PMID- 34836987
OWN - NLM
STAT- MEDLINE
DCOM- 20220131
LR  - 20220131
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Nov 26
TI  - Subcellular localization of nucleocapsid protein of SFTSV and its assembly into 
      the ribonucleoprotein complex with L protein and viral RNA.
PG  - 22977
LID - 10.1038/s41598-021-01985-x [doi]
LID - 22977
AB  - Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging 
      bunyavirus that causes novel zoonotic diseases in Asian countries including 
      China, Japan, South Korea, and Vietnam. In phleboviruses, viral proteins play a 
      critical role in viral particle formation inside the host cells. Viral 
      glycoproteins (GPs) and RNA-dependent RNA polymerase (RdRp) are colocalized in 
      the Golgi apparatus and endoplasmic reticulum-Golgi intermediate compartment 
      (ERGIC). The nucleocapsid (N) protein was widely expressed in the cytoplasm, even 
      in cells coexpressing GP. However, the role of SFTSV N protein remains unclear. 
      The subcellular localization of SFTSV structural proteins was investigated using 
      a confocal microscope. Subsequently, minigenome and immunoprecipitation assays 
      were carried out. The N protein interacts with viral RNA (vRNA) and further shows 
      translational activity with RdRp which is L protein and localized in the ERGIC 
      and Golgi apparatus when co-expressed with GP. On the other hand, mutant N 
      protein did not interact with vRNA either localized in the ERGIC or Golgi 
      apparatus. The interaction between the N protein of SFTSV and vRNA is important 
      for the localization of viral proteins and viral assembly. This study provides 
      useful insights into the life cycle of SFTSV, which will lead to the detection of 
      antiviral targets.
CI  - (c) 2021. The Author(s).
FAU - Lokupathirage, Sithumini M W
AU  - Lokupathirage SMW
AD  - Graduate School of Infectious Diseases, Hokkaido University, Sapporo, 060-8638, 
      Japan.
FAU - Tsuda, Yoshimi
AU  - Tsuda Y
AD  - Department of Microbiology and Immunology, Faculty of Medicine, Hokkaido 
      University, Sapporo, 060-8638, Japan.
AD  - Graduate School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
FAU - Ikegame, Kodai
AU  - Ikegame K
AD  - Graduate School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
FAU - Noda, Kisho
AU  - Noda K
AD  - School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
FAU - Muthusinghe, Devinda S
AU  - Muthusinghe DS
AD  - Graduate School of Infectious Diseases, Hokkaido University, Sapporo, 060-8638, 
      Japan.
FAU - Kozawa, Fumiya
AU  - Kozawa F
AD  - School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
FAU - Manzoor, Rashid
AU  - Manzoor R
AD  - International Institute for Zoonosis Control, Hokkaido University, Sapporo, 
      001-0020, Japan.
FAU - Shimizu, Kenta
AU  - Shimizu K
AD  - Department of Microbiology and Immunology, Faculty of Medicine, Hokkaido 
      University, Sapporo, 060-8638, Japan.
AD  - Graduate School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
FAU - Yoshimatsu, Kumiko
AU  - Yoshimatsu K
AD  - Graduate School of Infectious Diseases, Hokkaido University, Sapporo, 060-8638, 
      Japan. yosimatu@igm.hokudai.ac.jp.
AD  - Institute for Genetic Medicine, Hokkaido University, Sapporo, 060-0815, Japan. 
      yosimatu@igm.hokudai.ac.jp.
LA  - eng
GR  - JP20jm0110019/Science and Technology Research Partnership for Sustainable 
      Development/
GR  - 21fk0108081h1303/Japan Agency for Medical Research and Development,/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211126
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (RNA, Viral)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Endoplasmic Reticulum/*metabolism
MH  - Golgi Apparatus/*metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Nucleocapsid Proteins/genetics/*metabolism
MH  - RNA, Viral/*metabolism
MH  - RNA-Dependent RNA Polymerase/genetics/*metabolism
MH  - Ribonucleoproteins/genetics/*metabolism
MH  - Severe Fever with Thrombocytopenia Syndrome/*metabolism
MH  - Vero Cells
MH  - Viral Envelope Proteins/genetics/*metabolism
PMC - PMC8626419
COIS- The authors declare no competing interests.
EDAT- 2021/11/28 06:00
MHDA- 2022/02/01 06:00
PMCR- 2021/11/26
CRDT- 2021/11/27 05:45
PHST- 2021/02/02 00:00 [received]
PHST- 2021/10/29 00:00 [accepted]
PHST- 2021/11/27 05:45 [entrez]
PHST- 2021/11/28 06:00 [pubmed]
PHST- 2022/02/01 06:00 [medline]
PHST- 2021/11/26 00:00 [pmc-release]
AID - 10.1038/s41598-021-01985-x [pii]
AID - 1985 [pii]
AID - 10.1038/s41598-021-01985-x [doi]
PST - epublish
SO  - Sci Rep. 2021 Nov 26;11(1):22977. doi: 10.1038/s41598-021-01985-x.