PMID- 34841314
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211130
IS  - 2666-5174 (Electronic)
IS  - 2666-5174 (Linking)
VI  - 2
DP  - 2021 Dec
TI  - Differential involvement of the canonical and noncanonical inflammasomes in the 
      immune response against infection by the periodontal bacteria Porphyromonas 
      gingivalis and Fusobacterium nucleatum.
PG  - 100023
LID - 10.1016/j.crmicr.2021.100023 [doi]
LID - 100023
AB  - We examined the involvement of the P2 x 7 receptor and the canonical and 
      noncanonical inflammasomes in the control of single-species or dual-species 
      infection by the periodontal bacteria Porphyromonas gingivalis and Fusobacterium 
      nucleatum in cells and mice. Stimulation of the P2 x 7 receptor leads to 
      activation of the canonical NLRP3 inflammasome and activation of caspase-1, which 
      leads to cleavage of pro-IL-1beta to IL-1beta, a key cytokine in the host inflammatory 
      response in periodontal disease. The non-canonical inflammasome pathway involves 
      caspase-11. Thus, wildtype (WT), P2 x 7(-/-), caspase-11(-/-) and 
      caspase-1/11(-/-) mice were co-infected with both bacterial species. In parallel, 
      bone marrow-derived macrophages (BMDMs) from WT mice and the different knockout 
      mice were infected with P. gingivalis and/or F. nucleatum, and treated or not 
      with extracellular ATP, which is recognized by P2 x 7. F. nucleatum infection 
      alone promoted secretion of IL-1beta in BMDMs. Conversely, the canonical pathway 
      involving P2 x 7 and caspase-1 was necessary for secretion of IL-1beta in BMDMs 
      infected with P. gingivalis and in the mandible of mice coinfected with P. 
      gingivalis and F. nucleatum. The P2 x 7 pathway can limit bacterial load in 
      single-species and dual-species infection with P. gingivalis and F. nucleatum in 
      BMDMs and in mice. The non-canonical pathway involving caspase-11 was required 
      for secretion of IL-1beta induced by F. nucleatum infection in BMDMs, without 
      treatment with ATP. Caspase-11 was also required for induction of cell death 
      during infection with F. nucleatum and contributed to limiting bacterial load 
      during F. nucleatum infection in BMDMs and in the gingival tissue of mice 
      coinfected with P. gingivalis and F. nucleatum. Together, these data suggest that 
      the P2 x 7-caspase-1 and caspase-11 pathways are involved in the immune response 
      against infection by P. gingivalis and F. nucleatum, respectively.
CI  - (c) 2021 The Authors.
FAU - De Andrade, Kivia Queiroz
AU  - De Andrade KQ
AD  - Immunobiology Program, Institute of Biophysics Carlos Chagas Filho, Federal 
      University of Rio de Janeiro, Edificio do Centro de Ciencias da Saude, Bloco G., 
      Av. Carlos Chagas Filho, 373, Cidade Universitaria, Ilha do Fundao, Rio de 
      Janeiro 21941-902, Brazil.
FAU - Almeida-da-Silva, Cassio Luiz Coutinho
AU  - Almeida-da-Silva CLC
AD  - Immunobiology Program, Institute of Biophysics Carlos Chagas Filho, Federal 
      University of Rio de Janeiro, Edificio do Centro de Ciencias da Saude, Bloco G., 
      Av. Carlos Chagas Filho, 373, Cidade Universitaria, Ilha do Fundao, Rio de 
      Janeiro 21941-902, Brazil.
AD  - Department of Biomedical Sciences, University of the Pacific, Arthur A. Dugoni 
      School of Dentistry, San Francisco, CA 94103, USA.
FAU - Ojcius, David M
AU  - Ojcius DM
AD  - Department of Biomedical Sciences, University of the Pacific, Arthur A. Dugoni 
      School of Dentistry, San Francisco, CA 94103, USA.
FAU - Coutinho-Silva, Robson
AU  - Coutinho-Silva R
AD  - Immunobiology Program, Institute of Biophysics Carlos Chagas Filho, Federal 
      University of Rio de Janeiro, Edificio do Centro de Ciencias da Saude, Bloco G., 
      Av. Carlos Chagas Filho, 373, Cidade Universitaria, Ilha do Fundao, Rio de 
      Janeiro 21941-902, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20210223
PL  - Netherlands
TA  - Curr Res Microb Sci
JT  - Current research in microbial sciences
JID - 101773003
PMC - PMC8610350
OTO - NOTNLM
OT  - Fusobacterium nucleatum
OT  - Inflammasome
OT  - Oral cavity
OT  - Porphyromonas gingivalis
OT  - Purinergic receptor
COIS- Robson Coutinho-Silva and David M. Ojcius serve on the editorial board of Current 
      Research in Microbial Sciences.
EDAT- 2021/11/30 06:00
MHDA- 2021/11/30 06:01
PMCR- 2021/02/23
CRDT- 2021/11/29 06:37
PHST- 2020/10/18 00:00 [received]
PHST- 2021/01/30 00:00 [revised]
PHST- 2021/02/16 00:00 [accepted]
PHST- 2021/11/29 06:37 [entrez]
PHST- 2021/11/30 06:00 [pubmed]
PHST- 2021/11/30 06:01 [medline]
PHST- 2021/02/23 00:00 [pmc-release]
AID - S2666-5174(21)00004-3 [pii]
AID - 100023 [pii]
AID - 10.1016/j.crmicr.2021.100023 [doi]
PST - epublish
SO  - Curr Res Microb Sci. 2021 Feb 23;2:100023. doi: 10.1016/j.crmicr.2021.100023. 
      eCollection 2021 Dec.