PMID- 34836105 OWN - NLM STAT- MEDLINE DCOM- 20211214 LR - 20211214 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 13 IP - 11 DP - 2021 Oct 28 TI - Lactic Acid Bacteria Fermented Cordyceps militaris (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model. LID - 10.3390/nu13113849 [doi] LID - 3849 AB - Cordyceps militaris (C. militaris) has various biomedical applications in traditional oriental medicine for different diseases including inflammatory and immune-dysregulated diseases. It is a reservoir of nutritional components such as cordycepin, polysaccharides, and antioxidants. To improve its bioactivity, we fermented C. militaris with a Pediococcus pentosaceus strain isolated from a salted small octopus (SC11). The current study aimed to evaluate whether P. pentosaceus (SC11) fermentation could enhance the anti-allergic potential of C. militaris cultured on germinated Rhynchosia nulubilis (GRC) against a type I hypersensitive reaction in in vitro and in vivo studies. Total antioxidant capacity and cordycepin content were significantly increased in GRC after SC11 fermentation. GRC-SC11 showed significantly enhanced anti-allergic responses by inhibiting immunoglobulin E (IgE)/antigen-induced degranulation in RBL-2H3 cells, compared to GRC. The results demonstrated the significant inhibition of phosphorylated spleen tyrosine kinase (Syk)/ p38/GRB2-associated binding protein 2 (Gab2)/c-jun in IgE/Ag-triggered RBL-2H3 cells. Furthermore, suppressed mRNA levels of interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-alpha) in IgE/Ag-activated RBL-2H3 cells were observed. GRC-SC11 significantly ameliorated IgE-induced allergic reactions by suppressing the ear swelling, vascular permeability, and inflammatory cell infiltration in passive cutaneous anaphylaxis (PCA) BALB/c mice. In conclusion, GRC fermented with P.pentosaceus exerted enhanced anti-allergic effects, and increased the cordycepin content and antioxidants potential compared to GRC. It can be used as bio-functional food in the prevention and management of type I allergic diseases. FAU - Phull, Abdul-Rehman AU - Phull AR AUID- ORCID: 0000-0002-4112-6588 AD - Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi-do, Korea. FAU - Dhong, Kyu-Ree AU - Dhong KR AD - Department of Life Science, College of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi-do, Korea. FAU - Park, Hye-Jin AU - Park HJ AD - Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi-do, Korea. LA - eng GR - 2021R1F1A1048156/National Research Foundation of Korea/ PT - Journal Article DEP - 20211028 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Anti-Allergic Agents) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Animals MH - Anti-Allergic Agents/*metabolism MH - Cell Culture Techniques MH - Cordyceps/*metabolism MH - Disease Models, Animal MH - Fermentation MH - Humans MH - Hypersensitivity/immunology/*microbiology/therapy MH - Immunoglobulin E/metabolism MH - Lactobacillales/*metabolism MH - Mast Cells/immunology/microbiology MH - Mice MH - Mice, Inbred BALB C MH - Pediococcus pentosaceus/*metabolism PMC - PMC8618942 OTO - NOTNLM OT - Cordyceps militaris OT - anti-allergy OT - fermentation COIS- There is no conflict of interest to declare. The funding providers had no role in the study design, execution, or publishing of the manuscript. EDAT- 2021/11/28 06:00 MHDA- 2021/12/15 06:00 PMCR- 2021/10/28 CRDT- 2021/11/27 01:29 PHST- 2021/09/23 00:00 [received] PHST- 2021/10/24 00:00 [revised] PHST- 2021/10/27 00:00 [accepted] PHST- 2021/11/27 01:29 [entrez] PHST- 2021/11/28 06:00 [pubmed] PHST- 2021/12/15 06:00 [medline] PHST- 2021/10/28 00:00 [pmc-release] AID - nu13113849 [pii] AID - nutrients-13-03849 [pii] AID - 10.3390/nu13113849 [doi] PST - epublish SO - Nutrients. 2021 Oct 28;13(11):3849. doi: 10.3390/nu13113849.