PMID- 34834816 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211129 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 10 IP - 11 DP - 2021 Nov 13 TI - A Novel Distinct Genetic Variant of Tomato Torrado Virus with Substantially Shorter RNA1-Specific 3'Untranslated Region (3'UTR). LID - 10.3390/plants10112454 [doi] LID - 2454 AB - Tomato torrado virus (ToTV) induces severe systemic necrosis in Solanum lycopersicum. This work aimed at describing the genetic variability of necrosis-inducing ToTV-Wal'17 collected in 2017, derived from the ToTV-Wal'03 after long-term passages in plants. Sequence analyses of the ToTV-Wal'17 indicated twenty-eight single nucleotide substitutions in coding sequence of both RNAs, twelve of which resulted in amino acid changes in viral polyproteins. Moreover the sequencing data revealed that the 3'UTR of ToTV-Wal'17 RNA1 was 394 nts shorter in comparison to Wal'03. The performed sequence analyses revealed that 3'UTR of RNA1 of ToTV-Wal'17 is the most divergent across all previously described European isolates. FAU - Budziszewska, Marta AU - Budziszewska M AUID- ORCID: 0000-0002-7637-3191 AD - Department of Molecular Biology and Biotechnology, Institute of Plant Protection-National Research Institute, Wegorka 20 Street, 60-318 Poznan, Poland. FAU - Wieczorek, Przemyslaw AU - Wieczorek P AUID- ORCID: 0000-0002-3306-9763 AD - Department of Molecular Biology and Biotechnology, Institute of Plant Protection-National Research Institute, Wegorka 20 Street, 60-318 Poznan, Poland. LA - eng GR - 2016/21/D/NZ9/02468 and 2016/21/D/NZ9/02478/National Science Center/ PT - Journal Article DEP - 20211113 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC8621019 OTO - NOTNLM OT - 3'untranslated regions OT - genetic variability OT - tandem repeats OT - tomato torrado virus COIS- The authors declare no conflict of interest. EDAT- 2021/11/28 06:00 MHDA- 2021/11/28 06:01 PMCR- 2021/11/13 CRDT- 2021/11/27 01:25 PHST- 2021/10/04 00:00 [received] PHST- 2021/11/10 00:00 [revised] PHST- 2021/11/11 00:00 [accepted] PHST- 2021/11/27 01:25 [entrez] PHST- 2021/11/28 06:00 [pubmed] PHST- 2021/11/28 06:01 [medline] PHST- 2021/11/13 00:00 [pmc-release] AID - plants10112454 [pii] AID - plants-10-02454 [pii] AID - 10.3390/plants10112454 [doi] PST - epublish SO - Plants (Basel). 2021 Nov 13;10(11):2454. doi: 10.3390/plants10112454.