PMID- 34839833 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211203 IS - 1738-6055 (Print) IS - 2233-7660 (Electronic) IS - 1738-6055 (Linking) VI - 37 IP - 1 DP - 2021 Nov 29 TI - Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice. PG - 32 LID - 10.1186/s42826-021-00107-y [doi] LID - 32 AB - BACKGROUND: This study was undertaken to compare the sensitivities of mice strains during tumor induction by transcription activator-like effector nucleases (TALEN)-mediated Trp53 mutant gene. Alterations of their tumorigenic phenotypes including survival rate, tumor formation and tumor spectrum, were assessed in FVB/N-Trp53(em2Hwl)/Korl and C57BL/6-Trp53(em1Hwl)/Korl knockout (KO) mice over 16 weeks. RESULTS: Most of the physiological phenotypes factors were observed to be higher in FVB/N-Trp53(em2Hwl)/Korl KO mice than C57BL/6-Trp53(em1Hwl)/Korl KO mice, although there were significant differences in the body weight, immune organ weight, number of red blood cells, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), total bilirubin (Bil-T) and glucose (Glu) levels in the KO mice relative to the wild type (WT) mice. Furthermore, numerous solid tumors were also observed in various regions of the surface skin of FVB/N-Trp53(em2Hwl)/Korl KO mice, but were not detected in C57BL/6-Trp53(em1Hwl)/Korl KO mice. The most frequently observed tumor in both the Trp53 KO mice was malignant lymphoma, while soft tissue teratomas and hemangiosarcomas were only detected in the FVB/N-Trp53(em2Hwl)/Korl KO mice. CONCLUSIONS: Our results indicate that the spectrum and incidence of tumors induced by the TALEN-mediated Trp53 mutant gene is greater in FVB/N-Trp53(em2Hwl)/Korl KO mice than C57BL/6-Trp53(em1Hwl)/Korl KO mice over 16 weeks. CI - (c) 2021. The Author(s). FAU - Yun, Woo Bin AU - Yun WB AD - Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, Korea. AD - Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul, 03722, South Korea. FAU - Kim, Ji Eun AU - Kim JE AD - Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, Korea. FAU - Lee, Mi Lim AU - Lee ML AD - Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, Korea. FAU - Choi, Jun Young AU - Choi JY AD - Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, Korea. FAU - Park, Jin Ju AU - Park JJ AD - Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, Korea. FAU - Song, Bo Ram AU - Song BR AD - Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, Korea. FAU - Kang, Byeong Cheol AU - Kang BC AD - Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University College of Medicine, Seoul, 03080, Korea. FAU - Nam, Ki Taek AU - Nam KT AD - Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul, 03722, South Korea. FAU - Lee, Han-Woong AU - Lee HW AD - Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Korea. FAU - Hwang, Dae Youn AU - Hwang DY AUID- ORCID: 0000-0002-5144-1725 AD - Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, Korea. dyhwang@pusan.ac.kr. LA - eng PT - Journal Article DEP - 20211129 PL - England TA - Lab Anim Res JT - Laboratory animal research JID - 101560684 PMC - PMC8628475 OTO - NOTNLM OT - C57BL/6 OT - FVB/N OT - TALEN OT - Trp53 OT - Tumorigenesis COIS- The authors declare that they have no competing interests. EDAT- 2021/11/30 06:00 MHDA- 2021/11/30 06:01 PMCR- 2021/11/29 CRDT- 2021/11/29 05:35 PHST- 2021/07/27 00:00 [received] PHST- 2021/10/12 00:00 [accepted] PHST- 2021/11/29 05:35 [entrez] PHST- 2021/11/30 06:00 [pubmed] PHST- 2021/11/30 06:01 [medline] PHST- 2021/11/29 00:00 [pmc-release] AID - 10.1186/s42826-021-00107-y [pii] AID - 107 [pii] AID - 10.1186/s42826-021-00107-y [doi] PST - epublish SO - Lab Anim Res. 2021 Nov 29;37(1):32. doi: 10.1186/s42826-021-00107-y.