PMID- 34834074
OWN - NLM
STAT- MEDLINE
DCOM- 20211210
LR  - 20220614
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 22
DP  - 2021 Nov 19
TI  - Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 
      (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC.
LID - 10.3390/molecules26226982 [doi]
LID - 6982
AB  - The content and composition of dietary supplements is of great interest due to 
      their increasing consumption and variety of available brand offered in the 
      market. Accurate determination of vitamins is important for the improvement of 
      dietary supplement quality and nutrition assessments. In this regard, the 
      simultaneous determination of vitamin D3 (calcitriol-CT and cholecalciferol-CHL) 
      and K2 (menaquinone-4-MK-4 and menaquinone-7-MK-7) in dietary supplements was 
      developed by using ultra-high-pressure liquid chromatography (UHPLC). The overall 
      runtime per sample was above 35 min, with the retention times of 2.40, 6.59, 
      7.06, and 32.6 min for vitamin D3 (CT and CHL) and vitamin K2 (MK-4 and MK-7), 
      respectively. The limits of detection and limits of quantification for the target 
      nutritional compounds ranged between 0.04-0.05 microg/mL, respectively. The 
      validation results indicated that the method had reasonable linearity (R(2) >/= 
      0.9990), good recovery (>82%), satisfactory intra-day precision (</=1.9%) and 
      inter-day precision (</=3.5%), and high selectivity and specificity. The validated 
      UHPLC method was demonstrated to be precise, accurate, and robust for the 
      simultaneous determination of vitamins D3 (CT and CHL) and K2 (MK-4 and MK-7) in 
      dietary supplements.
FAU - Becze, Anca
AU  - Becze A
AD  - Research Institute for Analytical Instrumentation, National Institute of Research 
      and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 
      Cluj-Napoca, Romania.
FAU - Babalau Fuss, Vanda Liliana
AU  - Babalau Fuss VL
AD  - Research Institute for Analytical Instrumentation, National Institute of Research 
      and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 
      Cluj-Napoca, Romania.
FAU - Scurtu, Daniela Alexandra
AU  - Scurtu DA
AUID- ORCID: 0000-0002-5941-1213
AD  - Research Institute for Analytical Instrumentation, National Institute of Research 
      and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 
      Cluj-Napoca, Romania.
FAU - Tomoaia-Cotisel, Maria
AU  - Tomoaia-Cotisel M
AD  - Faculty of Chemistry and Chemical Engineering, Research Center of Physical 
      Chemistry, Babes-Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca, 
      Romania.
FAU - Mocanu, Aurora
AU  - Mocanu A
AD  - Faculty of Chemistry and Chemical Engineering, Research Center of Physical 
      Chemistry, Babes-Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca, 
      Romania.
FAU - Cadar, Oana
AU  - Cadar O
AUID- ORCID: 0000-0002-0879-9211
AD  - Research Institute for Analytical Instrumentation, National Institute of Research 
      and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 
      Cluj-Napoca, Romania.
LA  - eng
GR  - PN-III-P2-2.1-PED-2019-3373/CCCDI-UEFISCDI, PNCDI III/
PT  - Journal Article
DEP - 20211119
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 11032-49-8 (Vitamin K 2)
RN  - 1C6V77QF41 (Cholecalciferol)
RN  - 27Y876D139 (menatetrenone)
RN  - 8427BML8NY (menaquinone 7)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Calcitriol/*analysis
MH  - Cholecalciferol/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Dietary Supplements/*analysis
MH  - Vitamin K 2/*analogs & derivatives/analysis
PMC - PMC8624398
OTO - NOTNLM
OT  - UHPLC
OT  - calcitriol
OT  - cholecalciferol
OT  - menaquinone-4
OT  - menaquinone-7
OT  - method validation
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/28 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/11/19
CRDT- 2021/11/27 01:23
PHST- 2021/10/29 00:00 [received]
PHST- 2021/11/13 00:00 [revised]
PHST- 2021/11/17 00:00 [accepted]
PHST- 2021/11/27 01:23 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/11/19 00:00 [pmc-release]
AID - molecules26226982 [pii]
AID - molecules-26-06982 [pii]
AID - 10.3390/molecules26226982 [doi]
PST - epublish
SO  - Molecules. 2021 Nov 19;26(22):6982. doi: 10.3390/molecules26226982.