PMID- 34835356
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 9
IP  - 11
DP  - 2021 Oct 26
TI  - Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste 
      Stream Valorization for Bacterial Cellulose Production.
LID - 10.3390/microorganisms9112230 [doi]
LID - 2230
AB  - Komagataeibacter spp. has been used for the bioconversion of industrial wastes 
      and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies 
      have demonstrated the capacity of Komagataeibacter spp. in the biotransformation 
      of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived 
      monomers (LDMs) and acetate. In general, detoxification and BC synthesis from 
      lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards 
      tricarboxylic acid and gluconeogenesis, respectively. However, the related 
      molecular aspects have not yet been identified in Komagataeibacter spp. In this 
      study, we isolated a cellulose-producing bacterium capable of synthesizing BC in 
      a minimal medium containing crude glycerol, a by-product from the biodiesel 
      production process. The isolate, affiliated to Komagataeibacter genus, 
      synthesized cellulose in a minimal medium containing glucose (3.3 +/- 0.3 g/L), 
      pure glycerol (2.2 +/- 0.1 g/L) and crude glycerol (2.1 +/- 0.1 g/L). Genome assembly 
      and annotation identified four copies of bacterial cellulose synthase operon and 
      genes for redirecting the carbon from the central metabolic pathway to 
      gluconeogenesis. According to the genome annotations, a BC production route from 
      acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated 
      using acetate. We identified that when K. rhaeticus ENS9b was grown in a minimal 
      medium supplemented with acetate, BC production was not observed. However, in the 
      presence of readily utilizable substrates, such as spent yeast hydrolysate, 
      acetate supplementation improved BC synthesis.
FAU - Cannazza, Pietro
AU  - Cannazza P
AD  - Department of Food, Environmental and Nutritional Sciences (DeFENS), University 
      of Milan, Via Celoria 2, 20133 Milan, Italy.
AD  - Faculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, 
      Finland.
FAU - Rissanen, Antti J
AU  - Rissanen AJ
AD  - Faculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, 
      Finland.
FAU - Guizelini, Dieval
AU  - Guizelini D
AD  - Graduate Program in Bioinformatics, Sector of Professional and Technological 
      Education, Federal University of Parana (UFPR), Curitiba 81520-260, PR, Brazil.
FAU - Losoi, Pauli
AU  - Losoi P
AD  - Faculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, 
      Finland.
FAU - Sarlin, Essi
AU  - Sarlin E
AUID- ORCID: 0000-0002-2525-9639
AD  - Faculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, 
      Finland.
FAU - Romano, Diego
AU  - Romano D
AD  - Department of Food, Environmental and Nutritional Sciences (DeFENS), University 
      of Milan, Via Celoria 2, 20133 Milan, Italy.
FAU - Santala, Ville
AU  - Santala V
AD  - Faculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, 
      Finland.
FAU - Mangayil, Rahul
AU  - Mangayil R
AD  - Faculty of Engineering and Natural Sciences, Tampere University, 33720 Tampere, 
      Finland.
LA  - eng
GR  - 323214/Academy of Finland/
PT  - Journal Article
DEP - 20211026
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC8621423
OTO - NOTNLM
OT  - Komagataeibacter rhaeticus
OT  - acetate
OT  - bacterial cellulose
OT  - crude glycerol
OT  - minimal medium
OT  - whole-genome analysis
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/28 06:00
MHDA- 2021/11/28 06:01
PMCR- 2021/10/26
CRDT- 2021/11/27 01:27
PHST- 2021/08/13 00:00 [received]
PHST- 2021/10/21 00:00 [revised]
PHST- 2021/10/25 00:00 [accepted]
PHST- 2021/11/27 01:27 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2021/11/28 06:01 [medline]
PHST- 2021/10/26 00:00 [pmc-release]
AID - microorganisms9112230 [pii]
AID - microorganisms-09-02230 [pii]
AID - 10.3390/microorganisms9112230 [doi]
PST - epublish
SO  - Microorganisms. 2021 Oct 26;9(11):2230. doi: 10.3390/microorganisms9112230.