PMID- 34832765
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211130
IS  - 2072-666X (Print)
IS  - 2072-666X (Electronic)
IS  - 2072-666X (Linking)
VI  - 12
IP  - 11
DP  - 2021 Oct 31
TI  - Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly 
      Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy.
LID - 10.3390/mi12111353 [doi]
LID - 1353
AB  - This study developed low-cost and highly sensitive immunoassay devices possessing 
      the ability to rapidly analyze urine samples. Further, they can quantitatively 
      detect three biomarkers indicating renal injury: monocyte chemotactic protein 1 
      (MCP-1), angiotensinogen (AGT), and liver-type fatty acid binding protein 
      (L-FABP). The devices were used to successfully estimate the concentrations of 
      the three biomarkers in urine samples within 2 min; the results were consistent 
      with those obtained via conventional enzyme-linked immunosorbent assay (ELISA), 
      which requires several hours. In addition, the estimated detection limits for the 
      three biomarkers were comparable to those of commercially available ELISA kits. 
      Thus, the proposed and fabricated devices facilitate high-precision and frequent 
      monitoring of renal function.
FAU - Kasama, Toshihiro
AU  - Kasama T
AUID- ORCID: 0000-0001-6753-1509
AD  - Department of Bioengineering, Graduate School of Engineering, The University of 
      Tokyo, Tokyo 113-8654, Japan.
AD  - Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, 
      Nagoya University, Aichi 464-8603, Japan.
FAU - Sun, Miaomiao
AU  - Sun M
AD  - Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Aichi 464-8603, Japan.
FAU - Kaji, Noritada
AU  - Kaji N
AUID- ORCID: 0000-0002-9828-873X
AD  - Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, 
      Nagoya University, Aichi 464-8603, Japan.
AD  - Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 
      Fukuoka 819-0395, Japan.
FAU - Akiyama, Shin'ichi
AU  - Akiyama S
AD  - Division of Nephrology, Department of Internal Medicine, Nagoya University 
      Graduate School of Medicine, Aichi 466-8550, Japan.
FAU - Yuzawa, Yukio
AU  - Yuzawa Y
AD  - Department of Nephrology, Fujita Health University, Aichi 470-1192, Japan.
FAU - Tokeshi, Manabu
AU  - Tokeshi M
AUID- ORCID: 0000-0002-4412-2144
AD  - Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, 
      Nagoya University, Aichi 464-8603, Japan.
AD  - Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, 
      Hokkaido 060-0808, Japan.
FAU - Matsuo, Seiichi
AU  - Matsuo S
AD  - Division of Nephrology, Department of Internal Medicine, Nagoya University 
      Graduate School of Medicine, Aichi 466-8550, Japan.
FAU - Baba, Yoshinobu
AU  - Baba Y
AUID- ORCID: 0000-0002-3025-2255
AD  - Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, 
      Nagoya University, Aichi 464-8603, Japan.
AD  - Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Aichi 464-8603, Japan.
AD  - Health Research Institute, National Institute of Advanced Industrial Science and 
      Technology, Kagawa 761-0395, Japan.
LA  - eng
GR  - JP24245013/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20211031
PL  - Switzerland
TA  - Micromachines (Basel)
JT  - Micromachines
JID - 101640903
PMC - PMC8621389
OTO - NOTNLM
OT  - biomarker
OT  - diabetic nephropathy
OT  - immunoassay
OT  - microbead
OT  - microchannel device
OT  - urine
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/28 06:00
MHDA- 2021/11/28 06:01
PMCR- 2021/10/31
CRDT- 2021/11/27 01:19
PHST- 2021/09/28 00:00 [received]
PHST- 2021/10/30 00:00 [revised]
PHST- 2021/10/30 00:00 [accepted]
PHST- 2021/11/27 01:19 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2021/11/28 06:01 [medline]
PHST- 2021/10/31 00:00 [pmc-release]
AID - mi12111353 [pii]
AID - micromachines-12-01353 [pii]
AID - 10.3390/mi12111353 [doi]
PST - epublish
SO  - Micromachines (Basel). 2021 Oct 31;12(11):1353. doi: 10.3390/mi12111353.