PMID- 34829122
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211130
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 10
IP  - 11
DP  - 2021 Nov 17
TI  - Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in 
      Three Poultry Species by HPLC-MS/MS.
LID - 10.3390/foods10112841 [doi]
LID - 2841
AB  - A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) 
      method was developed to simultaneously analyze levamisole (LMS) and mebendazole 
      (MBZ) and its two metabolites, 5-hydroxymebendazole (HMBZ) and 
      2-amino-5-benzoylbenzimidazole (AMBZ), in poultry muscle (chicken, duck and 
      goose). In the sample preparation process, basic ethyl acetate was used as the 
      extraction agent, and the extracted samples were back-extracted with hydrochloric 
      acid, purified by Oasis MCX solid-phase extraction (SPE) cartridges, and 
      reconstituted in the initial mobile phase after being blown dry with nitrogen. 
      Chromatographic separation was performed on an Xbridge C(18) column (4.6 mm x 150 
      mm, 5 mum) with 0.1% formic acid in water and acetonitrile as the mobile phases, 
      and gradient elution was performed at a flow rate of 0.6 mL/min and a column 
      temperature of 35  degrees C. In blank poultry muscle samples, the spiked concentrations 
      of LMS, MBZ, HMBZ, and AMBZ were within the range of the limit of quantitation 
      (LOQ) to 25 mug/kg. The peak areas of the four target drugs had a good linear 
      relationship with the concentration, and the determination coefficient (R(2)) 
      values were higher than 0.9990. The average recoveries of LMS, MBZ, HMBZ, and 
      AMBZ were 86.77-96.94%; the intraday relative standard deviations (RSDs) were 
      1.75-4.99% at LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL, and 2.0 MRL; the 
      interday RSDs were 2.54-5.52%; and the LODs and LOQs were 0.04-0.30 mug/kg and 
      0.12-0.80 mug/kg, respectively.
FAU - Gao, Pengfei
AU  - Gao P
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - Zhang, Peiyang
AU  - Zhang P
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - Guo, Yawen
AU  - Guo Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - He, Zhaoyuan
AU  - He Z
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - Dong, Yuhao
AU  - Dong Y
AD  - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, 
      China.
FAU - Tang, Yayun
AU  - Tang Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - Guan, Fanxun
AU  - Guan F
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - Zhang, Tao
AU  - Zhang T
AUID- ORCID: 0000-0003-1170-1622
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - Xie, Kaizhou
AU  - Xie K
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
LA  - eng
PT  - Journal Article
DEP - 20211117
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC8624778
OTO - NOTNLM
OT  - chicken meat
OT  - duck meat
OT  - goose meat
OT  - validation method
OT  - veterinary drugs
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/28 06:00
MHDA- 2021/11/28 06:01
PMCR- 2021/11/17
CRDT- 2021/11/27 01:08
PHST- 2021/10/03 00:00 [received]
PHST- 2021/11/12 00:00 [revised]
PHST- 2021/11/14 00:00 [accepted]
PHST- 2021/11/27 01:08 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2021/11/28 06:01 [medline]
PHST- 2021/11/17 00:00 [pmc-release]
AID - foods10112841 [pii]
AID - foods-10-02841 [pii]
AID - 10.3390/foods10112841 [doi]
PST - epublish
SO  - Foods. 2021 Nov 17;10(11):2841. doi: 10.3390/foods10112841.