      bio-activities of the DLT extracts. HPLC results indicated high contents of 
      (-)-epigallocatechin (4272 +/- 84.86 mug/g DW), catechin (5268 +/- 24.53 mug/g DW), 
      isoquercitrin (3500 +/- 86.07 mug/g DW), kaempferol 3-O-rutinoside (3717 +/- 97.71 
      mug/g DW), and protocatechuic acid (644 +/- 1.65 mug/g DW) were observed in the DLT 
      extracts. In contrast to other extraction methods, ChCl-LevA-based UAE yielded 
      higher TPC, TFC, individual phenolic contents, stronger antioxidant activity, and 
      alpha-GIA. Scanning electron microscope (SEM) analysis further confirmed that 
      ChCl-LevA-based UAE enhanced the disruption of cell wall structure, thereby 
      making more phenolic antioxidants released from DLT. In short, ChCl-LevA-based 
      UAE was confirmed to be an innovative and high-efficient method for extraction of 
      phenolic antioxidants from DLT. Dogbane leaves can be considered as a good tea 
      source rich in natural antioxidants.
FAU - Wang, Ruimin
AU  - Wang R
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
FAU - Zhang, Weimin
AU  - Zhang W
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
AD  - Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan 
      University, Haikou 570228, China.
AD  - Key Laboraory of Tropical Fruits and Vegetables Quality and Safety for State 
      Market Regulation, Hainan University, Haikou 570228, China.
FAU - He, Ruiping
AU  - He R
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
FAU - Li, Wu
AU  - Li W
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
AD  - Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan 
      University, Haikou 570228, China.
FAU - Wang, Lu
AU  - Wang L
AD  - School of Food Science and Engineering, Hainan University, Haikou 570228, China.
AD  - Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan 
      University, Haikou 570228, China.
LA  - eng
GR  - No. 2019RC009 and No. 320RC511/Natural Science Foundation of Hainan Province, 
      China/
GR  - No. KYQD1901/Scientific Research Foundation of Hainan University, China/
PT  - Journal Article
DEP - 20211021
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC8620010
OTO - NOTNLM
OT  - Dogbane leaf-tea
OT  - anti-glucosidase activity
OT  - antioxidant activity
OT  - deep eutectic solvents
OT  - phenolic compounds
OT  - response surface methodology
OT  - ultrasonic-assisted extraction
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/28 06:00
MHDA- 2021/11/28 06:01
PMCR- 2021/10/21
CRDT- 2021/11/27 01:07
PHST- 2021/09/15 00:00 [received]
PHST- 2021/10/11 00:00 [revised]
PHST- 2021/10/17 00:00 [accepted]
PHST- 2021/11/27 01:07 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2021/11/28 06:01 [medline]
PHST- 2021/10/21 00:00 [pmc-release]
AID - foods10112527 [pii]
AID - foods-10-02527 [pii]
AID - 10.3390/foods10112527 [doi]
PST - epublish
SO  - Foods. 2021 Oct 21;10(11):2527. doi: 10.3390/foods10112527.